Determination of abietic acid compounds in poultry by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry coupled with dispersive solid phase extraction

Authors

LIN Xiaojun, Guangzhou Inspection and Testing Certification Group Co., Ltd., National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, ChinaFollow
YAN Wanying, Guangzhou Inspection and Testing Certification Group Co., Ltd., National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
HE Minheng, Guangzhou Inspection and Testing Certification Group Co., Ltd., National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
HUANG Jiale, Guangzhou Inspection and Testing Certification Group Co., Ltd., National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
WU Yiqi, Guangzhou Inspection and Testing Certification Group Co., Ltd., National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
YE Ling, Guangzhou Inspection and Testing Certification Group Co., Ltd., National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
ZHANG Ruirui, Guangzhou Inspection and Testing Certification Group Co., Ltd., National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China

Abstract

Objective： An ultra performance liquid chromatography-quadrupole/time-of-flight mass spectrometry （UPLC-Q-TOF/MS） method was established for the determination of abietic acids in poultry. Methods： The samples were extracted with acetonitrile, then salted out and stratified. The supernatant was purified by C₁₈ solid phase extractant and finally determined by UPLC-Q-TOF/MS in ESI negative mode. Results： Abietic acid compounds exhibited a good linear relationship in the concentration range of 10~500 μg/L with good correlation coefficient R²>0.998. The average recoveries of three abietic acid compounds were experiments, the average recovery rate was 87.8%~98.5% with relative standard deviations （RSDs, n=6） of 1.99%~6.71%, at the spiked level of 20, 40, 200 μg/kg. The limits of detection （LODs） were 10 μg/kg and the method limits of quantification （LOQs） were 20 μg/kg. Conclusion： This method was convenient operation, rapid, high recovery and sensitivity and good precision that could meet the determination requirements of three abietic acids in poultry.

Publication Date

12-26-2023

First Page

75

Last Page

79

DOI

10.13652/j.spjx.1003.5788.2022.81221

Recommended Citation

Xiaojun, LIN; Wanying, YAN; Minheng, HE; Jiale, HUANG; Yiqi, WU; Ling, YE; and Ruirui, ZHANG (2023) "Determination of abietic acid compounds in poultry by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry coupled with dispersive solid phase extraction," Food and Machinery: Vol. 39: Iss. 10, Article 11.
DOI: 10.13652/j.spjx.1003.5788.2022.81221
Available at: https://www.ifoodmm.cn/journal/vol39/iss10/11

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