Abstract
Objective: This study aimed to realize the high-value utilization of biological active ingredients and anti-inflammatory peptides from the Pinctada martensii meat. Methods: The hydrolysis process of the P. martensii meat was optimized by single factor test and response surface test with degree of hydrolysis (DH) as the index. The amino acid composition and anti-inflammatory activity of P. martensii meat hydrolysate peptides were evaluated and analyzed. Results: The results showed that neutral protease was the optimal enzyme, and the optimal hydrolysis conditions were solid-liquid ratio 1∶1 (g/mL), temperature 46.3 ℃, hydrolysis time 1.4 h, and substrate ratio 0.3%. The hydrolysis degree was 22.88%, which was not significantly different from the theoretical value, and the regression model was reliable. The hydrolysates including 19.84% of essential amino acids, 21.19% of hydrophobic amino acids and 10.46% of positively charged amino acids. In LPS induced RAW264.7 anti-inflammatory model of mouse macrophages, the hydrolyzed peptide of P. martensii had no cytotoxicity and was beneficial to the proliferation of macrophages in the mass concentration range of 0~4.0 mg/mL. At the mass concentration of 2.0 mg/mL, hydrolyzed peptide could effectively inhibit the production of NO and inflammatory cytokines TNF-α, IL-6 and IL-1β; the inhibition rate of NO, TNF-α, IL-6 and IL-1β was 70.00%, 83.01%, 85.04% and 83.11%, respectively. Conclusion: Hydrolytic peptide of P. martensii has a complete range of amino acids, which can effectively inhibit the production of NO and inflammatory cytokines TNF-α, IL-6 and IL-1β in RAW264.7 macrophages, showing good anti-inflammatory activity.
Publication Date
4-25-2023
First Page
132
Last Page
139,206
DOI
10.13652/j.spjx.1003.5788.2022.80425
Recommended Citation
Jin-peng, SHEN; Ke-wen, WANG; Pan-tian, HUANG; Bing-bing, CHEN; Zhen, XIA; Ying-kun, LI; Xiang-hua, WANG; Yong, CAO; Jian-yin, MIAO; and Bi-min, LIN
(2023)
"Preparation, amino acid composition and anti-inflammatory activity of hydrolyzed peptides from Pinctada martensii,"
Food and Machinery: Vol. 39:
Iss.
2, Article 22.
DOI: 10.13652/j.spjx.1003.5788.2022.80425
Available at:
https://www.ifoodmm.cn/journal/vol39/iss2/22
References
[1] ABD E R, MAHGOUB S, EL-SHANBAKY H M, et al. Utility of novel 2-furanones in synthesis of other heterocyclic compounds having anti-inflammatory activity with dual COX2/LOX inhibition[J]. Journal of Enzyme Inhibition and Medicinal Chemistry, 2021, 36（1）: 977-986.
[2] GAO R, SHU W, SHEN Y, et al. Sturgeon protein-derived peptides exert anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via the MAPK pathway[J]. Journal of Functional Foods, 2020, 72: 104044.
[3] FENG M, WANG X, XIONG H, et al. Anti-inflammatory effects of three selenium-enriched brown rice protein hydrolysates in LPS-induced RAW264.7 macrophages via NF-κB/MAPKs signaling pathways[J]. Journal of Functional Foods, 2021, 76: 104320.
[4] MANKHONG S, IAWSIPO P, SRISOOK E, et al. 4-methoxycinnamyl p-coumarate isolated from Etlingera pavieana rhizomes inhibits inflammatory response via suppression of NF-κB, Akt and AP-1 signaling in LPS-stimulated RAW 264.7 macrophages[J]. Phytomedicine, 2019, 54: 89-97.
[5] GUPTA S, SHARMA A K, SHASTRI V, et al. Prediction of anti-inflammatory proteins/peptides: An insilico approach[J]. Journal of Translational Medicine, 2017, 15（1）: 1-11.
[6] GILROY D, DE M R. New insights into the resolution of inflammation[J]. Seminars in Immunology, 2015, 27（3）: 161-168.
