Refining isoflavones from Puerariae flos and its in vitro lipid-lowering activity

Authors

WU Wei, Food and Biological Technology College, Changchun Vocational Institute of Technology, Changchun, Jilin 130033 , China
LI Qun, Food and Biological Technology College, Changchun Vocational Institute of Technology, Changchun, Jilin 130033 , China
SONG Di, Food and Biological Technology College, Changchun Vocational Institute of Technology, Changchun, Jilin 130033 , China
SUN Ming-zhe, Food and Biological Technology College, Changchun Vocational Institute of Technology, Changchun, Jilin 130033 , China

Abstract

Objective:The refining parameters of isoflavones from Puerariae flos were optimized by using HPD-100 macroporous adsorption resin, and its in vitro lipid-lowering effects were evaluated. Methods:The optimized refine parameters of isoflavones from Puerariae flos were studied by macroporous adsorption resin, and the lipid-lowering effects of isoflavones from Puerariae flos were evaluated by pancreatic lipase enzyme activity, pancreatic cholesterol esterase enzyme activity and micellar cholesterol solubility. Results:Adsorption parameters: Puerariae flos isoflavones were adsorbed with concentration of 100 mg/mL, pH 6.0 and upper column volume of 20 mL. Desorption parameters: the adsorption resins were washed by 70% ethanol with pH 5.0 at flow rate of 2.0 mL/min. The purity of refined Puerariae flos isoflavones were 81.47%. The inhibition ratio on pancreatic lipase enzyme, pancreatic cholesterol esterase enzyme and micellar cholesterol solubility were 40.73%, 52.32% and 46.17%, respectively, and these were increased by 26.65%, 47.46% and 37.53% respectively, compared with not refining. Conclusion:Puerariae flos isoflavones showed lipid-lowering effects by inhibiting pancreatic lipase enzyme activity, pancreatic cholesterol esterase enzyme activity and micellar cholesterol solubility.

Publication Date

7-7-2022

First Page

180

Last Page

185

DOI

10.13652/j.issn.1003-5788.2022.02.030

Recommended Citation

Wei, WU; Qun, LI; Di, SONG; and Ming-zhe, SUN (2022) "Refining isoflavones from Puerariae flos and its in vitro lipid-lowering activity," Food and Machinery: Vol. 38: Iss. 2, Article 30.
DOI: 10.13652/j.issn.1003-5788.2022.02.030
Available at: https://www.ifoodmm.cn/journal/vol38/iss2/30

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