Determination of bisphenol A and nonylphenol in food packaging material by high performance liquid chromatography-tandem mass spectrometry

Authors

TANG Ji-wang, The Product and Commodity Quality Supervison and Inspection Institute of Hunan Province, Changsha, Hunan 410007, ChinaFollow
YUAN Lie-jiang, The Product and Commodity Quality Supervison and Inspection Institute of Hunan Province, Changsha, Hunan 410007, China
XIAO Yong, The Product and Commodity Quality Supervison and Inspection Institute of Hunan Province, Changsha, Hunan 410007, China
WANG Shu-xia, The Product and Commodity Quality Supervison and Inspection Institute of Hunan Province, Changsha, Hunan 410007, China
PAN Zhao, The Product and Commodity Quality Supervison and Inspection Institute of Hunan Province, Changsha, Hunan 410007, China
DENG Hang, The Product and Commodity Quality Supervison and Inspection Institute of Hunan Province, Changsha, Hunan 410007, China

Abstract

Objective： A method based on high-performance liquid chromatography-mass spectrometry was developed for the simultaneous determination of bisphenol A and nonylphenol in food packaging material. Methods: The extraction solvent and extraction method were investigated. Solid-phase extraction （SPE） conditions were optimized. The effects of solid phase extraction column, loading solvent, eluting solvent and elution solvent on the purification effect were systematically analyzed. The samples were ultrasonic extracted with dichloromethaneand cleaned up on a solid-phase extraction （SPE） cartridge equipped with packing material of silicone. The separation was performed on a Venusil MP C₁₈（2）（100 mm×2.1 mm,2.6 μm）by gradient elution with 0.1% ammonia solution and methanol as mobile phase. The analytes were determined with multiple reaction monitoring （MRM） mode under electrospray negative ionization mode and quantified by an internal standard method. Results: The results indicated that good linearities was obtained for bisphenol A and nonylphenol both within the concentration range of 1.0~200.0 μg/L. The average recoveries of each analyte at three spiked levels of 1.0, 10.0, 200.0 μg/kg were in the range of 89.2%~101.2%, with relative standard deviations （RSD） of 3.1%~6.2%. The limits of detection （LODs） and limits of quantification （LOQs） were 0.5 μg/kg and 1.0 μg/kg, respectively. Conclusion: The method was proved to be a simple, accurate and sensitive method and was successfully applied to detect bisphenol A and nonylphenol in food packaging material. It can also provide reference for the determination of bisphenol A and nonylphenol in food.

Publication Date

4-25-2023

First Page

37

Last Page

41

DOI

10.13652/j.spjx.1003.5788.2022.80371

Recommended Citation

Ji-wang, TANG; Lie-jiang, YUAN; Yong, XIAO; Shu-xia, WANG; Zhao, PAN; and Hang, DENG (2023) "Determination of bisphenol A and nonylphenol in food packaging material by high performance liquid chromatography-tandem mass spectrometry," Food and Machinery: Vol. 39: Iss. 1, Article 6.
DOI: 10.13652/j.spjx.1003.5788.2022.80371
Available at: https://www.ifoodmm.cn/journal/vol39/iss1/6

References

[1] WANG L, GUO M, FENG G L, et al. Effects of chronic exposure to nonylphenol at environmental concentration on thyroid function and thyroid hyperplasia disease in male rats[J]. Toxicology, 2021, 461: 152918.
[2] LIU C, NI C, LIU W, et al. Effects of long-term nonylphenol exposure on myocardial fibrosis and cardiac function in rats[J]. Environmental Sciences Europe, 2021, 33（1）: 1-6.
[3] 谢明勇, 刘晓珍, 陈泱杰. 壬基酚在食品中的污染现状及其生物毒性概述[J]. 食品科学技术学报, 2014, 32（1）: 1-7.
[4] YANG Q, XU W H, LUAN T G, et al. Comparative responses of cell growth and related extracellular polymeric substances in Tetraselmis sp. to nonylphenol, bisphenol A and 17 α-ethinylestradiol[J]. Environmental Pollution, 2021, 274: 116605.
[5] JIA X X, YAO Z Y, LIU S, et al. Suspension array for multiplex immunoassay of five common endocrine disrupter chemicals[J]. Microchimica Acta, 2021, 188（9）: 1-9.
[6] ZENG L S, ZHANG X, WANG X, et al. Simultaneous fluorescence determination of bisphenol A and its halogenated analogs based on a molecularly imprinted paper-based analytical device and a segment detection strategy[J]. Biosensors & Bioelectronics, 2021, 16: 113106.
[7] ZHANG L, CHEN Y S, ZHU Q, et al. SERS based immunochromatographic assay for rapid and quantitative determination of bisphenol A[J]. Vibrational Spectroscopy, 2021, 113: 103225.
[8] 陈旭明, 李婷. 塑料食品接触材料中壬基酚含量的测定[J]. 包装与食品机械, 2018, 36（2）: 68-70, 47.
[9] MAHDAVIANPOUR M, CHAMKOURI N, CHAMKOURI H, et al. Determination of bisphenol a migration from food packaging by dispersive liquid-liquid microextraction[J]. MethodsX, 2021, 8: 101415.
[10] 马强, 白桦, 王超, 等. 高效液相色谱法对纺织品和食品包装材料中的壬基酚、辛基酚和双酚A的同时测定[J]. 分析测试学报, 2009, 28（10）: 1 160-1 164.
[11] 刘苗, 朱惠扬, 甘平胜, 等. 气相色谱—三重四级杆串联质谱法检测广州市饮用水中3种酚类污染物含量[J]. 中国卫生检验杂志, 2017, 27（2）: 163-165, 173.
[12] FERNNDEZ M F, MUSTIELES V, SUREZ B, et al. Determination of bisphenols, parabens, and benzophenones in placenta by dispersive liquid-liquid microextraction and gas chromatography-tandem mass spectrometry[J]. Chemosphere, 2021, 274: 129707.
[13] 毛丽莎, 姜杰, 陈慧玲, 等. 固相萃取—液相色谱/串联质谱法测定尿中10种双酚类物质和壬基酚及辛基酚化合物[J]. 预防医学情报杂志, 2020, 36（2）: 212-218.
[14] 黎绍学, 杨俊, 曾莹, 等. LC-MS/MS测定食品接触材料中烷基酚类物质的溶出暴露水平[J]. 食品工业, 2018, 39（2）: 320-324.
[15] AO J J, ZHANG Q L, TANG W F, et al. A simple, rapid and sensitive method for the simultaneous determination of eighteen environmental phenols in human urine[J]. Chemosphere, 2021, 278: 130494.