[7] GONZALEZ-REY E, ANDERSON P, DELGADO M. Emerging roles of vasoactive intestinal peptide:A new approach for autoimmune therapy[J]. Annals of the Rheumatic Diseases, 2007, 66（suppl 3）: iii70-iii76.
[8] DELGADO M, GANEA D. Anti-inflammatory neuropeptides: A new class of endogenous immunoregulatory agents[J]. Brain, Behavior, and Immunity, 2008, 22（8）: 1 146-1 151.
[9] GAO R, SHU W, SHEN Y, et al. Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways[J]. Food Science and Human Wellness, 2021, 10（1）: 103-111.
[10] QIAN B, ZHAO X, YANG Y, et al. Antioxidant and anti-inflammatory peptide fraction from oyster soft tissue by enzymatic hydrolysis[J]. Food Science & Nutrition, 2020, 8（7）: 3 947-3 956.
[11] YU W, FIELD C J, WU J. Purification and identification of anti-inflammatory peptides from spent hen muscle proteins hydrolysate[J]. Food Chemistry, 2018, 253: 101-107.
[12] 章超桦, 吴红棉, 洪鹏志, 等. 马氏珠母贝肉的营养成分及其游离氨基酸组成[J]. 水产学报, 2000（2）: 180-184.
[13] 杨发明, 林海生, 秦小明, 等. 珍珠贝外套膜酶解产物促进小鼠皮肤创伤愈合作用研究[J]. 大连海洋大学学报, 2019, 34（4）: 492-498.
[14] 刘晓月, 黄丽, 徐羽, 等. 马氏珠母贝综合利用的研究进展[J]. 食品研究与开发, 2020, 41（2）: 200-204.
[15] ADDLER-NISSEN J. Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzene sulfonic acid[J]. Journal of Agricultural and Food Chemistry, 1979, 27（6）: 1 256-1 262.
[16] PANASIUK R, AMAROWICZ R, KOSTYRA H, et al. Determination of α-amino nitrogen in pea protein hydrolysates: A comparison of three analytical methods[J]. Food Chemistry, 1998, 62（3）: 363-367.
[17] 焦宇知. 酪蛋白磷酸肽新生产工艺及其功能性质研究[D]. 无锡: 江南大学, 2004: 63.
[18] KLOMPONG V, BENJAKUL S, KANTACHOTE D, et al.Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally （Selaroides leptolepis） as influenced by the degree of hydrolysis and enzyme type[J]. Food Chemistry, 2007, 102（4）: 1 317-1 327.
[19] 马骏, 石晓丹, 王家林. 酶解法制备啤酒糟抗氧化肽工艺优化[J]. 食品科技, 2021, 46（9）: 73-78.
[20] 尹乐斌, 刘丹, 廖聪, 等. 酶解豆清液制备抗氧化肽[J]. 食品工业, 2021, 42（5）: 177-181.
[21] 沈唯军, 时晨, 丁婷. 应用固定化α-淀粉酶探究温度对酶活性的影响[J]. 生物学教学, 2018, 43（3）: 44-45.
[22] 范三红, 胡雅喃, 何亚. 响应面法优化菊芋渣酶解制备抗氧化肽工艺[J]. 食品科学, 2015, 36（8）: 49-53.
[23] 刘东伟, 袁玮琼, 柳梅, 等. 核桃粕蛋白抑菌肽的制备工艺及纯化[J]. 食品工业科技, 2021, 42（2）: 185-191.
[24] 张维, 胡馨月, 赵行, 等. 响应面法优化紫贻贝鲜味肽酶法制备工艺[J]. 食品工业科技, 2021, 42（8）: 206-214.
[25] 王安凤, 赵永强, 陈胜军, 等. 响应面法优化合浦珠母贝肉水解工艺[J]. 食品与发酵工业, 2017, 43（11）: 165-171.
[26] GUHA S, MAJUMDER K. Structural-features of food-derived bioactive peptides with anti-inflammatory activity: A brief review[J]. Journal of Food Biochemistry, 2019, 43（1）: e12531.
[27] CHEN X, CHEN Y, DAI L, et al. MiR-96-5p alleviates inflammatory responses by targeting NAMPT and regulating the NF-κB pathway in neonatal sepsis[J]. Bioscience Reports, 2020, 40（7）: BSR20201267.