Abstract
Objective:The purpose of this study was to explore the antioxidative activity in vitro experiments and the anti-fatigue activity of chickpea oligopeptides in mice. Methods:In this study, the antioxidant capacity of chickpea oligopeptide was detected by in vitro method, and the anti-fatigue capacity of chickpea oligopeptide was comprehensively evaluated by detecting physiological and biochemical indexes of mice. Results:The superoxide anion radical scavenging ability IC50of chickpea oligopeptide was 4.26 mg/mL, which was significantly higher than that of GSH positive control group (P<0.05); The reducing power of chickpea oligopeptide was significantly higher than that of GSH positive control (P<0.05). In addition, at low, medium and high doses, the fatigue tolerance, muscle glycogen and liver glycogen reserve capacity of mice were improved, and the concentration of serum urea nitrogen, malondialdehyde and lactic acid was decreased (P<0.05). It can significantly increase the activities of catalase, superoxide dismutase and glutathione peroxidase (P<0.05). Conclusion:Chickpea oligopeptides have significant anti-fatigue effects.
Publication Date
9-10-2022
First Page
148
Last Page
152
DOI
10.13652/j.spjx.1003.5788.2022.80007
Recommended Citation
Qian-sheng, Jia; Yuan-yang, Liu; and Dan, Li
(2022)
"Study on anti-fatigue activities of chick-pea oligopeptides,"
Food and Machinery: Vol. 38:
Iss.
6, Article 26.
DOI: 10.13652/j.spjx.1003.5788.2022.80007
Available at:
https://www.ifoodmm.cn/journal/vol38/iss6/26
References
[1] 张涛,江波,王璋,等.鹰嘴豆的营养价值及其应用[J].粮食与油脂,2004(7):18-20.ZHANG Tao,JIANG Bo,WANG Zhang,et al.Nutritional value and application of chickpea[J].Cereals and Oils,2004(7):18-20.
[2] 刘宇,刘春泉,庄世文,等.鹰嘴豆肽清除自由基作用的研究[J].食品科技,2009(3):173-176.LIU Yu,LIU Chun-quan,ZHUANG Shi-wen,et al.Study on radical-scavenging effect of chickpea polypeptide[J].Food Science and Technology,2009(3):173-176.
[3] WEN S,TAO H,DGA B,et al.Evaluation of hypolipidemic peptide(Val-Phe-Val-Arg-Asn)virtual screened from chickpea peptides by pharmacophore model in high-fat diet-induced obese rat[J].Journal of Functional Foods,2019,54:136-145.
[4] 李睿珺,秦勇,周雅琳,等.鹰嘴豆肽对免疫低下小鼠免疫功能的影响[J].食品科学,2020,41(21):133-139.LI Rui-jun,QIN Yong,ZHOU Ya-lin,et al.Effect of chickpea peptide on immune function of immunocompromised mice[J].Food Science,2020,41(21):133-139.
[5] 姚梦,王晓蕾,申其淇,等.运动性心理疲劳影响因素的研究进展与展望[J].体育时空,2015(12):161.YAO Meng,WANG Xiao-lei,SHEN Qi-qi,et al.Research progress and prospect of influencing factors of sports psychological fatigue[J].Sports Time and Space,2015(12):161.
[6] NIEHUES M,EULER M,GEORGI G,et al.Peptides from Pisum sativum L.enzymatic protein digest with anti-adhesive activity against Helicobacter pylori:Structure-activity and inhibitory activity against BabA,SabA,HpaA and a fibronectin-binding adhesin[J].Molecular Nutrition & Food Research,2010,54(12):1 851-1 861.
[7] NDIAYE F,VUONG T,DUARTE J,et al.Anti-oxidant,anti-inflammatory and immunomodulating properties of an enzymatic protein hydrolysate from yellow field pea seeds[J].European Journal of Nutrition,2012,51(1):29-37.
[8] 闫萍.大豆小分子多肽的抗疲劳作用及其机制研究[D].吉林:吉林大学,2008:4-7.YAN Ping.Study on anti fatigue effect and mechanism of soybean small molecular peptides[D].Jilin:Jilin University,2008:4-7.
[9] 王雨晴,毕园,张瑞雪,等.豌豆低聚肽中具有发挥功能活性潜力的结构研究[J].食品与发酵工业,2020,46(17):54-59.WANG Yu-qing,BI Yuan,ZHANG Rui-xue,et al.Study on the structure of pea oligopeptides with potential function and activity[J].Food and Fermentation Industry,2020,46(17):54-59.
[10] WU R B,WU C L,LIU D,et al.Antioxidant and anti-freezing peptides from salmon collagen hydrolysate prepared by bacterial extracellular protease[J].Food Chemistry,2018,248:346-352.
[11] AHN C B,KIM J G,JE J Y.Purification and antioxidant properties of octapeptide from salmon byproductprotein hydrolysate by gastrointestinal digestion[J].Food Chemistry,2014,147:78-83.
[12] KOVACS-NOLAN J,ZHANG H,IBUKI M,et al.The Pep T1-transportable soy tripeptide VPY reduces intestinal inflammation[J].Biochimica et Biophysica Acta,2012,1 820(11):1 753-1 763.
[13] WEI L,WEN Y T,LEE M C,et al.Effects of isolated soy protein and strength exercise training on exercise performance and biochemical profile in postpartum mice[J].Metabolism,2019(94):18-27.
[14] 刘娜,李湘浓,吴翱,等.大豆寡肽抗疲劳作用的实验研究[J].中国实验诊断学,2010,14(2):201-203.LIU Na,LI Xiang-nong,WU Ao,et al.Experimental study on anti fatigue effect of soybean oligopeptides[J].Chinese Journal of Experimental Diagnostics,2010,14(2):201-203.
[15] 张铁华,殷涌光,刘静波,等.高F值寡肽抗疲劳作用的研究及其饮料的开发[J].食品科学,2007(5):308-312.ZHANG Tie-hua,YIN Yong-guang,LIU Jing-bo,et al.Study on anti fatigue effect of high f-value oligopeptide and development of its beverage[J].Food Science,2007(5):308-312.
[16] HSU W H,QIU W L,TSAO S M,et al.Effects of WSG,a polysaccharide from Ganoderma lucidum,on suppressing cell growth and mobility of lung cancer[J].International Journal of Biological Macromolecules,2020(165):1 604-1 613.
[17] YANG Q Y,LAI X D,OUYANG J,et al.Effects of Ginsenoside Rg3 on fatigue resistance and SIRT1 in aged rats[J].Toxicology,2018(409):144-151.
[18] SURHIO M M,WANG Y,FANG S,et al.Anti-fatigue activity of a Lachnum polysaccharide and its carboxymethylated derivative in mice[J].Bioorganic & Medicinal Chemistry Letters,2017,27(20):4 777-4 780.
[19] YEH T S,HUANG C C,CHUANG H L,et al.Angelica sinensis improves exercise performance and protects against physical fatigue in trained mice[J].Molecules,2014,19(4):3 926-3 939.
[20] XI X,GUO S,GUO H,et al.Anti-exercise-fatigue and promotion of sexual interest activity of total flavonoids from wasps drone-pupae in male mice[J].Biomedicine & Pharmacotherapy,2018(107):254-261.
[21] CHI A,LI H,KANG C,et al.Anti-fatigue activity of a novel polysaccharide conjugates from Ziyang green tea[J].International Journal of Biological Macromolecules,2015(80):566-572.
[22] LUO C,XU X,WEI X,et al.Natural medicines for the treatment of fatigue:Bioactive components,pharmacology,and mechanisms[J].Pharmacological Research,2019(148):104 409.