Abstract
Objective: To establish a dual real-time PCR method for the detection of orange and citrus components in orange juices. Methods: Through the design and screening of primers and probes for orange and citrus components. Through the detection of fruits, found the rule of fluorescence amplification of orange and citrus. Using the ratio difference of orange and citrus in FAM/VIC two fluorescence channels to determine the composition of orange and citrus. The lowest doping ratio detected by the method was verified by the experiment of orange juice adulteration, tested the commercial orange juice and verify the feasibility of the method. Results: There were fluorescence amplification curves in both FAM and Vic channels in pure orange fruits, and the fluorescence ratio of FAM/VIC channel was between 0.5±0.2, while only FAM channel had strong fluorescence amplification curve in pure citrus fruits. In the simulation experiment, it could be detected that there are more than 10% citrus juice in orange juice. Conclusion: The results showed that this method can be used to detect the orange and citrus components in commercial orange juices, and can be used to identify the fake orange juices.
Publication Date
8-28-2021
First Page
86
Last Page
93
DOI
10.13652/j.issn.1003-5788.2021.08.014
Recommended Citation
Yin-yin, CHEN; Juan, LI; Lu, ZHOU; Dan-xia, CHEN; Zhi-jie, HAN; and Qing-long, DING
(2021)
"Dual real-time fluorescence quantitative PCR method for the detection of orange and citrus components in orange juices,"
Food and Machinery: Vol. 37:
Iss.
8, Article 14.
DOI: 10.13652/j.issn.1003-5788.2021.08.014
Available at:
https://www.ifoodmm.cn/journal/vol37/iss8/14
References
[1] 牛灿杰, 张慧, 陈小珍. 果汁掺假鉴别检测技术研究进展[J]. 江苏农业科学, 2015, 43（6）: 292-294.
[2] 刘晗璐, 张九凯, 韩建勋, 等. 基于UPLC-QTOF-MS代谢组学技术的NFC和FC橙汁差异成分比较[J]. 食品科学, 2021, 42（6）: 229-237.
[3] 于丽薇, 蒋丽萍. 果汁饮料真假的鉴别方法[J]. 哈尔滨学院学报（教育）, 2002（8）: 66-67.
[4] 马耀宏, 史建国, 杨俊慧, 等. 果汁饮料中还原糖快速测定技术的研究[J]. 食品与发酵工业, 2006（6）: 104-106.
[5] 韩雅珊, 崔雨林. 柑桔类水果及其果汁中D-异柠檬酸的酶法测定[J]. 营养学报, 1996（1）: 120-125.
[6] D'ORAZIO Giovanni, CIFUENTES Alejandro, FANALI Salvatore. Chiral nano-liquid chromatography-mass spectrometry applied to amino acids analysis for orange juice profiling[J]. Food Chemistry, 2008, 108（3）: 1 114-1 121.
[7] PAN Geoffrey-g, KILMARTIN Paul-a, SMITH Bronwen-g, et al. Detection of orange juice adulteration by tangelo juice using multivariate analysis of polymethoxylated flavones and carotenoids[J]. Journal of the Science of Food and Agriculture, 2002, 82（4）: 421-427.
[8] ANTOLOVICH M, LI Xia, ROBARDS K. Detection of adulteration in australian orange juices by stable carbon isotope ratio analysis （SCIRA）[J]. Journal of Agricultural and Food Chemistry, 2001, 49（5）: 2 623-2 626.
[9] ERIC Jamin, REGIS Guérin, MELINDA Rétif, et al. Improved detection of added water in orange juice by simultaneous determination of the oxygen-18/oxygen-16 isotope ratios of water and ethanol derived from sugars[J]. Journal of Agricultural and Food Chemistry, 2003, 51（18）: 5 202-5 206.
[10] ZUO Rui-ting, ZHOU Si, ZUO Yue-gang, et al. Determination of creatinineuric and ascorbic acid in bovine milk and orange juice by hydrophilic interaction HPLC[J]. Food Chemistry, 2015, 182: 242-245.
[11] 李鑫, 刘柱, 蒋鑫, 等. 液相色谱/元素分析—同位素比率质谱联用法鉴定橙汁掺假[J]. 食品科技, 2013, 38（5）: 323-327.
[12] SIMPKINS Wayne-a, LOUIE Honway, WU Michael, et al. Trace elements in Australian orange juice and other products[J]. Food Chemistry, 2000, 71（4）: 423-433.
[13] 张淼, 贾洪锋, 李燮昕, 等. 电子舌在鲜榨橙汁掺假识别中的应用研究[J]. 食品与机械, 2015, 31（6）: 92-94, 207.
[14] 陈颖, 吴亚君. 话说我国果汁业中的果汁掺假问题[J]. 中国果业信息, 2013, 30（6）: 39-41.
[15] NG Chang-Chai, CHANG Chen-Chin, WU I-Chieh, et al. Rapid molecular identification of freshly squeezed and reconstituted orange juice[J]. International Journal of Food Science & Technology, 2006, 41（6）: 646-651.
[16] 梁宇斌, 牟靖芳, 李晓明, 等. 果汁中柑橘属成分实时荧光PCR检测方法[J]. 食品研究与开发, 2014, 35（16）: 84-90.
[17] 刘伟红, 许文涛, 商颖, 等. 果汁DNA提取方法比较及柑橘属植物分子生物学检测技术的研究[J]. 中国食品学报, 2012, 12（4）: 195-201.