Determination of lactose in milk by high performance liquid chromatography-evaporative light scattering detector with RCM-monosaccharide Ca²⁺ column

Authors

ZHU Xiaopeng, College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an, Shaanxi 710062, China
FU Shangchen, College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an, Shaanxi 710062, China
HAO Guo, Fuping County Inspection and Testing Center, Weinan, Shaanxi 711700, China
NIU Pengfei, College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an, Shaanxi 710062, China
LIU Yongfeng, College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an, Shaanxi 710062, ChinaFollow

Corresponding Author(s)

刘永峰（1981—），男，陕西师范大学教授，博士。E-mail: yongfeng200@126.com

Abstract

Objective: To achieve rapid detection of lactose content in milk, a new method for the determination of lactose in milk based on RCM-monosaccharide Ca²⁺ column and high-performance liquid chromatography has been established. Methods: Milk was precipitated with protein in a 0.2 g/mL trichloroacetic acid solution, and the resulting filtrate was diluted 100 times and filtered with a membrane before entering a high-performance liquid chromatography system. It was separated by an RCM-monosaccharide Ca²⁺ column and detected by an evaporative light scattering detector. Results: The results showed that lactose had a good correlation between peak area and concentration in the linear range （20～100 mg/L）, with an R² of 0.999 8. When the lactose spiking levels were 15, 40, and 80 mg/g, the recovery range was between 90.96% and 98.23%, the detection limit was 3.6 μg/g, and the quantitative limit was 12 μg/g, the lactose concentration was determined within 6 minutes. 11 commercial milk samples were measured by this method, and the results were consistent with the national standard method. Conclusion: The precision, repeatability, and recovery rate of this method met relevant regulations, and the detection results were accurate and time-consuming. It is suitable for rapid detection of lactose content in milk.

Publication Date

12-26-2023

First Page

58

Last Page

63

DOI

10.13652/j.spjx.1003.5788.2023.80388

Recommended Citation

Xiaopeng, ZHU; Shangchen, FU; Guo, HAO; Pengfei, NIU; and Yongfeng, LIU (2023) "Determination of lactose in milk by high performance liquid chromatography-evaporative light scattering detector with RCM-monosaccharide Ca²⁺ column," Food and Machinery: Vol. 39: Iss. 11, Article 10.
DOI: 10.13652/j.spjx.1003.5788.2023.80388
Available at: https://www.ifoodmm.cn/journal/vol39/iss11/10

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