Anti-inflammatory activity of Cili extracts and its therapeutic effect on ulcerative colitis model of mice

Authors

WANG Li, State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang, Guizhou 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, Guizhou 550014, China
LI Li-lang, State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang, Guizhou 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, Guizhou 550014, China
LI Qi-ji, State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang, Guizhou 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, Guizhou 550014, China
WANG Yu, State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang, Guizhou 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, Guizhou 550014, China
YANG Xiao-sheng, State Key Laboratory of Functions and Applications of Medicinal Plants, Guiyang, Guizhou 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, Guizhou 550014, ChinaFollow

Corresponding Author(s)

杨小生（1966—），男，贵州省中国科学院天然产物化学重点实验室研究员，博士。E-mail:gzncnp@sina.cn

Abstract

Objective: This study aimed to investigate the effect of Cili extracts in the treatment of enteritis and diarrhea. Methods: The RAW 264.7 cells were treated with different concentrations (1.25, 2.50, 5.00, 10.00, 20.00 μg/mL) of Cili extracts, and then the cell viability was determined by MTT method. RAW264.7 cells were treated with different concentrations of Cili extracts, and then lipopolysaccharide (LPS) was used to induce the cell inflammation model. The content of NO in cell culture supernatant was detected by Griess method. The UC mice model was induced by 3% DSS. The changes of signs during the modeling and drug treatment were observed, and the disease activity index (DAI) was evaluated. The colon length, colon wet weight index and spleen weight were measured. HE staining was used to observe the pathological morphology of colon. The protein expression of Keap1 and Nrf2 in colon tissues were detected by western blot and immunohistochemistry. Results: The concentration of Cili extract ≤10 μg/mL had no significant effect on cell viability (P>0.05). Cili extract inhibited the release of NO, and the inhibitory effect was more obvious with the increase of drug concentration (P<0.05 or P<0.01). Compared with the model group, the colon length of Cili extract group was longer, and the colon wet weight index, spleen weight and DAI fraction were decreased (P<0.05 or P<0.01). HE staining showed that Cili extract had a better protective effect on the intestinal mucosa of UC mice. Western blot and immunohistochemistry showed that Cili extract down-regulated the expression level of Keap1 and up-regulated the expression level of Nrf2. Conclusion: Cili extract had good anti-inflammatory activity in vitro and expressed therapeutic effect on UC mice.

Publication Date

4-25-2023

First Page

164

Last Page

169

DOI

10.13652/j.spjx.1003.5788.2022.90236

Recommended Citation

Li, WANG; Li-lang, LI; Qi-ji, LI; Yu, WANG; and Xiao-sheng, YANG (2023) "Anti-inflammatory activity of Cili extracts and its therapeutic effect on ulcerative colitis model of mice," Food and Machinery: Vol. 39: Iss. 1, Article 27.
DOI: 10.13652/j.spjx.1003.5788.2022.90236
Available at: https://www.ifoodmm.cn/journal/vol39/iss1/27

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