Abstract
Objective:This study aimed to elucidate the activities of endophytic bacteria and their metabolites of Gastrodia elata BI, and to provide theoretical reference for the development of new natural food antibacterial agents and antioxidants.Methods: The endophyte of fresh G. elata in Qinba area was isolated and purified by tissue homogenization and streaking method. The antibacterial, antioxidant and anti-tumor activities of the extract were determined. Furthermore, molecular biology identification was applied for the highly active strains.Results: In this study, four strains were isolated from the inside of fresh G. elata, among which, endophytic bacteria B1 and B2 have good antibacterial activity against Escherichia coli and Staphylococcus aureus . The inhibition zone diameter of endophytic bacteria B2 against S. aureus was 26.33 mm. The four strains all have good antioxidant activities, among which, the percentage of scavenging DPPH scavenges for endophytic bacteria B2 was as high as 69.13%. Endophytic bacteria strain B2, endophytic bacteria B3, and endophytic bacteria B4 have a potential inhibitory effect on HEK293T cells. Among them, endophytic bacteria B2 showed the highest inhibition rate which was 40.40%. The results of molecular biological identification showed that the endophytic bacteria strain B1 and B2 all belonged to Bacillus velezensis , while, endophytic bacteria B3 was Lelliottia sp., and endophytic bacteria B4 was Rahnella aquatilis .Conclusion: The endophyte of fresh G. elata in Qinba area was isolated and purified had antibacterial activity and antioxidant activity.
Publication Date
7-4-2022
First Page
36
Last Page
41
DOI
10.13652/j.issn.1003-5788.2022.01.005
Recommended Citation
Hai-yan, SUN; Xiao, LIU; Jin-jin, PEI; Dan-qing, HAO; and Er-nan, WANG
(2022)
"Isolation and identification of endophytic bacteria from fresh Gastrodia elata and preliminary study on its functional activity,"
Food and Machinery: Vol. 38:
Iss.
1, Article 5.
DOI: 10.13652/j.issn.1003-5788.2022.01.005
Available at:
https://www.ifoodmm.cn/journal/vol38/iss1/5
References
[1] 许廷生,陆龙存,黄子冬.天麻有效成分的药理作用分析与临床应用研究进展[J].中医临床研究,2020,12(21):133-135.XU Ting-sheng,LU Long-cun,HUANG Zi-dong.Progress in pharmacological action analysis and clinical application of active components of Gastrodia elata[J].Clinical Journal of Chinese Medicine,2020,12(21):33-135.
[2] 莫莉,康冀川,何劲,等.天麻内生真菌菌群组成的初步研究[J].菌物研究,2008,6(4):211-215.MO Li,KANG Ji-chuan,HE Jin,et al.A preliminary study on composition of endophytic fungi from Gastrodia elata[J].Journal of Fungal Research,2008,6(4):211-215.
[3] 熊城.荥经天麻内生真菌的分离与鉴定[D].雅安:四川农业大学,2017:16.XIONG Cheng.Separation and identification of endophytic fungi from Yingjing Gastrodia elata[D].Ya'an:Sichuan Agricultural University,2017:16.
[4] 余丽,李龙杰.昭通小草坝天麻内生真菌的分离初探[J].保山学院学报,2019,38(2):23-26.YU Li,LI Long-jie.Isolation of endophytic fungi from Gastrodia elata in Zhaotong Xiaocaoba[J].Journal of Baoshan University,2019,38(2):23-26.
[5] 郑旭.马铃薯内生菌的分离鉴定及抑龙葵碱功能研究[D].北京:中国农业科学院,2019:11.XU Zheng.Isolation and identification of endophytes from potato tubers and inhibition on solanine functions[D].Beijing:Chinese Academy of Agricultural Sciences,2019:11.
[6] 田双娥.三种不同抑菌圈试验法在牛至油抑菌评价中的应用研究[J].香料香精化妆品,2019(1):37-41.TIAN Shuang-e.Study on three kinds of inhibition zone tests applied in evaluation of antifungal activity of oregano oil[J].Flavour Fragrance Cosmetics,2019(1):37-41.
[7] 卢占慧,周如军,袁月,等.人参内生拮抗细菌分离,鉴定及其对人参菌核病抑菌作用研究[J].中国植保导刊,2016(3):6-9.LU Zhan-hui,ZHOU Ru-jun,YUAN Yue,et al.Isolation and identification of endophytic bacteria from Ginseng and its inhibition activity against Sclerotinia ginseng[J].China Plant Protection,2016(3):6-9.
