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Abstract

Objective: Improving the accuracy of the determination of fat in CMC-Na fermented milk. Methods: The national standard method-alkali hydrolysis method was used to determine the fat contained in CMC-Na fermented milk. By optimizing the key parameters in the alkali hydrolysis method, hydrolysis of hydrolysis instead of ammonia hydrolysis, and adding metal ions to the alkali hydrolysis method, the suitable fat containing CMC-Na fermented milk was explored. Results: ① With the increase of CMC-Na content, the lower the fat result in CMC-Na fermented milk measured by alkaline hydrolysis; ② The change of the four key conditions, such as the ammonia volume, hydrolysis time, hydrolysis temperature and extraction frequency in alkaline hydrolysis, will not affect the determination on fat in CMC-Na fermented milk. ③ When 2 mL hydrochloric acid hydrolysis was used to instead ammonia hydrolysis, the fat determination results in fermented milk with different CMC-Na content (0.50%, 0.75%, 1.00%) were almost the same as the alkali hydrolysis method for the determination of CMC-Na-free fermented milk; ④ When metal ions Na+, K+ and Ca2+ were added to the alkali hydrolysis method, the higher the concentration of Na+ and K+, the higher the fat determination results containing CMC-Na fermented milk, and the normal value will be achieved after the concentration of metal ions reaches to 0.5 mol/L, while the increase of Ca2+ concentration, and the fat determination will be determined. As a result, it increased slightly first, and then decreased significantly. Conclusion: The addition of CMC-Na will make the fat determination results in the fermented milk low. Hydrochloric acid hydrolysis or Na+ and K+ can be added to the alkali hydrolysis method to improve the accuracy of fat determination in the fermented milk containing CMC-Na.

