Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee

Authors

GAO Yunkai, Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100, China
CHEN Xiaomei, Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100, China
CHEN Chunqian, Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100, China
ZHOU Lingyu, Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100, China
DENG Yinglin, Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100, China
YIN Qingchun, Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou, Hainan 570100, ChinaFollow

Corresponding Author(s)

尹青春（1986—），女，海南省食品检验检测中心高级工程师，硕士。E-mail: yinqingchun@163.com

Abstract

Objective： A new method was developed for the simultaneous determination of three kinds of ochratoxins in coffee by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with QuEChERS pretreatment. Methods： Samples were extracted by acetonitri-water-formic acid solution (55∶40∶5) with ultrasonic, salted out with a QuEChERS salt pocket, and then purified with a (ZanChERS-Myco17) column. The chromatographic separation was performed on an ACQUITY UPLC BEH C₁₈ (1.7 μm, 2.1 mm×100 mm) column by gradient elution using a mobile phase comprising 0.2% formic acid aqueous solution and acetonitrile. In the positive ion mode of electric spray, the samples detected were used by multiple reaction monitoring (MRM) modes with internal standard method. Results： Three target substances had a good linear relationship in the range of 0.1~20.0 ng/mL, and the correlation coefficient was greater than 0.999 46. The detection limit and quantitative limit were 0.1~0.2 μg/kg and 0.2~0.7 μg/kg, respectively. Average recoveries at three spiked levels in the coffee matrix were 79.0%~103.3% with a relative standard deviation of 1.5%~7.6%. Conclusion： This method has the advantages of simple sample pretreatment, good reproducibility and high efficiency, and is suitable for the high-throughput detection of multiple ochratoxins in different coffee samples.

Publication Date

12-26-2023

First Page

91

Last Page

97

DOI

10.13652/j.spjx.1003.5788.2023.80084

Recommended Citation

Yunkai, GAO; Xiaomei, CHEN; Chunqian, CHEN; Lingyu, ZHOU; Yinglin, DENG; and Qingchun, YIN (2023) "Establishment of QuEChERS-UPLC-MS/MS method to determine three kinds of ochratoxins in coffee," Food and Machinery: Vol. 39: Iss. 11, Article 15.
DOI: 10.13652/j.spjx.1003.5788.2023.80084
Available at: https://www.ifoodmm.cn/journal/vol39/iss11/15

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