Abstract
Objective:This study focused on improving comprehensive utilization rate of ziziphi spinosae semen resources. Methods:With the ultrasonic power, ultrasonic time, solid-liquid ratio, ethanol volume fraction and ultrasonic temperature as the influencing factors, and the extraction rate of isovitexin-glucopyranoside in ziziphi spinosae semen as the index, the extraction process parameters of isovitexin-glucopyranoside were optimized by response surface methodology. Results: The optimal extraction conditions of isovitexin-glucopyranoside were ultrasonic power 405 W, ultrasonic time 60 min, solid-liquid ratio 1∶16 (g/mL) and ethanol volume fraction 75%. Under the control of these conditions, the extraction rate of isovitexin-glucopyranoside was 0.705%, which was close to the predicted value (0.719%), indicating that the predicted value of the extraction rate of isovitexin-glucopyranoside fit well with the actual situation. Conclusion: The ultrasonic assisted extraction of isovitexin-glucopyranoside is stable and reliable with high extraction rate.
Publication Date
9-14-2022
First Page
170
Last Page
176
DOI
10.13652/j.spjx.1003.5788.2022.90193
Recommended Citation
Li, Gong; Ying, Liu; Ting-ting, Yang; Qing, Wang; and Yan-qing, Zhang
(2022)
"Optimization of ultrasound-assisted extraction of isovitexin-glucopyranoside from ziziphi spinosae semen seeds,"
Food and Machinery: Vol. 38:
Iss.
6, Article 30.
DOI: 10.13652/j.spjx.1003.5788.2022.90193
Available at:
https://www.ifoodmm.cn/journal/vol38/iss6/30
References
[1] 贾春伶,王锦燕,赵奎君,等.《本草纲目》木部药食同源药用植物的记载及其启示[J].中国现代中药,2021,23(6):1 094-1 102.JIA Chun-ling,WANG Jin-yan,ZHAO Kui-jun,et al.Records of medicinaland edible plants in the wood secation of compendium of meteria medica and relevation[J].Modern Chinese Medicine,2021,23(6):1 094-1 102.
[2] 韩荣欣,张红印,周光鑫,等.体外模拟消化对酸枣仁蛋白酶解产物抗氧化活性的影响[J].食品与机械,2021,37(7):171-176.HAN Rong-xin,ZHANG Hong-yin,ZHOU Guang-xin,et al.Effect of simulated gastrointestinal disgestion in vitro o the antioxidant activity of semen ziziphi spinosae protein hydrolysates[J].Food & Machinery,2021,37(7):171-176.
[3] 国家药典委员会.中华人民共和国药典[S].2020版.北京:中国医药科技出版社,2020:382-383.National Commission of Chinese Pharmacopoeia.Pharmacopoeia of the People's Republic of China[S].2020 ed.Beijing:China MedicalScience,2020:382-383.
[4] 宫丽,解军波,赖长江生,等.酸枣仁及其副产物综合利用的研究进展[J].中国实验方剂学杂志,2021,27(3):222-230.GONG Li,XIE Jun-bo,LAI Chang-jiang-sheng,et al.Research progress of comprehensive utilization of Ziziphi Spinosae Semen and its by-products[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(3):222-230.
[5] WANG Yi-fang,HUANG Mei,LU Xin-yi,et al.Ziziphi spinosae lily powder suspension in the treatment of depression-like behaviors in rats[J].BMC Complementary and Alternative Medicine,2017,17:238.
[6] XU Fan-xing,HE Bo-sai,XIAO Feng,et al.Neuroprotective effects of spinosin on recovery of learning and memory in a mouse model of Alzheimerʼs disease[J].Biomolecules & Therapeutics,2019,27(1):71-77.
[7] 黄之镨,马伟光.酸枣仁及活性物质的药理研究进展[J].中国民族民间医药,2018,27(3):57-60.HUANG Zhi-pu,MA Wei-guang.Research progress in the pharmacological of Semen Ziziphi Spinosae and its active ingredients[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2018,27(3):57-60.
[8] JIANG Jian-guo,HUANG Xiao-juan,CHEN Jian,et al.Comparison of the sedative and hypnotic effects of flavonoids,saponins,and polysaccharides extracted from Semen Ziziphus jujube[J].Natural Product Research,2007,21(4):310-320.
