Abstract
Objective: This study aimed to investigate the protective effect and mechanism of solid beverage with vine tea combined with and cassia on alcoholic liver injury in rats. Methods: SD rats were given 43%vol aging by gavage to establish model of alcoholic liver injury. The model was fed with low, medium and high doses of solid beverage. The appropriate ratio of solid beverages was detected by cell test, the physiological and biochemical indicators of rats were detected by using kits, and the expression of related differential genes was studied by transcriptomic analysis to assessing the effect of vine tea extract combined with cassia extract solid beverage on alcoholic liver injury in rats and revealing the mechanism of action. Results: Compared with the model group, the activities of ALT, AST and LDH in the solid beverage group were significantly decreased; the levels of IL-6 and TNF-α was significantly reduced; the activities of GSH and SOD in the liver were significantly increased, while the contents of MDA and TG were significantly decreased; finally the oxidative stress and inflammatory response in rat liver were significantly improved. Compared to the model group, the inflammatory cell infiltration and vacuolar degeneration of liver cells in the liver pathological sections of the solid beverage group were significantly reduced; transcriptomic analysis speculated that the regulation of genes such as spermidine synthase, Sirt7, RT1-A2, RT1-CE1 and other pathways and amino acid metabolism are related to vine tea extract combined with cassia extract solid beverage to alleviate alcoholic liver injury. Conclusion: Vine tea extract combined with cassia extract solid beverage can alleviate alcohol-induced liver injury by improving liver function, reducing liver inflammation and oxidative stress.
Publication Date
6-30-2022
First Page
157
Last Page
165,172
DOI
10.13652/j.spjx.1003.5788.2022.90177
Recommended Citation
Li, ZHAN; Quan-quan, TANG; Zi-ying, ZHANG; Yu, SHI; Jian-zao, TENG; Zi-long, LIU; Zhong-hua, LIU; and Sheng, ZHANG
(2022)
"Protective effect of vine tea extract combined with cassia extract on chronic alcoholic liver injury in rats,"
Food and Machinery: Vol. 38:
Iss.
5, Article 29.
DOI: 10.13652/j.spjx.1003.5788.2022.90177
Available at:
https://www.ifoodmm.cn/journal/vol38/iss5/29
References
[1] 杨冬晗,张文龙,侯瑞丽,等.Toll样受体4信号途径在酒精性肝损伤中的作用研究进展[J].中国比较医学杂志,2021,31(10):126-130.YANG Dong-han,ZHANG Wen-long,HOU Rui-li,et al.The role of the toll-like receptor 4 signaling pathway in alcoholic liver injury[J].Chin J Comp Med,2021,31(10):126-130.
[2] 颜蜜,滕春丽,陶会,等.红托竹荪多糖对大鼠酒精性肝损伤的保护作用[J].菌物学报,2022,41(2):1-18.YAN Mi,TENG Chun-li,TAO Hui,et al.Protective effects of Dictyophora rubrovalvata polysaccharide on alcoholic liver injury in rats[J].Mycosystema,2022,41(2):1-18.
[3] 欧阳香,程虹毓,胡伟琼.黄酮类化合物抗酒精性肝损伤作用及机制研究进展[J].中国药理学通报,2020,36(9):1 200-1 205.OU Yang-xiang,CHENG Hong-yu,HU Wei-qiong.Research progress on the effect and mechanism of flavonoids on alcoholic liver injury[J].Chinese Pharmacological Bulletin,2020,36(9):1 200-1 205.
[4] 邓杰夫,邓武成,胡维军,等.张家界市莓茶园杂草调查研究初报[J].安徽农学通报,2021,27(17):79-81.DENG Jie-fu,DENG Wu-cheng,HU Wei-jun,et al.Preliminary investigation on weeds in vine tea garden in Zhangjiajie city[J].Anhui Agri Sci Bull,2021,27(17):79-81.
[5] 张云坤,娟李,丹黄,等.中药藤茶化学成分及抗感染作用研究进展[J].世界科学技术-中医药现代化,2021,23(6):2 012-2 022.ZHANG Yun-kun,LI Juan,HUANG Dan,et al.Research progress on chemical constituents and anti-infective effects of the traditional chinese medicine vine tea(ampelopsis grossedentata)[J].Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2021,23(6):2 012-2 022.
[6] HOU X L,TONG Q,WANG W Q,et al.Suppression of inflammatory responsesby dihydromyricetin,a flavonoid from ampelopsis grossedentata,via inhibiting the activation of NF-kappaB and MAPK signaling pathways[J].J Nat Prod,2015,78(7):1 689-1 696.
