Abstract
Objective: This study aimed to improve the purity of lutein in Marigold. Methods: Lutein was extracted from marigold by sunflower seed oil microemulsion. The crude extract of lutein was saponified with KOH-C2H5OH solution and further purified with macroporous resin. Results: In the static test, HP-20 macroporous resin had the best adsorption and resolution effect of lutein. 80% ethanol solution was used as the adsorption solvent, and anhydrous ethanol solution was used as the analytical solvent, with the loading mass concentration of 0.01 mg/mL. The optimal static adsorption conditions were 30 ℃ temperature, adsorption for 2.0 h, at pH 7.0; the optimal static analytical conditions were temperature 40 ℃, analytical time 1.0 h, at pH 7.0. In the dynamic test, the optimal loading concentration was 0.01 mg/mL, and the loading flow rate was 0.4 mL/min. The purity of lutein after adsorption by macroporous resin reached 61.72%. Conclusion: The purity of lutein in marigold after macroporous resin purification is obviously improved.
Publication Date
10-16-2022
First Page
153
Last Page
158
DOI
10.13652/j.spjx.1003.5788.2022.90190
Recommended Citation
Hong-yan, WU; Yan, SHI; Xiu-xin, LI; and Qi, AN
(2022)
"Optimization of purification process of lutein from Marigold by macroporous resin,"
Food and Machinery: Vol. 38:
Iss.
9, Article 28.
DOI: 10.13652/j.spjx.1003.5788.2022.90190
Available at:
https://www.ifoodmm.cn/journal/vol38/iss9/28
References
[1] 郭耀东,牛仙,任嘉瑜.万寿菊资源综合开发利用研究进展[J].商洛学院学报,2019,33(2):18-23.GUO Y D,NIU X,REN J Y.Research progress on comprehensive development and utilization of marigold resources[J].Journal of Shangluo University,2019,33(2):18-23.
[2] FU X,MA N,SUN W,et al.Microwave and enzyme co-assisted aqueous two-phase extraction of polyphenol and lutein from marigold(Tagetes erecta L.)flower[J].Industrial Crops & Products,2018,123:296-302.
[3] 蒋凌志.玉米蛋白粉中叶黄素的提取工艺研究[D].杭州:浙江大学,2011:33-35.JIANG L Z.Study on extraction technology of lutein from corn gluten powder[D].Hangzhou:Zhejiang University,2011:33-35.
[4] 蔡婧婧.生姜姜黄素纯化、脂质体制备及对小鼠溃疡性结肠炎治疗的研究[D].泰安:山东农业大学,2020:32-38.CAI J J.Study on purification,lipid preparation and treatment of ulcerative colitis in mice[D].Taian:Shandong Agricultural University,2020:32-38.
[5] 许英一,吴红艳,王宇,等.大孔树脂纯化苜蓿叶蛋白肽的工艺优化[J].食品与机械,2019,35(5):167-171.XU Y Y,WU H Y,WANG Y,et al.Optimization of purification process of alfalfa leaf protein peptide by macroporous resin[J].Food & Machinery,2019,35(5):167-171.
[6] 李育楠.玉米叶黄素提取纯化技术研究[D].大庆:黑龙江八一农垦大学,2014:43-48.LI Y N.Study on Extraction and purification technology of lutein from maize[D].Daqing:Heilongjiang Bayi Agricultural Reclamation University,2014:43-48.
[7] KUMAR R,YU W,JIANG C,et al.Improvement of the isolation and purification of lutein from marigold flower(Tagetes erecta L.)and its antioxidant activity[J].Journal of Food Process Engineering,2010,33(6):1 065-1 078.
[8] GIORDANO E,QUADRO L.Lutein,zeaxanthin and mammalian development:Metabolism,functions and implications for health[J].Archives of Biochemistry and Biophysics,2018,647:33-40.
[9] 冯倩,郑伟,关永霞,等.紫花地丁中秦皮乙素、总香豆素大孔树脂纯化工艺研究[J].现代中药研究与实践,2020,34(4):39-43.FENG Q,ZHENG W,GUAN Y X,et al.Study on purification process of Aesculetin and total coumarin in Viola yedoensis by macroporous resin[J].Research and Practice of Modern Traditional Chinese Medicine,2020,34(4):39-43.
[10] ZHANG J,FENG C R,GE P,et al.High purity separation of hypericin from Hypericum perforatum L.extract with macroporous resin column coupling preparative liquid chromatography[J].Process Biochemistry,2021,103:107-113.
[11] 张丽丽.天然叶黄素酯的树脂纯化及叶黄素双乙酰酯的催化合成研究[D].上海:华东理工大学,2011:15-22.ZHANG L L.Study on resin purification of natural lutein ester and catalytic synthesis of luteindiacetyl ester[D].Shanghai:East China University of Technology,2011:15-22.
[12] 张德谨,陈义勇,胡雅琳,等.大孔树脂纯化乌饭树叶黄酮工艺研究[J].食品与机械,2018,34(2):166-170,194.ZHANG D J,CHEN Y Y,HU Y L,et al.Study on purification of flavonoids from black rice leaves by macroporous resin[J].Food & Machinery,2018,34(2):166-170,194.