[8] SHARMA O P,BHAT T K.DPPH antioxidant assay revisited[J].Food Chemistry,2009,113(4):1 202-1 205.
[9] 杜晓宁,代金霞.宁夏枸杞内生菌抗氧化活性菌株的筛选与鉴定[J].中国中药杂志,2015,40(20):3 941-3 944.DU Xiao-ning,DAI Jin-xia.Screening and identification of antioxidant endophytes from Lycium barbarum of Ningxia[J].China Journal of Chinese Materia Medica,2015,40(20):3 941-3 944.
[10] 陈子涵,刘金娟.六种食用菌体外抗氧化及抗细胞增殖活性研究[J].生物技术通报,2019,35(11):104-108.CHEN Zi-han,LIU Jin-juan.Invitro antioxidative and anti-proliferative activities of extractions from six common edible mushrooms[J].Biotechnology Bulletin,2019,35(11):104-108.
[11] 赵满仓,魏文青,刘晶,等.MTT法抗肿瘤药敏试验影响因素的探讨[J].临床肿瘤学杂志,2009,14(4):306-308.ZHAO Man,WEI Wen-qing,LIU Jing,et al.Study on the influencing facmrs of the MTT based tumor chemosensitivity assay in vitro[J].Chinese Clinical Oncology Apr,2009,14(4):306-308.
[12] 肖妍,刘芸宏,高贵田,等.PMA-qPCR方法检测陕西猕猴桃溃疡菌优势病原菌活菌的研究[J].食品与机械,2019,35(4):48-53,59.XIAO Yan,LIU Yun-hong,GAO Gui-tian,et al.PMA-qPCR for detecting live bacteria of Shaanxi kiwifruit cancer dominant pathogen[J].Food & Machinery,2019,35(4):48-53,59.
[13] SHAH S,SHRESTHA R,MAHARJAN S,et al.Isolation and characterization of plant growth-promoting endophytic fungi from the roots of dendrobium moniliforme[J].Plants,2018,8(1):5.
[14] 安传伟,石广娅,安传相,等.贵州野生天麻抗菌提取物外抑菌动力学研究[J].中医学报,2013,28(11):1 695-1 697.AN Chuan-wei,SHI Guang-ya,AN Chuan-xiang,et al.Antibacterialkinetic study on antibacterial composition of Guizhou wild Gastrodia elata blume in vitro[J].Journal of Chinese Medicine,2013,28(11):1 695-1 697.
[15] 陈伊铃.黄芩及天麻有效成分提取工艺优化和抗菌活性研究[D].长春:吉林大学,2017:15.CHEN Yi-ling.Study on extraction process optimization 〖JP2〗and antibacterial activity of scuttellaria baicalensis Georgi and Gastrodia[D].〖JP〗Changchun:Jilin University,2017:15.
[16] 王雪,逯家辉,王雪,等.天麻蛋白提取工艺优化及抑菌活性研究[J].中国食品学报,2018,18(4):176-182.WANG Xue,LU Jia-hui,WANG Xue,et al.Optimization of the extraction of Gastrodia elata protein and its antibacterial[J].Journal of Chinese Institute of Food Science and Technology,2018,18(4):176-182.
[17] 张梦娟.天麻多糖的提取、纯化及活性研究[D].咸阳:西北农林科技大学,2007:23.ZHANG Meng-juan.Study on extraction,purification and activity of polysaccharide in Gastrodia elata blume[D].Xianyang Northwest A & F University,2007:23.
[18] 陈琛,胡红忠,李鑫鑫,等.天麻多糖的分离纯化与抗氧化活性研究[J].中国临床药理学杂志,2018,34(18):2 203-2 206.CHEN Chen,HU Hong-zhong,LI Xin-xin,et al.Extraction,purification and antioxidant activity of polysaccharides from Gastrodia elata blume[J].The Chinese Journal of Clinical Pharmacology,2018,34(18):2 203-2 206.
[19] 张格超.天麻多糖分离纯化及其抗氧化活性研究[D].咸阳:西北农林科技大学,2015:31.ZHANG Ge-chao.Study on separation and purification and antioxidant activity of polysaccharides of Gastrodia elata[D].Xianyang:Northwest A & F University,2015:31.