Isolation and identification of pathogenic fungi from postharvest blueberry in Sichuan province and the inhibition effect on them by using essential oil

Authors

LIU Chun-yan, College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316000 , China ；College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625000 , China
DING Jie, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625000 , China ；College of Food Science, Sichuan Tourism University, Chengdu, Sichuan 610100 , China
DENG Shang-gui, College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316000 , China
QIN Wen, College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625000 , China
LIU Yan, College of Food Science, Sichuan Tourism University, Chengdu, Sichuan 610100 , China

Abstract

Objective:This study aimed to provide strategy for controlling the fungal diseases of postharvest blueberry in Sichuan province.Methods:The pathogenic bacteria from 14 planting bases in Sichuan Province were isolate, identified, pathogenicity determination. Moreover, the effects of temperature and light on mycelia growth of the dominant and subdominant strains of blueberry after harvest were studied, and the effective natural antibacterial components against them were screened.Results:The blueberry postharvest rot diseases were caused by the following nine kinds of fungi:Aspergillus niger,Aspergillus tubingensis,Neofusicoccum parvum,Neopestalotiopsissp.,Fusarium graminearum,Alternaria alternata,Mucor fragilis,Penicilliumsp.,Cladosporium xylophilum.Aspergillus niger was the dominant species with the highest isolation frequency and the most pathogenic, andNeopestalotiopsissp.,Alternaria alternata,Penicilliumsp.,Cladosporium xylophilum were the subdominant species with isolation frequencies greater than 10%. The optimum growth temperature ranged from 20 ℃to 35 ℃of dominant and subdominant strains of blueberry postharvest. The effects of light on the mycelia growth ofA. niger，Neopestalotiopsissp., A. alternata,and C. xylophilum were significant，but that was insignificant on the growth ofPenicilliumsp.strains. Thymol showed good broad-spectrum antibacterial activity, the EC50 values of dominant and subdominant strains of blueberry postharvest was 30~50 mg/L, while the thymol applied directly to blueberry postharvest fungal control in aqueous solution should not be higher than 50 mg/L.Conclusion:Thymol is more effective on postharvest diseases of blueberry in Sichuan province.

Publication Date

7-20-2022

First Page

134

Last Page

140

DOI

10.13652/j.spjx.1003.5788.2022.90127

Recommended Citation

Chun-yan, LIU; Jie, DING; Shang-gui, DENG; Wen, QIN; and Yan, LIU (2022) "Isolation and identification of pathogenic fungi from postharvest blueberry in Sichuan province and the inhibition effect on them by using essential oil," Food and Machinery: Vol. 38: Iss. 4, Article 23.
DOI: 10.13652/j.spjx.1003.5788.2022.90127
Available at: https://www.ifoodmm.cn/journal/vol38/iss4/23

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