Study on preparation of collagen peptides from Micropterus salmoides skin and its antioxidant activity

Authors

HAN Meng-yao, National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou, Zhejiang 313000 , China ；Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Huzhou, Zhejiang 313000 , China ；Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, Huzhou, Zhejiang 313000 , China ；College of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000 , China
LI Xin-yue, National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou, Zhejiang 313000 , China ；Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Huzhou, Zhejiang 313000 , China ；Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, Huzhou, Zhejiang 313000 , China ；College of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000 , China
WANG Xiao-mei, National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou, Zhejiang 313000 , China ；Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Huzhou, Zhejiang 313000 , China ；Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, Huzhou, Zhejiang 313000 , China ；College of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000 , China
WANG Guo-zhi, National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou, Zhejiang 313000 , China ；Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Huzhou, Zhejiang 313000 , China ；Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, Huzhou, Zhejiang 313000 , China ；College of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000 , China
YE Jin-yun, National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou, Zhejiang 313000 , China ；Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Huzhou, Zhejiang 313000 , China ；Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, Huzhou, Zhejiang 313000 , China ；College of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000 , China
ZHANG Zhong-shan, National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou, Zhejiang 313000 , China ；Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Huzhou, Zhejiang 313000 , China ；Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, Huzhou, Zhejiang 313000 , China ；College of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000 , China

Abstract

Objective:Collagen was extracted from the skin of Micropterus salmoides and used to explore the effect of enzyme types on the antioxidant activity of collagen peptides.Methods:Four kinds of enzymes were used, namely alkaline protease, neutral protease, papain and trypsin. The enzymatic hydrolysis of collagen Alkaline protease enzymatically hydrolyze collagen peptide (APP), Neutral protease enzymatically hydrolyze collagen peptide (NPP), Papain enzymatically hydrolyze collagen peptide(PP), Trypsin hydrolysis of collagen peptide(TP), were obtained and compared their total antioxidant activity, including reducing activity, DPPH, ·OH and O-2·free radical scavenging ability, ferrous ion chelating ability and lipid Oxidation inhibitory activity.Results:Four kinds of proteases enzymatically hydrolyze collagen peptides all have antioxidant activity, and the antioxidant capacity was positively correlated with the concentration of collagen peptides. Among them, APP has the best antioxidant activity. The total antioxidant activity and reducing activity respectively were 1.725 and 0.958, and the scavenging rates for DPPH and O-2·radical respectively were 56.35% and 38.41%, respectively.Conclusion:The collagen peptides hydrolyzed by alkaline protease have better antioxidant activity.

Publication Date

7-20-2022

First Page

175

Last Page

182

DOI

10.13652/j.spjx.1003.5788.2022.90026

Recommended Citation

Meng-yao, HAN; Xin-yue, LI; Xiao-mei, WANG; Guo-zhi, WANG; Jin-yun, YE; and Zhong-shan, ZHANG (2022) "Study on preparation of collagen peptides from Micropterus salmoides skin and its antioxidant activity," Food and Machinery: Vol. 38: Iss. 4, Article 29.
DOI: 10.13652/j.spjx.1003.5788.2022.90026
Available at: https://www.ifoodmm.cn/journal/vol38/iss4/29

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