Application of flow cytometry in microbial detection of dairy products

Authors

QI Yan, State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China; Shanghai Engineering Research Center of Dairy Biotechnology, Shanghai 200436, China; Dairy Research Institute, Bright Dairy and Food Co., Ltd., Shanghai 200436, China
YOU Chun-ping, State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China; Shanghai Engineering Research Center of Dairy Biotechnology, Shanghai 200436, China; Dairy Research Institute, Bright Dairy and Food Co., Ltd., Shanghai 200436, ChinaFollow

Corresponding Author(s)

游春苹（1982—），女，光明乳业股份有限公司正高级工程师，博士。E-mail:youchunping@brightdairy.com

Abstract

The principles and structures of flow cytometer, the methods and applications of flow cytometry in microbial detection of dairy products were introduced in this review, and the future research direction was prospected.

Publication Date

4-25-2023

First Page

221

Last Page

226,235

DOI

10.13652/j.spjx.1003.5788.2022.80699

Recommended Citation

Yan, QI and Chun-ping, YOU (2023) "Application of flow cytometry in microbial detection of dairy products," Food and Machinery: Vol. 39: Iss. 2, Article 35.
DOI: 10.13652/j.spjx.1003.5788.2022.80699
Available at: https://www.ifoodmm.cn/journal/vol39/iss2/35

