High-throughput detection of copper ions in water by polypeptide probe HWEHH

Authors

XU Di-ya, Hunan Key Laboratory of Special Medical Food Processing, Changsha, Hunan 410000 , China
ZHANG Hai-man, Hunan Key Laboratory of Special Medical Food Processing, Changsha, Hunan 410000 , China
ZHANG Xin, College of Food Science and Engineering, Central South University of Forestry and Technology,Changsha, Hunan 410000 , China ；Hunan Provincial Key Laboratory of Food Safety Monitoring andEarly Warning, Changsha, Hunan 410117 , China
ZHANG Ya-dan, Hunan Key Laboratory of Special Medical Food Processing, Changsha, Hunan 410000 , China
WANG Fang, College of Food Science and Engineering, Central South University of Forestry and Technology,Changsha, Hunan 410000 , China ；Hunan Provincial Key Laboratory of Food Safety Monitoring andEarly Warning, Changsha, Hunan 410117 , China
ZHANG Lin, Hunan Key Laboratory of Special Medical Food Processing, Changsha, Hunan 410000 , China

Abstract

Objective: A high-throughput detection method for Cu²⁺ was developed with a well designed water-soluble fluorescent probe(HWEHH) and the microplate reader. Methods: The specificity of the fluorescent probe for Cu²⁺ determination was studied with the selectivity experiment. The linear range and detection limit of the probe for Cu²⁺ determination was detected with titration experiment, and the accuracy of the probe for the detection of Cu²⁺ in water was studied with sample spiked recovery method. Results: The fluorescent probe was able to specifically detect Cu²⁺. When the concentration of peptide probe was 2 μmol/L, the linear range of Cu²⁺ detection was 0~1.5 μmol/L. The linear equation was Y=1 135.085 7X+2 048.187 6, and the detection limit was 0.036 μmol/L. The binding constant of HWEHH-Cu²⁺ complex was 1.3×106 mol/L. The linear range of high-throughput detection method was 0~1.5 μmol/L, and the recovery rate was 93.6%~101.9%. Conclusion: HWEHH peptide fluorescent probe can be used for high-throughput detection of Cu²⁺ in aqueous solution.

Publication Date

10-16-2022

First Page

60

Last Page

65

DOI

10.13652/j.spjx.1003.5788.2022.80189

Recommended Citation

Di-ya, XU; Hai-man, ZHANG; Xin, ZHANG; Ya-dan, ZHANG; Fang, WANG; and Lin, ZHANG (2022) "High-throughput detection of copper ions in water by polypeptide probe HWEHH," Food and Machinery: Vol. 38: Iss. 8, Article 11.
DOI: 10.13652/j.spjx.1003.5788.2022.80189
Available at: https://www.ifoodmm.cn/journal/vol38/iss8/11

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