Abstract
Objective: This study aimed to study the effect of Eurotium cristatum fermentation broth on improving the immunity of immunosuppressive mice. Methods: Immunosuppressive model was established by intraperitoneal injection of cyclophosphamide (80 mg/kg) in mice. Then the immunosuppressive mice were fed with low, middle and high dose fermentation broth for 15 days. The spleen index, carbon clearance test, half hemolysis value (HC50), delayed-type hypersensitivity test and the content of IL-2 in serum were detected to explore the changes of immunological indexes in immunosuppressive mice treated with fermentation broth. Results: Compared with the model group, the fermentation broth of the middle and high dose groups could significantly enhance the half hemolysis value (P＜0.05) and delayed hypersensitivity (P＜0.05) of immunosuppressive mice. Moreover, the fermentation broth of the low, middle and high dose groups could increase the content of IL-2 in serum, which was 3.07, 3.10, 2.87 times that of the regular group, respectively, but had no significant effect on the immune function of monocytes and macrophages in mice. Conclusion: E. cristatum fermentation broth could enhance the immunity of mice.
Publication Date
8-28-2021
First Page
169
Last Page
173
DOI
10.13652/j.issn.1003-5788.2021.08.029
Recommended Citation
Ying, PENG
(2021)
"The effects of Eurotium Cristatum fermentation broth on immunological indicators in immunosuppressive mice,"
Food and Machinery: Vol. 37:
Iss.
8, Article 29.
DOI: 10.13652/j.issn.1003-5788.2021.08.029
Available at:
https://www.ifoodmm.cn/journal/vol37/iss8/29
References
[1] XU Ai-qing, WANG Yuan-liang, WEN Jie-yu, et al. Fungal community associated with fermentation and storage of Fuzhuan brick-tea[J]. International Journal of Food Microbiology, 2011, 146（1）: 14-22.
[2] 吉杰丽, 杨勇, 袁丹丹, 等. 茯砖茶加工工艺及保健功效研究进展[J]. 食品与药品, 2016, 18（2）: 136-144.
[3] 曾超珍, 刘仲华. 茯砖茶抗氧化物质提取及其抗氧化活性研究[J]. 广东农业科学, 2015, 42（21）: 106-110.
[4] 曾桥, 张昊亮, 夏飞, 等. 茯砖茶提取物对秀丽隐杆线虫抗氧化及寿命的影响[J]. 陕西科技大学学报, 2019, 37（5）: 58-62.
[5] 黄颂, 刘仲华, 黄建安, 等. 茯茶水提物对Ⅱ型糖尿病小鼠糖代谢紊乱的干预作用[J]. 茶叶科学, 2016（3）: 250-260.
[6] 李玉婷, 吕嘉枥, 李良凤, 等. 冠突散囊菌固态发酵产消化酶活力的研究[J]. 中国酿造, 2012（10）: 93-95.
[7] 刘天囡, 徐梦佳, 胡冰, 等. 茯砖茶多酚类物质对胰脂肪酶活性的抑制作用[J]. 食品科学, 2015, 36（21）: 46-49.
[8] 李佳莲, 胡博涵, 赵勇彪, 等. 冠突散囊菌发酵液的抑菌作用[J]. 食品科学, 2011, 32（11）: 157-160.
[9] WANG Yuan-liang, XU Ai-qing, LIU Ping, et al. Effects of Fuzhuan brick-tea water extract on mice infected with E. coli O157: H7[J]. Nutrients, 2015, 7（7）: 5 309-5 326.
[10] 王许静. 二烯丙基三硫亚慢性毒性评价及其拮抗环磷酰胺毒性效果研究[D]. 济南: 山东大学, 2017: 1-2.
[11] 李晓冰, 展俊平, 张月腾, 等. 天麻多糖对环磷酰胺所致免疫功能低下小鼠体液免疫功能的影响[J]. 中国老年学杂志, 2016, 36（5）: 1 027-1 028.
[12] SHEEJA K, KUTTAN G. Protective effect of Andrographispaniculata and andrographolide on cyclophosphamide-induced urothelial toxicity[J]. Integrative Cancer Therapies, 2006, 5（3）: 244-251.
[13] 史晶晶, 时博, 苗明三. 黄芪多糖对环磷酰胺致免疫抑制小鼠免疫功能的影响[J]. 中医学报, 2016, 31（2）: 243-246.
[14] 李世杰, 李勇, 曾海英. 黑曲发酵茯苓多糖免疫调节作用研究[J]. 食品研究与开发, 2019, 40（1）: 37-41.
[15] 董升, 梁晗业, 王禹捷, 等. 车前草粗多糖对环磷酰胺所致免疫低下小鼠的免疫增强作用[J]. 食品工业科技, 2018, 39（18）: 289-293.
[16] 孟祥乐, 薛磊, 张振巍, 等. 马齿苋多糖对环磷酰胺模型小鼠免疫功能的影响[J]. 中国新药杂志, 2017, 26（11）: 1 296-1 300.
[17] 刘静, 李湘利, 许秀媛, 等. 茯砖茶中冠突散囊菌的分离及培养条件优化[J]. 食品科技, 2020, 45（10）: 41-45.
[18] 彭丽娜, 周旭雪, 王锴, 等. 玛咖对高脂饮食大鼠白细胞介素IL-1β, IL-2, IL-6, IL-8及免疫相关细胞的影响[J]. 免疫学杂志, 2018, 34（2）: 180-184.
[19] ZHANG Zhi-ying, PAN Ting, LIU Chun-rong, et al. Cyclophosphamide induced physiological and biochemical changes in mice with an emphasis on sensitivity analysis[J]. Ecotoxicology and Environmental Safety, 2021, 211: 111889.
[20] 颜爱, 李波, 李润成, 等. 香菇多糖和黄芪多糖对免疫抑制小鼠免疫功能调节的研究[J]. 中国免疫学杂, 2012, 28（11）: 999-1 001, 1 005.
[21] 刘龙龙, 陈致羽, 仇宏图, 等. 蓝莓提取物对小鼠免疫作用的研究[J]. 食品科技, 2020, 45（10）: 223-229.