Screening of PDE-5 inhibitors in health wine by surface-enhanced Raman spectroscopy

Authors

HU Jia-yong, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Foodfor State Market Regulation, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering andTechnology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China
ZHOU Tao-hong, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Foodfor State Market Regulation, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering andTechnology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China
YAO Xiao-fan, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Foodfor State Market Regulation, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering andTechnology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China
YUAN Min-wen, Huangshi Institue for Food and Drug Test, Huangshi, Hubei 435000 , China
LIU Di, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Foodfor State Market Regulation, Wuhan, Hubei 430075 , China ；Hubei Provincial Engineering andTechnology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China

Abstract

Objective: A surface-enhanced Raman spectroscopy (SERS) method was developed for non-targeted screening of PDE-5 inhibitors in health wine. Methods: An appropriate SERS base of colloidal gold was prepared, and SERS analysis was conducted after the base was mixed with 78 kinds of PDE-5 inhibitors. The spectrogram screening database was established, and whether the matching degree was higher than 70% was used as the basis for judging whether the PDE-5 inhibitors were suspected to be added. Finally, the sensitivity and specificity of the method were investigated. Results: The best method of preparing PDE-5 inhibitors of SERS base was adding 1.0 mL 1% chlorauric acid and 0.7 mL 1% trisodium citrate into 98.3 mL ultrapure water. The lowest concentrations were sildenafil 0.05 mg/L, tadanafil 0.5 mg/L, thiosildenafil 0.05 mg/L and acevardenafil 0.1 mg/L, respectively. The qualitative results of simulated samples were consistent with those of HPLC-MS. Conclusion: In theory, this method can be used to screen non-targeted illegal addition of PDE-5 inhibitors in health wine.

Publication Date

10-16-2022

First Page

64

Last Page

71

DOI

10.13652/j.spjx.1003.5788.2022.90165

Recommended Citation

Jia-yong, HU; Tao-hong, ZHOU; Xiao-fan, YAO; Min-wen, YUAN; and Di, LIU (2022) "Screening of PDE-5 inhibitors in health wine by surface-enhanced Raman spectroscopy," Food and Machinery: Vol. 38: Iss. 9, Article 12.
DOI: 10.13652/j.spjx.1003.5788.2022.90165
Available at: https://www.ifoodmm.cn/journal/vol38/iss9/12