Publication Date

6-30-2022

First Page

37

Last Page

42,86

DOI

10.13652/j.spjx.1003.5788.2022.60029

References

[1] BOURRIE B,WILLING B P,COTTER P D.The microbiota and health promoting characteristics of the fermented beverage kefir[J].Front Microbiol,2016,7:647.
[2] 侯彩云,郭秀兰,彭家宣,等.芦丁对低脂酸奶品质和抗氧化能力的影响[J].食品与机械,2019,35(4):37-41.HOU Cai-yun,GUO Xiu-lan,PENG Jia-xuan,et al.Effect of supplementing rutin on quality and antioxidant capacity of low-fat yoghurt[J].Food & Machinery,2019,35(4):37-41.
[3] GARCÍA-BURGOS M,MORENO-FERNÁNDEZ J,ALFÉREZ M J M,et al.New perspectives in fermented dairy products and their health relevance[J].Jouanal of Functional Foods,2020,72:104059.
[4] TREMBLAY A,PANAHI S.Yogurt consumption as a signature of a healthy diet and lifestyle[J].The Journal of Nutrition,2017,147(7):1 476S-1 480S.
[5] 张宇.中国酸奶零售市场持续增长酸奶系列产品受消费者追捧[N].中国食品安全报,2015-04-09(B01).ZHANG Yu.China's yoghurt retail market continues to grow,and yoghurt series products are sought after by consumers[N].China Food Safety News,2015-04-09(B01).
[6] UNGER A,TORRES-GONZALEZ M,KRAFT J.Dairy fat consumption and the risk of metabolic syndrome:An examination of the saturated fatty acids in dairy[J].Nutrients,2019,11(9):2 200.
[7] 孟婷婷,周星,陆振猷,等.脂肪对低脂植物蛋白饮料风味及体系稳定性的影响研究进展[J].食品与机械,2019,35(7):220-225.MENG Ting-ting,ZHOU Xing,LU Zhen-you,et al.Reviews of effects of fat on the flavor and system stability of low-fat plant protein beverage[J].Food & Machinery,2019,35(7):220-225.
[8] 姜铁民,贺小龙,高一依,等.不同酸奶脂肪营养价值的评价及其影响因素[J].中国食品添加剂,2022,33(1):154-163.JIANG Tie-min,HE Xiao-long,GAO Yi-yi,et al.Nutritional value of fat in yogurt and its influencing factors[J].China Food Additives,2022,33(1):154-163.
[9] 孙雷.利用近红外光谱法检测酸奶中蛋白质和脂肪含量[D].长春:吉林大学,2008:4-6.SUN Lei.Using near-infrared spectroscopy to detect the content of protein and fat in yoghourt[D].Changchun:Jilin University,2008:4-6.
[10] 朱彦群.灭菌调味乳及酸牛乳中脂肪的快速测定[J].乳业科学与技术,2004(30):111.ZHU Yan-qun.Fast detecting fat content in sterilized milk and yoghurt[J].Journal of Dairy Science and Technology,2004(30):111.
[11] BURGARDT V C F,ZUGE L C B,DE BONNA Sartor G,et al.The addition of carboxymethylcellulose in caseinomacropeptide acid gels:Rheological,optical and microstructural characteristics[J].Food Hydrocolloids,2015,11(2):1-11.
[12] 陈一萌,李思宁,唐善虎,等.不同稳定剂对凝固型牦牛酸奶在冷藏后熟过程中品质及风味的影响[J].食品工业科技,2018,39(4):188-196.CHEN Yi-meng,LI Si-ning,TANG Shan-hu,et al.Effect of different stabilizers on the quality and flavors of set-style yak yoghurt during post-fermentation storage[J].Science and Technology of Food Industry,2018,39(4):188-196.
[13] FUQUAY J W,HERR B.Additives in dairy foods:Types and functions of additives in dairy products encyclopedia of dairy sciences[M].2nd ed.San Diego:Academic Press,2011:34-40.
[14] 宋艳梅,刘玉英,夏忠悦,等.酸奶中脂肪含量检测差异影响因素的研究[J].食品安全质量检测学报,2021,12(6):2 146-2 152.SONG Yan-mei,LIU Yu-ying,XIA Zhong-yue,et al.Study on the factors influencing the detection difference of fat content in yogurt[J].Journal of Food Safety and Quality,2021,12(6):2 146-2 152.
[15] KÄSNER U.Structure and solution properties of sodium carboxymethyl cellulose[J].Colloids and Surfaces A,1997,123:307-328.
[16] DU B Q,LI J,ZHANG H B,et al.Influence of molecular weight and degree of substitution of Carboxymethyl cellulose on the stability of acidified milk drinks[J].Food Hydrocolloids,2009,23(5):1 420-1 426.
[17] 晏凯,刘晓彤,刘悦,等.碱水解法测定乳及乳制品中脂肪的含量[J].食品安全质量检测学报,2020,11(1):82-85.YAN Kai,LIU Xiao-tong,LIU Yue,et al.Determination of fat content in milk and dairy products by alkaline hydrolysis[J].Journal of Food Safety and Quality,2020,11(1):82-85.
[18] 邱蓉.CMC和大豆多糖对酸性乳饮料中酪蛋白稳定机理的研究[D].杭州:浙江工商大学,2010.QIU Rong.Casein in the acidified milk drinks induced by CMC and soybean polysaccharide[D].Hangzhou:Zhejiang Gongshang University,2010.
[19] 吴晓静.碱水解法测定乳粉中脂肪含量[J].食品安全导刊,2018(5):63-64.WU Xiao-jing.Determination of fat content in milk powder by alkaline hydrolysis[J].China Food Safety,2018(5):63-64.
[20] 刘祥.高粘度羧甲基纤维素钠的研制[J].安徽工业大学学报,2001(2):114-116.LIU Xiang.Preparation of carboxymethyl cellulose with high viscosity[J].Journal of Anhui University of Technology,2001,18(2):114-116.
[21] MITSUMATA T.pH-respose of chitosan,K-carrageenan,carboxymethylcellulose sodium saltcomplex hydrogels[J].Polymer,2003,44:7 103-7 111.
[22] 禚林娜,刘石雪,汪凤玲,等.酸水解法测定脂肪的研究及方法优化[J].现代食品,2020(21):180-183.ZHUO Lin-na,LIU Shi-xue,WANG Feng-ling,et al.Study on the determination of fat by acid hydrolysis method and its optimization[J].Modern Food,2020(21):180-183.
[23] 何曼君,陈维孝,董西侠.高分子物理[M].上海:复旦大学出版社,2001:137-139.HE Man-jun,CHEN Wei-xiao,DONG Xi-xiang.Polymer physics[M].Shanghai:Fudan University Press,2001:137-139.
[24] HEMAR Y,HORNE D S.Electrostatic interactions in adsorbed protein layers probed by a sedimentation technique[J].Journal of Colloid and Interface Science,1998,206(1):138-145.
[25] ISABELLE B,SERGE P.Molecular basis of Ca2+-induced gelation in alginates andpectins:The egg-box model recisited[J].Biomacromolecules,2001,2:1 089-1 096.
[26] 杜柏桥.羧甲基纤维素钠(CMC)与酪蛋白的相互作用及其稳定酸性乳体系机理的研究[D].上海:上海交通大学,2008:47-52.DU Bo-qiao.The interaction between carboxymethylcellulose(CMC)and casein micelle and the stabilization mechanism of acidified milk drinks induced by CMC[D].Shanghai:Shanghai Jiao Tong University,2008:47-52.

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