•  
  •  
 

Corresponding Author(s)

孙杨(1988—),男,河南大学副教授,博士。E-mail:sunyangsuofei@qq.com

Abstract

Objective: Building a perfect bioreactor scale-up model provides convenience for enterprise production, and a guidance for reactor manufacturers. Methods: Through the test of 1.5, 5, 20, 200 L scale reactor, the correlation and parameters of the volume dissolved oxygen coefficient kLa and mixing time tm were optimized and explored, the simulation amplification model of bioreactor was established, and the Scaleuper amplification system was built by MATLAB. Results: Compared with the test results, the simulation deviation of kLa was within ±15%. By introducing the definition of critical speed into the correlation of tm, the overall simulation deviation of tm was reduced by 17.4% compared with the original formula. Within ±10%, the simulation deviation of kLa was reduced by embedding BP neural network. The minimum simulation deviation of BP model can reach 0.1% for the training data range. Conclusion: The scale-up model of the reactor based on the empirical correlation has better simulation results after the relevant parameters are measured. It is feasible to use neural network to simulate and amplify the reactor, but it still needs more data training support.

Publication Date

4-25-2023

First Page

18

Last Page

23

DOI

10.13652/j.spjx.1003.5788.2022.80403

References

[1] 刘晓丹. 生物化学制药概述及技术研究进展[J]. 化工设计通讯, 2022, 48(1): 190-193.
[2] 孙杨, 聂简琪, 刘秀霞, 等. 生物过程工程研究在创新生物医药开发中应用的驱动力——生物反应器[J]. 化工进展, 2016, 35(4): 971-980.
[3] 骆海燕, 窦冰然, 姜开维, 等. 搅拌式动物细胞反应器研究应用与发展[J]. 生物加工过程, 2016, 14(2): 75-80.
[4] 高贤申. 生物反应器在设计、制造和使用过程中的质量控制及验证策略探讨[J]. 化工与医药工程, 2020, 41(1): 15-23.
[5] 吴明一, 王红星, 秦玉波, 等. CFD并行计算技术的研究进展[J]. 化工进展, 2007, 26(z1): 15-19.
[6] 银建中, 程绍杰, 贾凌云, 等. 生物反应器放大因素与方法研究[J]. 化工装备技术, 2009, 30(1): 22-27, 8.
[7] 贾士儒. 生物反应工程原理[M]. 北京: 科学出版社, 2015: 308.
[8] LONE S R, KUMAR V, SEAY J R, et al. Mass transfer and rheological characteristics in a stirred tank bioreactor for cultivation of escherichia coli BL21[J]. Biotechnol Bioproc E, 2020, 25: 766-776.
[9] 张庆华, 毛在砂, 杨超, 等. 搅拌反应器中液相混合时间研究进展[J]. 化工进展, 2008, 27(10): 1 544-1 550.
[10] TIKHOMIROVA T S, TARASKEVICH M S, PONOMARENKO O V. The role of laboratory-scale bioreactors at the semi-continuous and continuous microbiological and biotechnological processes[J]. Appl Microbiol Biotechnol, 2018, 102: 7 293-7 308.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.