ptimization of technological conditions for preparing corncob reducing sugar by two-enzyme method

Authors

WANG Jing-xuan, School of Food and Chemical Engineering, Shaoyang University, Shaoyang, Hunan 422000 , China
LU Bu-shi, School of Food and Chemical Engineering, Shaoyang University, Shaoyang, Hunan 422000 , China ；Hunan Key Laboratory of Ecological Brewing Technology and Application, Shaoyang, Hunan 422000 , China
LI Xin-she, School of Food and Chemical Engineering, Shaoyang University, Shaoyang, Hunan 422000 , China ；Hunan Key Laboratory of Ecological Brewing Technology and Application, Shaoyang, Hunan 422000 , China
WU Qiang, School of Food and Chemical Engineering, Shaoyang University, Shaoyang, Hunan 422000 , China ；Hunan Key Laboratory of Ecological Brewing Technology and Application, Shaoyang, Hunan 422000 , China

Abstract

Objective:This study focuses on realizing the reuse and resource optimization of corncobs.Methods:Using corncob as raw material, using cellulase and hemicellulase to synergistically degrade corncob to prepare reducing sugars. Based on single factor test, response surface methodology was used to determine the mass ratio of cellulase to hemicellulase and double-enzyme process conditions, including addition amount, enzymolysis time, enzymolysis temperature, were optimized.Results:The optimal process parameters for corncob degradation to produce reducing sugars were as follows. The mass ratio of cellulase to hemicellulase was 13∶2, and the amount of double enzyme added was3.25%, hydrolyzed at 50 ℃ for 5.0 h. The reducing sugar content in the corncob enzymatic hydrolysate prepared under the conditions could reach 12.45 mg/mL.Conclusion:The use of cellulase and hemicellulase to synergize the degradation of corncobs to efficiently and directionally prepare reducing sugars can achieve highvalue utilization of corncobs.

Publication Date

7-4-2022

First Page

203

Last Page

208

DOI

10.13652/j.issn.1003-5788.2022.01.034

Recommended Citation

Jing-xuan, WANG; Bu-shi, LU; Xin-she, LI; and Qiang, WU (2022) "ptimization of technological conditions for preparing corncob reducing sugar by two-enzyme method," Food and Machinery: Vol. 38: Iss. 1, Article 34.
DOI: 10.13652/j.issn.1003-5788.2022.01.034
Available at: https://www.ifoodmm.cn/journal/vol38/iss1/34

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