•  
  •  
 

Abstract

Objective: To obtain a promising production technique of conjugated linoleic acid (CLA). Methods: The alkali isomerization was used to prepare CLA from peanut oil. The relative content of linoleic acid in peanut oil was determined, and the effects of reaction time, reaction temperature, ratio of alcohol to oil and KOH dosage on the conversion of CLA were investigated. The preparation conditions were optimized by Box-Behnken test. Results: The peanut oil was rich in linoleic acid with a relative content of 27.85%. Therefore, peanut oil was a good raw material for the preparation of CLA. For the conversion of CLA, reaction temperature, reaction time and KOH dosage were the most significant factors (P<0.01), while ratio of propylene glycol to oil was the significant factor (P<0.05). There existed interaction between reaction time and ratio of propylene glycol to oil. The best conditions for preparation of CLA by alkali isomerization of peanut oil were as followed: the reaction temperature of 200 ℃, the reaction time of 2.6 h, the ratio of propylene glycol to oil of 22 to 1, the KOH dosage of 7% (mass fraction). Under determined conditions, the conversion of CLA was 89.81%. Conclusion: It is workable to prepare CLA by alkali isomerization of peanut oil, and the conversion of CLA is high under the optimal conditions.

Publication Date

12-28-2022

First Page

139

Last Page

144

DOI

10.13652/j.spjx.1003.5788.2022.80614

References

[1] 杨媚,马杰,杨泰,等.共轭亚油酸的生物学功能及其在动物生产中的应用[J].中国畜牧兽医,2019,46(11):3 216-3 224.YANG M,MA J,YANG T,et al.Biological function of conjugated linoleic acid and its application in animal production[J].China Animal Husbandry and Veterinary Medicine,2019,46(11):3 216-3 224.
[2] SALSINHA A S,PIMENTEL L L,FONTES A L,et al.Microbial production of conjugated linoleic acid and conjugated linolenic acid relies on a multi-enzymatic system[J].Microbiology and Molecular Biology Reviews,2018,82(4):e00019-18.
[3] 刘华鼐,张萌,唐小月,等.茶籽油制备共轭亚油酸的工艺优化及分离纯化研究[J].粮食与油脂,2020,33(11):51-55.LIU H N,ZHANG M,TANG X Y,et al.Study on process optimization and separation and purification of conjugated linoleic acid from camellia seed oil[J].Cereals & Oils,2020,33(11):51-55.
[4] 赵微,张峰,张和平,等.植物乳杆菌P-8转化亚油酸为共轭亚油酸的分析[J].食品科学,2021,42(10):94-103.ZHAO W,ZHANG F,ZHANG H P,et al.Conversion of linoleic acid to conjugated linoleic acid by Lactobacillus plantarump-8[J].Food Science,2021,42(10):94-103.
[5] LIAN W,LI D,ZHANG L,et al.Synthesis of conjugated linoleic acid-rich triacylglycerols by immobilized mutant lipase with excellent capability and recycl ability[J].Enzyme and Microbial Technology,2018,117:56-63.
[6] 刘书来,张生,王勇,等.Ru/Al2O3催化植物油异构化制备共轭亚油酸的研究[J].中国粮油学报,2017,32(11):70-75.LIU S L,ZHANG S,WANG Y,et al.Conjugated linoleic acid formation by isomerization of cottonseed oil over Ru/Al2O3 catalyst[J].Journal of the Chinese Cereals and Oils Association,2017,32(11):70-75.
[7] 马琳,樊晔,吴业帆,等.天然不饱和脂肪酸改性制备共轭亚油酸[J].大豆科学,2012,31(5):846-851.MA L,FAN Y,WU Y F,et al.Preparation of conjugated linoleic acids from natural unsaturated fatty acids[J].Soybean Science,2012,31(5):846-851.
[8] SAN K N E,FAN Y,FANG Y,et al.A soap-free strategy for one-pot synthesizing conjugated linoleic acid:Alkali isomerization of linoleic acid through preferential esterification pathway[J].Journal of the American Oil Chemists Society,2020,97(9):1 021-1 028.
[9] ALAALVAR H,ERINC H,SALUR F,et al.Production of conjugated linoleic acid by microwave-assisted and ultrasound-assisted alkali isomerization:Effects of microwave power and ultrasound amplitude[J].Journal of the American Oil Chemists Society,2019,96(7):839-846.
[10] SILVA-RAMIREZ A S,ROCHA-URIBE A,GONZALEZ-CHAVEZ M M,et al.Synthesis of conjugated linoleic acid by microwave-assisted alkali isomerization using propylene glycol as solvent[J].European Journal of Lipid Science and Technology,2017,119(4):1600079.
[11] 李静,王永,杨耀东,等.棕榈油与常见食用油脂肪酸组分的比较分析[J].南方农业学报,2016,47(12):2 124-2 128.LI J,WANG Y,YANG Y D,et al.Comparison of fatty acid component between palm oil and common edible oils[J].Journal of Southern Agriculture,2016,47(12):2 124-2 128.
[12] NIEZGODA N,GLISZCZYNSKA A,GLADKOWSKI W,et al.Production of concentrates of CLA obtained from sunflower and safflower and their application to the lipase-catalyzed acidolysis of egg yolk phosphatidylcholine[J].European Journal of Lipid Science and Technology,2016,118(10):1 566-1 578.
[13] 马燕,许铭强,李喜弟,等.响应面法优化辣椒籽油碱异构化制备共轭亚油酸工艺及其氧化稳定性[J].新疆农业科学,2022,59(4):908-915.MA Y,XU M Q,LI X D,et al.Optimization of preparation of conjugated linoleic acid by alkali isomerization of pepper seed oil by response surface methodology and its oxidation stability[J].Xinjiang Agricultural Sciences,2022,59(4):908-915.
[14] 张鹤.花生脂肪酸近红外模型的建立及高油酸种质的筛选[D].长春:吉林农业大学,2017:22-23.ZHANG H.The establishment of near-infrared model of peanut fatty acids and the screening of high oleic acid germplasm[J].Changchun:Jilin Agricultural University,2017:22-23.
[15] 郑云武,杨晓琴,黄元波,等.橡胶籽油碱催化异构化合成共轭亚油酸的制备工艺研究[J].食品工业科技,2016,37(16):253-258.ZHENG Y W,YANG X Q,HUANG Y B,et al.Study on preparation technology of conjugated linolenic acid of rubber seed oil by alkali catalytic isomerization[J].Science and Technology of Food Industry,2016,37(16):253-258.
[16] 彭永健,许新德,张莉华,等.红花籽油制备共轭亚油酸工艺优化及其组成分析[J].中国油脂,2015,40(6):72-76.PENG Y J,XU X D,ZHANG L H,et al.Optimization of preparation of conjugated linoleic acid from safflower seed oil and its composition analysis[J].China Oils and Fats,2015,40(6):72-76.
[17] 刘芸,苏平,宋思圆.响应面法优化黄秋葵籽油中亚油酸的共轭转化率[J].食品工业科技,2015,36(24):273-277.LIU Y,SU P,SONG S Y.Optimization of conjugate conversion rate of linoleic acid in okra seed oil by response surface method[J].Science and Technology of Food Industry,2015,36(24):273-277.
[18] 张绍绍,张慧,李昊昊,等.葵花油碱异构化法制备共轭亚油酸研究[J].中国食品添加剂,2019,30(9):100-105.ZHANG S S,ZHANG H,LI H H,et al.Study on preparation of conjugated linoleic acid by alkali isomerization of sunflower oil[J].China Food Additives,2019,30(9):100-105.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.