Abstract
Objective:This study aimed to develop new rice bran products.Methods:The ACE inhibition rate was used as an indicator, and the rice bran protein was optimized by single factor and response surface experiments. The optimal enzyme hydrolysate peptides were separated by ultrafiltration, activity evaluation and amino acid composition analysis were performed.Results:The optimal process conditions were as follows: pH 7.2, mass substrate concentration 8.2 g/100 mL, enzymolysis at 46 ℃ for 3 h, and enzyme addition 0.3 g/100 g rice bran protein. Under the control of these conditions, the ACE inhibition rate of the enzymatic hydrolysate was (73.15±0.64)%, and enzymatic hydrolysate was rich in hydrophobic amino acids (23.09 g/100 g). Activity analysis showed that the ACE inhibitory activity (81.68±1.08)% of ＜3 kDa active peptide component at the same concentration (1.0 mg/mL) was better than those of ＞3 kDa active peptide component (58.65±2.21)% and (72.64± 1.61)%.Conclusion:The rice bran proteolysate obtained under the optimal process conditions has significant ACE inhibitory activity, and the molecular weight of the active peptide component significantly affects its ACE inhibitory activity.
Publication Date
7-7-2022
First Page
160
Last Page
166
DOI
10.13652/j.spjx.1003.5788.2022.90047
Recommended Citation
Ling-yu, ZHANG; Jian-yin, MIAO; Yu, CAO; Wei-jie, DAI; Guo, LIU; Zhen-wei, LI; and Yong, CAO
(2022)
"Enzymatic preparation and activity study of rice bran protein-derived ACE inhibitory peptides,"
Food and Machinery: Vol. 38:
Iss.
3, Article 26.
DOI: 10.13652/j.spjx.1003.5788.2022.90047
Available at:
https://www.ifoodmm.cn/journal/vol38/iss3/26
References
[1] 唐金平,徐强.中医治疗高血压病的研究进展[J].光明中医,2021,36(6):1 007-1 009.TANG Jin-ping,XU Qiang.Research progress on traditional Chinese medicine in treating hypertension[J].Guangming Journal of Chinese Medicine,2021,36(6):1 007-1 009.
[2] 中国心血管健康与疾病报告2019概要[J].中国循环杂志,2020,35(9):833-854.Report on cardiovascular health and diseases in China 2019:An updated summary[J].Chinese Circulation Journal,2020,35(9):833-854.
[3] WANG W,MCKINNIE S M K,FARHAN M,et al.Angiotensin-Converting enzyme 2 metabolizes and partially inactivates pyr-apelin-13 and apelin-17[J].Hypertension,2016,68(2):365-377.
[4] DOU B X,LIU Y,LIU Y M,et al.Isolation and characterization of angiotensin I converting enzyme(ACE)inhibitory peptides from rice bran proteins and evaluation of activity and stability[J].Pakistan Journal of Zoology,2020,52(4):1 383-1 391.
[5] 魏冬.白酒糟中ACE抑制肽的鉴定与活性探究[D].无锡:江南大学,2020:3-5.WEI Dong.Identification and activity of ACE inhibitory peptides in distilled spent grain[D].Wuxi:Jiangnan University,2020:3-5.
[6] 李晶晶.扇贝裙边ACE抑制肽的分离纯化及活性研究[D].大连:大连海洋大学,2018:4.LI Jing-jing.Separation,purification and activity of ACE inhibitory peptides from scallop skirts[D].Dalian:Dalian Ocean University,2018:4.
[7] 何海艳,刘梦婷,杨爱萍,等.南瓜籽蛋白源ACE抑制肽的制备及其降血压活性[J/OL].食品科学.[2021-07-05].http://kns.cnki.net/kcms/detail/11.2206.TS.20210309.0909.002.html.HE Hai-yan,LIU Meng-ting,YANG Ai-ping,et al.Preparation of pumpkin seed protein ACE inhibitory peptide and its antihypertensive activity[J/OL].Food Science.[2021-07-05].http://kns.cnki.net/kcms/detail/11.2206.TS.20210309.0909.002.html.
[8] 封张萍,岳阳,刘东红,等.大米ACE抑制肽制备工艺优化和生物活性研究[J].食品科技,2021,46(2):210-217.FENG Zhang-ping,YUE Yang,LIU Dong-hong,et al.Optimization of preparation and bioactivity of rice ACE inhibitory peptides[J].Food Science and Technology,2021,46(2):210-217.
[9] LU X,SUN Q,ZHANG L X,et al.Dual-enzyme hydrolysis for preparation of ACE-inhibitory peptides from sesame seed protein:Optimization,separation,and identification[J].Journal of Food Biochemistry,2021,45(4):e13638.
[10] SUPAWEE P,PAPASSARA S,PIROONPORN S,et al.Angiotensin-I converting enzyme inhibitory peptide derived from the shiitake mushroom(Lentinula edodes)[J].Journal of Food Science and Technology,2021,58(1):85-97.
[11] 李楠楠,赵思明,张宾佳,等.稻米副产物的综合利用[J].中国粮油学报,2017,32(9):188-192.LI Nan-nan,ZHAO Si-ming,ZHANG Bin-jia,et al.Comprehensive utilization of rice byproducts[J].Journal of the Chinese Cereals and Oils Association,2017,32(9):188-192.