References

[1] 陈玉浩,梁茉刚,蓝康华,等.功能性酒中非法添加西地那非等11种壮阳类药物的调查研究[J].广东化工,2018,45(10):77-79.CHEN Y H,LIANG M G,LAN K H,et al.The study and investigation of eleven kinds of sildenafil drug illegally added in functional wines[J].Guangdong Chemical Industry,2018,45(10):77-79.
[2] MOREIRA S G,BRANNIGAN R E,SPITZ A,et al.Side-effect profile of sildenafil citrate(Viagra)in clinical practice[J].Urology,2000,56(3):474-476.
[3] 雷毅,黄艳婷,陈林,等.高效液相色谱法同时快速测定壮阳类保健品中的18种非法添加物[J].理化检验:化学分册,2016,52(3):249-254.LEI Y,HUANG Y T,CHEN L,et al.Simultaneous rapid determination of 18 illegal additives in impotence healthy products by HPLC[J].Physical Testing and Chemical Analysis Part B:Chemical Analysis.2016,52(3):249-254.
[4] PARK M,AHN S.Quantitative analysis of sildenafil and tadalafil in various fake drugs recently distributed in Korea[J].Journal of Forensic Sciences,2012,57(6):1 637-1 640.
[5] YANG Y J,SONG D M,JIANG W M,et al.Rapid resolution RP-HPLC-DAD method for simultaneous determination of Sildenafil,Vardenafil,and Tadalafil in pharmaceutical preparations and counterfeit drugs[J].Analytical Letters,2010,43(1/2/3):373-380.
[6] 胡婷婷,曲晓宇,康明芹,等.高效液相色谱—高分辨质谱快速筛查和确证保健品中非法添加的壮阳药物[J].色谱,2015(8):897-901.HU T T,QU X Y,KANG M Q,et al.Rapid screening and confirmation of illegally added anti-impotence preparations in health care products by high performance liquid chromatography-high resolution mass spectrometry[J].Chinese Journal of Chromatography,2015(8):897-901.
[7] 张书文,王锟,韩圣谦.超高效液相色谱—串联质谱法同时测定露酒中伐地那非、西地那非和他达那非含量[J].化学分析计量,2016,25(1):44-46.ZHANG S W,WANG K,HAN S Q.Determination of vardenafic-sildenafil and tadalafil in liqueur by UPLC-MS/MS[J].Chemical Analysis and Meterage,2016,25(1):44-46.
[8] 李绍辉,吴寒秋,许秀丽,等.超高效液相色谱串联质谱法检测玛咖产品中非法添加西地那非[J].食品工业科技,2016,37(11):310-313.LI S H,WU H Q,XU X L,et al.Detection of sildenafil illegally added in maca products by liquid chromatography tandem mass spectrometry[J].Science and Technology of Food Industry,2016,37(11):310-313.
[9] ZHANG Y,HUANG Z,DING L,et al.Simultaneous determination of Yohimbine,Sildenafil,Vardenafil and Tadalafil in dietary supplements using high-performance liquid chromatography-tandem mass spectrome-try[J].Journal of Separation Science,2010,33(14):2 109-2 114.
[10] LIN L L,XIU J Z,CHAO Z,et al.Simultaneous determination and determination of Sildenafil and its active metabolite in human plasma using LC-MS/MS method[J].Biomedical Chromatography,2020,34(10):e4927.
[11] LEE J H,MIN A Y,PARK O R,et al.Detection of 94 compounds related to sexual enhancement including Sildenafil,Tadalafil,Vardenafil and their analogues in various formulations of dietary supplements and food samples using HPLC and LC-MS/MS[J].Food Additives & Contaminants:Part A,2021,38(5):769-781.
[12] 庞小雄,吕华冲,张怡红,等.中成药及中药保健品中添加西地那非的快速检测方法[J].中国医院药学杂志,2008,28(8):673-674.PANG X X,LU H C,ZHANG Y H,et al.Rapid determination of Sildenafil in Chinese patent medicines and health products[J].Chinese Journal of Hospital Pharmacy,2008,28(8):673-674.
[13] GUO J B,XU Y,HUANG Z B,et al.Development of an immunoassay for rapid screening of vardenafil and its potential analogues in herbal products based on a group specific monoclonal antibody[J].Analytica Chimica Acta,2009,658(2):197-203.
[14] 彭耀文,申兰慧,杨敏智,等.建立应急检验模型快速筛查及确证片剂中添加的枸橼酸西地那非[J].中国药事,2015,29(3):303-308.PENG Y W,SHEN L H,YANG M Z,et al.Establishment of emergency inspection model for rapid screening and identiifcation of Sildenaifl citrate in tablets[J].Chinese Pharmaceutical Affairs,2015,29(3):303-308.
[15] VREDENBREGT M J,BLOK-TIP L,HOOGERBRUGGE R,et al.Screening suspected counterfeit Viagra and imitations of Viagra with near-infrared spectroscopy[J].Journal of Pharmaceutical & Biomedical Analysis,2006,40(4):840-849.
[16] MANS D J,CALLAHAN R J,DUNN J D,et al.Rapid-screening detection of acetildenafils,sildenafils and avanafil by ion mobility spectrometry[J].Journal of Pharmaceutical and Biomedical Analysis,2013,75:153-157.
[17] 国家市场监督管理总局.食品中那非类药物的测定:BJS 201601[S].北京:国家市场监督管理总局,2016.State Administration for Market Regulation.Determination of non-drugs in food:BJS 201601[S].Beijing:State Administration for Market Regulation,2016.
[18] 国家市场监督管理总局.食品中那非类药物的测定:BJS 201805[S].北京:国家市场监督管理总局,2018.State Administration for Market Regulation.Determination of non-drugs in food:BJS 201805[S].Beijing:State Administration for Market Regulation,2018.
[19] 王红梅,李玲玲,陈海滨,等.表面增强拉曼光谱对西地那非类药物的快速检测[J].高等学校化学学报,2017,38(6):1 040-1 045.WANG H M,LI L L,CHEN H B,et al.Application of Surface-enhanced Raman Spectroscopy in the fast screening of phosphodiesterase type 5 inhibitors[J].Chemical Journal of Chinese Universities,2017,38(6):1 040-1 045.
[20] 吴国萍,周亚红,李静泉,等.表面增强拉曼光谱测定保健品中非法添加物西地那非[J].食品工业科技,2019,40(11):254-259,264.WU G P,ZHOU Y H,LI J Q,et al.Surface Enhanced Raman Spectroscopy(SERS)for the detection of Sildenafil in health care products[J].Science and Technology of Food Industry,2019,40(11):254-259,264.
[21] 胡家勇,张嫚,皮江一,等.表面增强拉曼光谱法定性定量检测保健食品中非法添加物西地那非[J].食品科学,2020,41(8):297-302.HU J Y,ZHANG M,PI J Y,et al.Quantitative and qualitative determination of illicit Sildenafil in health foods by surface enhanced raman spectroscopy[J].Food Science,2020,41(8):297-302.
[22] 吴宝林.金、银纳米粒子的制备及其表面增强拉曼光谱研究[D].武汉:华中农业大学,2010.WU B L.Thepreparation and surface-enhanced Raman Spectroscopy effect of gold and silver nanoparticles[D].Wuhan:Huazhong Agricultural University,2010.
[23] 皋玉婷,陈芮,陈燕.纳米金的合成与表征[J].云南师范大学学报(自然科学版),2016,36(6):46-49.GAO Y T,CHEN R,CHEN Y.Synthesis and characterization of gold Nano-sol[J].Journal of Yunan Normal University(Natural Sciences Edition),2016,36(6):46-49.
[24] ALVAREZ-PUEBLA R A.Effects of the excitation wavelength on the SERS spectrum[J].Journal of Physical Chemistry Letters,2012,3(7):857-866.
[25] 张心敏,史晓凤,韩晓红,等.金溶胶基底的SERS增强效果的实验研究[J].激光生物学报,2014(6):553-559.ZHANG X M,SHI X F,HAN X H,et al.Experimental study on SERS Enhancement of gold colloid[J].Acta Laser Biology Sinica,2014(6):553-559.
[26] 覃玲.食品中非法添加物质筛查模式的研究[D].北京:中国食品药品检定研究院,2018.QIN L.Study on the screening model of illegally added substances in food[D].Beijing:National Institute for Food and Drug Control,2018.