[9] JUNG In-ho,LEE Hyung,PARK Se-jin,et al.Ameliorating effect of spinosin,a C-glycoside flavonoid,on scopolamine induced memory impairment in mice[J].Pharmacology Biochemistry and Behavior,2014,120:88-94.
[10] LEE Hyung-eun,JEON Se-jin,RYU Byeol,et al.Swertisin,a C-glucosylflavone,ameliorates scopolamine-induced memory impairment in mice with its adenosine A1 receptor antagonistic property[J].Behavioural Brain Research,2016,306:137-145.
[11] LI Hong-juan,SHI Ji-ling,LI Yuan-yuan,et al.Purification of spinosin from Ziziphi Spinosae Semen using macroporous resins followed by preparative high-performance liquid chromatography[J].Journal of Separation Science,2019,42(19):3 134-3 140.
[12] VILLANUEVA Javier-rodriguez,VILLANUEVA Laura-rodriguez.Experimental and clinical pharmacology of Ziziphus jujube Mills[J].Phytotherapy Research,2017,31(3):347-365.
[13] WANG L E,CUI X Y,CUI S Y,et al.Potentiating effect of spinosin,a C-glycoside flavonoid of Semen Ziziphi spinosae,on pentobarbital-induced sleep may be related to postsynaptic 5-HT1A receptors[J].Phytomedicine,2010,17(6):404-409.
[14] LEE Young-hwan,JEON Se-jeon,LEE Hyung-eun,et al.Spinosin,a C-glycoside avonoid,enhances cognitive performance and adult hippocampal neurogenesis in mice[J].Pharmacology Biochemistry and Behavior,2016,145:9-16.
[15] 张婷,张岩,王文彤,等.酸枣仁中黄酮成分及其药理作用研究进展[J].天津药学,2018(2):69-74.ZHANG Ting,ZHANG Yan,WANG Wen-tong,et al.Researchprogress on flavonoids and pharmacological action in Semen Ziziphi Spinosae[J].Tianjin Pharmacy,2018(2):69-74.
[16] 张扬.铁皮石斛叶水溶性成分分离及活性筛选的研究[D].广州:广州中医药大学,2016:39-43.ZHANG Yang.Research on the isolation and activity of water-soluble components from dendrobium offcinale leaves[D].Guangzhou:Guangzhou University of Chinese Medicine,2016:39-43.
[17] 李亚欣.酸枣仁黄酮的制备及其在N1E-115细胞中的抗氧化应激作用研究[D].天津:天津商业大学,2017:25-32.LI Ya-xin.Preparation of flavonoids from Semen Ziziphi Spinosae and its antioxidative stress effect in NqE-115 cells[D].Tianjin:Tianjin University of Commerce,2017:25-32.
[18] 宋盼盼.酸枣仁黄酮跨膜转运及其磷脂复合物自微乳制剂研究[D].天津:天津商业大学,2018:26-38.SONG Pan-pan.Study ontransportation characteristics of flavonoids from Ziziphi Spinosae Semen and its self-microemulsion of phospholipid complex[D].Tianjin:Tianjin University of Commerce,2018:26-38.
[19] 赖毅勤,周宏兵.近年来黄酮类化合物提取和分离方法研究进展[J].食品与药品,2007,9(4):54-58.LAI Yi-qin,ZHOU Hong-bing.Research advance on extraction and separation technology of flavonoids[J].Food and Drug,2007,9(4):54-58.
[20] 丁轲,谷禹,陆晶晶,等.酸枣仁黄酮组分提取纯化工艺的研究[J].中国食品学报,2011,11(4):62-70.DING Ke,GU Yu,LU Jing-jing,et al.Study on extraction and purifition technology of flavones from Ziziphi Spinose Semen[J].Journal of Chinese Institute of Food Science and Technology,2011,11(4):62-70.
[21] 邬方宁.超声提取技术在现代中药中的应用[J].中草药,2007,38(2):315-316.WU Fang-ning.Application of ultrasonic extraction technology in modern traditional Chinese medicine[J].Chinese Traditional and Herbal Drugs,2007,38(2):315-316.
