Identification of psoraleae fructus illegally added in mixed wine and mixed tea based on high resolution mass spectrometry library and diagnostic ratio of characteristic

Authors

GAO Fang, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430075 , China
WU Wan-qin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430075 , China
WANG Bin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430075 , China
LIU Guo-jiao, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430075 , China
JIANG Feng, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test Office, Wuhan, Hubei 430075 , China

Abstract

Objective:A method based on the high resolution mass spectrometry library and diagnostic ratio of characteristic components was established to identify the illegally added psoraleae fructus in wine and tea.Methods:High resolution mass spectrometry library of psoraleae fructus was constructed by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-TOF-MS) for preliminary screening. Combined with the diagnostic ratio method of the characteristic components by, high performance liquid chromatography, psoraleae fructus in different 20 samples of mixed wine and mixed tea from different picking time and origin was tested and analyzed The diagnostic ratio of characteristic component peak area, which was less affected by the origin, picking time and processing technology, has no significant difference between different matrix groups, and was selected as the identification index.Results:High resolution mass spectrometry library of psoraleae fructus was established by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-TOF-MS) for preliminary screening. Corylia/psoralen and psoralen/isopsoralen were selected as the two characteristic groups. The box diagram was drawn to determine that the non-abnormal value range of Corylia/Psoralen was 0.061~0.115, and the non-abnormal value range of Psoralen/Isopsoralen was 1.14~1.68. The established screening and confirmatory methods were validated by commercial samples.Conclusion:The method is simple and efficient, which provides technical support for cracking down on illegal addition of psoraleae fructus in wine and tea.

Publication Date

7-4-2022

First Page

59

Last Page

65

DOI

10.13652/j.issn.1003-5788.2022.01.009

Recommended Citation

Fang, GAO; Wan-qin, WU; Bin, WANG; Guo-jiao, LIU; and Feng, JIANG (2022) "Identification of psoraleae fructus illegally added in mixed wine and mixed tea based on high resolution mass spectrometry library and diagnostic ratio of characteristic," Food and Machinery: Vol. 38: Iss. 1, Article 9.
DOI: 10.13652/j.issn.1003-5788.2022.01.009
Available at: https://www.ifoodmm.cn/journal/vol38/iss1/9

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