[7] WU Y,BAI J,ZHONG K,et al.A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus[J].Food Chem,2017,218:463-470.
[8] YAN L F,MEI C S,BING Z H.Investigation of in vitro antioxidant activity of dihydromyricetin and flavonoids rich extract from vine tea(Ampelopsis grossedentata)[J].Traditional Medicine Research,2021,6(1):1-11.
[9] ZHOU X,YU L,ZHOU M,et al.Dihydromyricetin ameliorates liver fibrosis via inhibition of hepatic stellate cells by inducing autophagy and natural killer cell-mediated killing effect[J].Nutr Metab(Lond),2021,18(1):64.
[10] 蔡建峰,蓝文明,魏亮龙,等.显齿蛇葡萄总黄酮对急性酒精中毒小鼠的解酒及肝损伤的保护作用[J].福建中医药,2016,47(3):16-17.CAI Jian-feng,LAN Wen-ming,WEI Liang-long,et al.The protective effect of total flavonoids from Ampelopsis grossedentata on hangover and liver injury in mice with acute alcoholism[J].Fujian Journal of Traditional Chinese Medicine,2016,47(3):16-17.
[11] 刘金金,殷军艺,黄晓君,等.决明子多糖结构和生物活性功能研究进展[J].食品研究与开发,2019,40(23):212-221.LIU Jin-jin,YIN Jun-yi,HUANG Xiao-jun,et al.Research progress on structure and biological activity of polysaccharide from Cassia seed[J].Food Research and Development,2019,40(23):212-221.
[12] HOU J,GU Y,ZHAO S,et al.Anti-inflammatory effects of aurantio-obtusin from seed of Cassia obtusifolia L.through modulation of the NF-κB pathway[J].Molecules,2018,23(12):3 093.
[13] PAUDEL P,JUNG H A,CHOI J S.Anthraquinone and naphthopyrone glycosides from Cassia obtusifolia seeds mediate hepatoprotection via Nrf2-mediated HO-1 activation and MAPK modulation[J].Arch Pharm Res,2018,41(6):677-689.
[14] 郑淑娟,谢子鑫,方靖靖,等.橄榄苦苷改善db/db小鼠糖尿病的肝脏转录组学及生物信息学分析[J/OL].食品科学.(2021-11-17)[2022-02-07].https://www.spkx.net.cn/CN/article/advancedSearch.do.ZHENG Shu-juan,XIE Zi-xin,FANG Jing-jing,et al.Hepatic transcriptomics and bioinformatics analysis of oleuropein alleviating diabetes in db/db mice[J/OL].Food Science.(2021-11-17)[2022-02-07].https://www.spkx.net.cn/CN/article/advancedSearch.do.
[15] 牟伊,宋雨,文帅,等.Nrf2抑制剂在肿瘤治疗中的研究进展[J].药学学报,2022,57(2):321-330.MOU Yi,SONG Yu,WEN Shuai,et al.Research progress of Nrf2 inhibitor in tumor ther[J].Acta Pharmaceutica Sinica,2022,57(2):321-330.
[16] ROJO DE LA VEGA M,CHAPMAN E,ZHANG D D.NRF2 and the Hallmarks of Cancer[J].Cancer Cell,2018,34(1):21-43.
[17] YANG J,ZHU A,XIAO S,et al.Anthraquinones in the aqueous extract of Cassiae semen cause liver injury in rats through lipid metabolism disorder[J].Phytomedicine,2019,64:153059.
[18] 江颖倩,彭梦超,吴建国,等.长片金线兰多糖对四氯化碳诱导急性肝损伤小鼠的保护作用[J].中草药,2021,52(19):5 932-5 938.JIANG Ying-qian,PENG Meng-chao,WU Jian-guo,et al.Protective effect of polysaccharide fromAnoectochilus longilobus on CCl4-induced acute liver injury in mice[J].Chinese Traditional and Herbal Drugs,2021,52(19):5 932-5 938.
[19] 谭丽娟,徐沛演,俐李.血清IL-6、TNF-α检测对药物性肝损伤严重程度的预测价值研究[J].中国当代医药,2021,28(10):71-74.TAN Li-juan,XU Pei-yan,LI Li.Study on the predictive value of IL-6 and TNF-α in the severity of drug-induced liver injury[J].China Modern Medicine,2021,28(10):71-74.
[20] GANESAN K,JAYACHANDRAN M,XU B.A critical review on hepatoprotective effects of bioactive food components[J].Crit Rev Food Sci Nutr,2018,58(7):1 165-1 229.