[22] 唐婷范,黄芳丽,朱家庆,等.超声波辅助提取葛根异黄酮的工艺优化及其抑菌活性研究[J].食品研究与开发,2020,41(12):30-36.TANG Ting-fang,HUANG Fang-li,ZHU Jia-qing,et al.Optimization of extraction of isoflavones from pueraria lobata by ultrasonic-assisted and antibacterial activity[J].Food Research and Development,2020,41(12):30-36.
[23] 周海旭,谢美玉,高晗,等.樟树叶木脂素和多酚超声辅助同步提取工艺优化[J].食品与机械,2020,36(10):143-148.ZHOU Hai-xu,XIE Mei-yu,GAO Han,et al.Optimization of extraction process of lignans and polyphenols by ultrasonic wave assisted extraction from Cinnamomum camphora[J].Food & Machinery,2020,36(10):143-148.
[24] 王李平,刘小琴,范华均,等.响应面法优化—微波辅助萃取—高效液相色谱法测定农吉利中牡荆素和异牡荆素[J].理化检验:化学分册,2013,49(2):129-133.WANG Li-ping,LIU Xiao-qin,FAN Hua-jun,et al.HPLC determination of vitexin and isovitexin from Crotalaria sessiliflora L.with response surface method to the optimization of conditions of microwave assisted sample extraction[J].Physical Testing and Chemical Analysis Part B:Chemical Analysis,2013,49(2):129-133.
[25] 黄晓斌,汪欢,陈志鹏,等.Box-Behnken响应面法优化苦参黄酮超声提取工艺[J].中药材,2019(10):2 353-2 357.HUANG Xiao-bin,WANG Huan,CHEN Zhi-peng,et al.Optimization of ultrasonic extraction process of flavonoids from Sophora flavescens by Box-Behnken response surface method[J].Journal of Chinese Medicinal Materials,2019(10):2 353-2 357.
[26] 杨婷婷,房雷雷,辛慧洁,等.响应面法优化超声辅助提取酸枣仁中阿魏酰斯皮诺素[J].食品工业,2019,40(4):151-155.YANG Ting-ting,FANG Lei-lei,XIN Hui-jie,et al.Optimization of ultrasonic assisted extraction 6ʼ-feruloylspinosin from ziziphi spinosae semen by response surface methodology[J].The Food Industry,2019,40(4):151-155.
[27] 李燕,郭兴峰,张华,等.响应面法优化金线草黄酮提取工艺的研究[J].食品研究与开发,2019,40(19):107-113.LI Yan,GUO Xing-feng,ZHANG Hua,et al.Optimization of extraction process of flavonoids from Rubai menbranacea using response surface analysis[J].Food Research and Development,2019,40(19):107-113.
[28] 王晓慧,姚茂君,陈怡君,等.超声辅助复合酶法提取莓茶多糖的工艺优化[J].食品与机械,2021,37(11):166-172.WANG Xiao-hui,YAO Mao-jun,CHEN Yi-jun,et al.Research on extraction technology of ampelopsis grossedentata polysaccharide by ultrasound-assisted enzymatic method[J].Food & Machinery,2021,37(11):166-172.
[29] 周旋,许明祥,蔡文卓,等.女贞子总黄酮超声辅助提取工艺及体外抗氧化活性研究[J].食品与机械,2019,35(3):182-188.ZHOU Xuan,XU Ming-xiang,CAI Wen-zhuo,et al.Ultrasonic-assisted extraction and antioxidant activity of total flavonoids feom Ligustrum Ait by response surface methodology[J].Food & Machinery,2019,35(3):182-188.
[30] 林婷婷,房雷雷,张亚新,等.响应面法优化酸枣仁多糖的脱色工艺[J].食品工业科技,2018,39(24):198-202,208.LIN Ting-ting,FANG Lei-lei,ZHANG Ya-xin,et al.Optimization of decoloration process of Zizyphi Spinosae Semen polysaccharide by response surface methodogy[J].Science and Technology of Food Industry,2018,39(24):198-202,208.
[31] 罗磊,张冰洁,朱文学,等.响应面试验优化超声辅助提取金银花叶黄酮工艺及其抗氧化活性[J].食品科学,2016,37(6):13-19.LUO Lei,ZHANG Bing-jie,ZHU Wen-xue,et al.Ultrasonic-assisted extraction and antioxidation of flavonoids from Lonicera japonica Thunb.Leaves:Process optimization by response surface methodology and antioxidant activity evaluation[J].Food Science,2016,37(6):13-19.