[12] FRATERRIGO GAROFALO S,TOMMASI T,FINO D.A short review of green extraction technologies for rice bran oil[J].Biomass Conversion and Biorefinery,2021,11(2):569-587.
[13] 谭书琪,张燕鹏,张维农,等.米糠蛋白加工利用研究进展[J].食品科技,2020,45(2):185-189.TAN Shu-qi,ZHANG Yan-peng,ZHANG Wei-nong,et al.Research progress in the processing and utilization of rice bran protein[J].Food Science and Technology,2020,45(2):185-189.
[14] 陈季旺,孙庆杰,姚惠源,等.利用米糠蛋白制备类阿片拮抗肽和降血压肽的研究[J].食品科技,2005(4):88-91.CHEN Ji-wang,SUN Qing-jie,YAO Hui-yuan,et al.Study on development of opioid antagonist peptides and antihypertensive peptides from rice bran protein[J].Food Science and Technology,2005(4):88-91.
[15] PHONGTHAI S,D'AMICO S,SCHOENLECHNER R,et al.Fractionation and antioxidant properties of rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion[J].Food Chemistry,2018,240:156.
[16] 樊金娟,付岩松,罗霞,等.米糠肽的抗氧化作用[J].食品与发酵工业,2009,35(12):78-81.FAN Jin-juan,FU Yan-song,LUO Xia,et al.Study on anti-oxidation effect of rice bran peptide[J].Food and Fermentation Industries,2009,35(12):78-81.
[17] 邹智鹏,王明洁,刘梦婷,等.小米米糠蛋白水解物及其膜分离组分的降血压相关活性研究[J].中国粮油学报,2020,35(6):31-38.ZOU Zhi-peng,WANG Ming-jie,LIU Meng-ting,et al.Antihypertensive properties of enzymatic hydrolysate of millet bran protein and its ultrafiltered fractions[J].Journal of the Chinese Cereals and Oils Association,2020,35(6):31-38.
[18] 张焱.米糠蛋白ACE抑制肽分离纯化工艺的研究[D].大庆:黑龙江八一农垦大学,2013:21.ZAHNG Yan.The separation and purification of rice bran protein peptide ACE[D].Daqing:Heilongjiang Bayi Agricultural University,2013:21.
[19] 骆琳,丁青芝,马海乐.96孔板法用于高通量血管紧张素转化酶抑制剂体外检测[J].分析化学,2012,40(1):129-134.LUO Lin,DING Qing-zhi,MA Hai-lei.Establishment of in vitro high-throughput activity detection method for angiotensin converting enzyme inhibitors based on 96 well plates[J].Chinese Journal of Analytical Chemistry,2012,40(1):129-134.
[20] 李莹.泥鳅蛋白源ACE抑制肽的酶法制备及其降压活性研究[D].南京:南京农业大学,2012:13-14.LI Ying.Preparation of angiotensin-i-converting enzyme inhibitory peptides by enzymolysis from loach(misgurnus anguillicaudatus)protein and its antihypertensive activities[D].Nanjing:Nanjing Agricultural University,2012:13-14.
[21] 陈秋銮,陈雪芹,马倩,等.酶解法制备牡丹籽ACE抑制肽及其稳定性[J].食品工业科技,2020,41(19):149-156.CHEN Qiu-luan,CHEN Xue-qin,MA Qian,et al.Preparation and stability of ACE inhibitory peptides from peony seed meal by enzymatic hydrolysis[J].Science and Technology of Food Industry,2020,41(19):149-156.
[22] 卫萍,游向荣,张雅媛,等.酶法制备火麻肽及其血管紧张素转化酶抑制活性研究[J].食品工业科技,2019,40(18):127-132.WEI Ping,YOU Xiang-rong,ZHANG Ya-yuan,et al.Preparation of cannabis sativa L.peptides by enzyme method and their activities of inhibiting angiotensin converting enzyme[J].Science and Technology of Food Industry,2019,40(18):127-132.
[23] YU Z P,WU S J,ZHAO W Z,et al.Identification of novel angiotensin I-converting enzyme inhibitory peptide from collagen hydrolysates and its molecular inhibitory mechanism[J].International Journal of Food Science & Technology,2020,55(9):3 145-3 152.
[24] YANG G L,QIN S,LI W J.Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins[J].Journal of Food Science,2021,86(6):2 457-2 467.
[25] 姚雨杉.鱿鱼加工副产物酶解、发酵工艺的优化和抗高血压肽的制备[D].广州:华南理工大学,2020:23.YAO Yu-shan.Optimization of enzymatic hydrolysis process and fermentation process of squid processing by-product and preparation of antihypertensive peptide[D].Guangzhou:South China University of Technology,2020:23.
[26] ISHAK N H,SHAIK M I,YELLAPU N K,et al.Purification,characterization and molecular docking study of angiotensin-I converting enzyme(ACE)inhibitory peptide from shortfin scad(Decapterus macrosoma)protein hydrolysate[J].Journal of Food Science and Technology,2021,58(12):4 567-4 577.
[27] CHEN Y H,LI J,DONG N G,et al.Separation and identification of ACE inhibitory peptides from defatted walnut meal[J].European Food Research and Technology,2020,246(10):2 029-2 038.