Optimization and application of Miqu prepared by Rhizopus oryzae from medium-high temperature Daqu

Authors

HUANG Ke-yi, Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application KeyLaboratory of Sichuan Province, Yibin, Sichuan 644000 , China
DENG Jie, Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application KeyLaboratory of Sichuan Province, Yibin, Sichuan 644000 , China ；Liquor Brewing Biotechnology andIntelligent Manufacturing Key Laboratory of China Light Industry, Yibin, Sichuan 644000 , China
WEI Chun-hui, Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application KeyLaboratory of Sichuan Province, Yibin, Sichuan 644000 , China ；Liquor Brewing Biotechnology andIntelligent Manufacturing Key Laboratory of China Light Industry, Yibin, Sichuan 644000 , China
HUANG Zhi-guo, Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application KeyLaboratory of Sichuan Province, Yibin, Sichuan 644000 , China ；Liquor Brewing Biotechnology andIntelligent Manufacturing Key Laboratory of China Light Industry, Yibin, Sichuan 644000 , China
SHI Xuan-chao, Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application KeyLaboratory of Sichuan Province, Yibin, Sichuan 644000 , China ；Liquor Brewing Biotechnology andIntelligent Manufacturing Key Laboratory of China Light Industry, Yibin, Sichuan 644000 , China
REN Zhi-qiang, Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application KeyLaboratory of Sichuan Province, Yibin, Sichuan 644000 , China ；Liquor Brewing Biotechnology andIntelligent Manufacturing Key Laboratory of China Light Industry, Yibin, Sichuan 644000 , China

Abstract

Objective: A microbial enhancer with high-yield glucoamylase was developed from medium-high temperature Daqu and applied to the brewing of Daqu Baijiu for the yield increasing. Methods: Strains with high yield of glucoamylase was isolated from medium-high temperature Daqu and used to make Miqu. Miqu was employed as an enhanced starter in the brewing of Daqu Baijiu, and the yield of Baijiu were tested. Results: A strain M-1 with higher glucoamylase production was isolated and identified as Rhizopus oryzae. The results of the optimized conditions for Miqu making was as follows: bran and rice (2∶8), moisture content 45%, incubation time 72 h, drying temperature 40 ℃. Under the control of these conditions, the glucoamylase activity of Miqu is as high as 864.50 U/g. When appling the Miqu to the brewing of Daqu Baijiu, the results showed that when the addition amount of by, and the yield increased to 43.17% with the addition of 2% (weight of grain) M-1 Miqu. Conclusion: This study established a method for preparing Miqu from Rhizopus oryzae to improve the glucoamylase activity of medium-high temperature Daqu.

Publication Date

10-16-2022

First Page

185

Last Page

190

DOI

10.13652/j.spjx.1003.5788.2022.90178

Recommended Citation

Ke-yi, HUANG; Jie, DENG; Chun-hui, WEI; Zhi-guo, HUANG; Xuan-chao, SHI; and Zhi-qiang, REN (2022) "Optimization and application of Miqu prepared by Rhizopus oryzae from medium-high temperature Daqu," Food and Machinery: Vol. 38: Iss. 9, Article 33.
DOI: 10.13652/j.spjx.1003.5788.2022.90178
Available at: https://www.ifoodmm.cn/journal/vol38/iss9/33

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