References

[1] 郭本恒. 乳品安全[M]. 北京: 化学工业出版社, 2015: 3, 179-195.
[2] 陈嘉惠, 陈沁, 钮冰. 乳品中有害微生物检测技术的研究进展[J]. 中国乳品工业, 2019, 47（5）: 32-36.
[3] 韩利慧. 乳制品中快速微生物检测技术分析[J]. 中国标准化, 2018（24）: 185-186.
[4] 刘婷婷, 于靓, 孟冬青, 等. 流式细胞术在液体基质食品检测中的应用研究进展[J]. 上海师范大学学报, 2020, 49（6）: 630-636.
[5] 夏天爽. 流式细胞术在食品微生物检测领域的研究进展[J]. 食品安全导刊, 2019（34）: 62-64.
[6] MCKINNON K M. Flow cytometry: An overview[J]. Current Protocols in Immunology, 2018, 120: 5.1.1-5.1.11.
[7] 杭海英, 刘春春, 任丹丹. 流式细胞术的发展、应用及前景[J]. 中国生物工程杂志, 2019, 39（9）: 68-83.
[8] 赵书涛, 武晓东, 王策, 等. 流式细胞仪的原理、应用及最新进展[J]. 现代生物医学进展, 2011, 11（22）: 4 378-4 381.
[9] CASTILLO-HAIR S. FlowCal: Software for analysis and calibration of flow cytometry data. （2016-07-06）[2022-04-21]. https://benchling.com/pub/tabor-flowcal.
[10] 王建红. 流式细胞技术在医学检验中的应用研究进展[J]. 临床检验杂志, 2017, 6（1）: 149-150.
[11] 张艺. 流式细胞仪构成与工作原理[J]. 医疗设备信息, 2005, 20（8）: 25-26.
[12] 何克建. 流式细胞技术与流式细胞仪[J]. 医疗设备, 2000, 13（5）: 6-8.
[13] WANG B, LIU S, SUI Z, et al. Rapid flow cytometry detection of single viable Salmonella cells in milk powder[J]. Foodborne Pathogens and Disease, 2020, 17（7）: 447-458.
[14] YANACHKINA P, MCCARTHY C, GUINEE T, et al. Effect of varying the salt and fat content in Cheddar cheese on aspects of the performance of a commercial starter culture preparation during ripening[J]. International Journal of Food Microbiology, 2016, 224: 7-15.
[15] 孙芝杨, 黄闯, 李新建, 等. 流式细胞法对乳制品细菌总数的快速检测研究[J]. 食品工业, 2016, 37（11）: 180-182.
[16] 刘思渊, 古少鹏, 隋志伟, 等. 流式分析技术快速定量检测牛乳中大肠杆菌O157:H7[J]. 食品科学, 2018, 39（6）: 302-306.
[17] GUNASEKERA T S, ATTFIELD P V, VEAL D A, et al. A flow cytometry method for rapid detection and enumeration of total bacteria in milk[J]. Applied and Environmental Microbiology, 2000, 66（3）: 1 228-1 232.
[18] SAHALAN A Z, AZIZ A H A, LIAN H H, et al. Divalent cations （Mg²⁺, Ca²⁺） protect bacterial outer membrane damage by polymyxin B[J]. Sains Malaysiana, 2013, 42（3）: 301-306.
[19] KATHRIN T, BERGMILLER T, GUET C. Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli stains[J]. Journals & Biotechnology, 2018, 268: 40-52.
[20] HARUTA S, LINO T, OHKUM A, et al. Ca²⁺ in hybridization solutions for fluorescence in situ hybridization facilitates the detection of Enterobacteriaceae[J]. Microbes and Environments, 2017, 32（2）: 142-146.
[21] 王锐敏, 贾瑞宝, 逯南南, 等. 流式细胞术在水质检测领域的研究进展[J]. 中国环境检测, 2020, 36（3）: 114-121.
[22] 杨莉婷, 何丽, 何海宁, 等. 流式细胞术对生乳中微生物检测的应用研究[J]. 广西师范大学学报, 2017, 35（2）: 112-116.
[23] BUNTHOF C J, ABEE T. Development of a flow cytometric method to analyze subpopulations of bacteria in probiotic products and dairy starters[J]. Applied and Environmental Microbiology, 2002, 68（6）: 2 934-2 942.
[24] CASSOLI L D, LIMA W J, ESGUERRA J C, et al. Do different standard plate counting （IDF/ISSO or AOAC） methods interfere in the conversion of individual bacteria counts to colony forming units in raw milk[J]. Journal of Applied Microbiology, 2016, 121（4）: 1 052-1 058.
[25] HE S, HONG X, HUANG T, et al. Rapid quantification of live/dead lactic acid bacteria in probiotic products using high-sensitivity flowcytometry[J]. Methods and Applications in Fluorescence, 2017, 5（2）: 024002.
[26] GENNOVESE M, POULAIN E, DOPPLER F, et al. Bacillus spore enumeration using flow cytometry: A proof of concept for probiotic application[J]. Journal of Microbiological Methods, 2021, 190: 106336.
[27] POUDEL R, THUNELL R K, OBERG C J, et al. Comparison of growth and survival of single strains of Lactococcus lactis and Lactococcus cremoris during cheddar cheese manufacture[J]. Journal of Dairy Science, 2022, 105（3）: 2 069-2 081.
[28] BENSCH G, RGER M, WASSERMANN M, et al. Flow cytometric viability assessment of lactic acid bacteria starter cultures produced by fluidized bed drying[J]. Applied Microbiology and Biotechnology, 2014, 98（11）: 4 897-4 909.
[29] WNUK M, LEWINSKA A. Imaging flow cytometry-based analysis of bacterial profiles in milk samples[J]. Food and Bioproducts Processing, 2021, 128: 102-108.
[30] GENG J, CHIRON C, COMBRISSON J. Rapid and specific enumeration of viable Bifidobacteria in dairy products based on flow cytometry technology: A proof of concept study[J]. International Dairy Journal, 2014, 37: 1-4.
[31] GANDHI A, SHAH N P. Effect of salt on cell viability and membrane integrity of Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium longum as observed by flow cytometry[J]. Food Microbiology, 2015, 49: 197-202.
[32] LIU S, WANG B, SUI Z, et al. Faster detection of Staphylococcus aureus in milk and milk powder by flow cytometry[J]. Foodborne Pathogen and Disease, 2021, 18（5）: 346-353.
[33] 梅仕良, 姜凯, 张娜娜, 等. 流式细胞仪检测技术在食源性致病菌检测中的应用[J]. 上海师范大学学报（自然科学版）, 2017, 46（5）: 757-761.
[34] 李萍, 温平威, 许恒毅, 等. 流式细胞术在食源致病菌检测中的应用研究进展[J]. 食品工业科技, 2013, 34（14）: 375-379.
[35] 王亚利, 包秋华, 王俊国, 等. 乳酸菌活的非培养态的研究进展[J]. 中国乳品工业, 2016, 44（3）: 41-45.
[36] 郭慧玲, 邵玉宇, 高姝冉, 等. 细菌活的非可培养态研究[J]. 中国乳品工业, 2014, 42（1）: 31-36.
[37] 李静薇, 杨雅迪, 李婷婷, 等. VBNC细菌的抗生素耐受机制及治疗研究进展[J]. 国外医药（抗生素分册）, 2022, 43（1）: 1-9.
[38] 赵黎黎, 包秋华, 赵国芬. 细菌VBNC态检测技术的研究进展[J]. 微生物学杂志, 2016, 36（4）: 96-101.
[39] ZHANG J, WANG L, SHI L, et al. Survival strategy of Cronobacter sakazakii against ampicillin pressure: Induction of the viable but nonculturable state[J]. International Journal of Food Microbiology, 2020, 334: 108819.
[40] LI N, SANTILLAN-URQUIZA E, CRONIN U, et al. Assessment of the response of indigenous microflora and inoculated Bacillus licheniformis endospores in reconstituted skim milk to microwave and conventional heating systems by flow cytometry[J]. Journal of Dairy Science, 2021, 104（9）: 9 627-9 644.
[41] MIESZKIN S, HYMERY N, DEBAETS S, et al. Action mechanisms involved in the bioprotective effect of Lactobacillus harbinensis K.V9.3.1.Np against Yarrowia lipolytica in fermented milk[J]. International Journal of Food Microbiology, 2017, 248: 47-55.
[42] 杜耿记, 金珠, 吴小慧, 等. 流式细胞仪快速检测酸奶中乳酸菌含量[J]. 食品安全质量检测学报, 2017, 8（7）: 2 810-2 814.
[43] 王杰, 郑亦舟, 姜凯, 等. 乳酸菌计数结果的比较研究[J]. 上海师范大学学报（自然科学版）, 2018, 47（6）: 713-718.
[44] CHIRON C, TOMPKINS T A, BURGUIRE P. Flow cytometry: A versatile technology for specific quantification and viability assessment of microorganisms in muti-strain probiotic products[J]. Journal of Applied Microbiology, 2018, 124（2）: 572-584.
[45] BARROS C, FULAZ S, VITALE S, et al. Interactions between functionalised silica nanoparticles and Pseudomonas fluorescens biofilm matrix: A focus on the protein corona[J]. PLoS One, 2020, 15（7）: e0236441.
[46] 张云, 邓彬. 幽门螺杆菌生物膜的形成及其在抗生素耐药中的作用[J]. 国际医药卫生导报, 2020, 26（22）: 3 375-3 378.