Determination of altrenogest residue in pig edible tissue by a high performance liquid chromatography-tandem mass spectrometry method

Authors

NI Xiang-yan, Beijing Agricultural Product Quality and Safety Center, Beijing 102629, China
SUN Nian-xia, Anhui University of Chinese Medicine, Hefei, Anhui 230012, China
YU Han-bing, Beijing Agricultural Product Quality and Safety Center, Beijing 102629, China
FANG Fang, Beijing Agricultural Product Quality and Safety Center, Beijing 102629, China
SUN Zhi-wen, Beijing Agricultural Product Quality and Safety Center, Beijing 102629, China

Corresponding Author(s)

孙志文（1976—），男，北京市农产品质量安全中心高级兽医师，硕士。E-mai: Sunzw673@163.com

Abstract

Objective: To determinate the residues of altrenogest in porcine edible tissues （muscle, liver, fat）, a high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） method was established. Methods: The samples were hydrolyzed with β-glucuronidase/arylsulfase, extracted by acetonitrile （ACN）, purified by PRiME HLB solid phase extraction column. LC separation was performed with Waters UPLC BEH C₁₈ reversed-phase chromatography with a linear gradient elution of acetonitrile （ACN）-0.1% formic acid （FA） as the mobile phase. The samples were detected by electrospray in positive ion mode. Results: The pregnancin in tissues were quantified in the range of 1～50 μg/L with good linearity and high linear correlation coefficient （R²>0.99）. The limit of detection （LOD, S/N=3） and the limit of quantification （LOQ, S/N=10） were as low as 0.5 μg/kg and 1.0 μg/kg separately. Under three different concentrations （1.0, 2.0, 10.0 μg/kg）, the average recoveries were 60.9%～89.8%, the intra-day coefficient of variation was 0.8%～7.1%, and the inter-day coefficient of variation was 2.5%～11.6%. Conclusion: The method is simple and suitable for the qualitative and quantitative analysis of altrenogest residue in porcine edible tissues.

Publication Date

4-25-2023

First Page

45

Last Page

49

DOI

10.13652/j.spjx.1003.5788.2022.60082

Recommended Citation

Xiang-yan, NI; Nian-xia, SUN; Han-bing, YU; Fang, FANG; and Zhi-wen, SUN (2023) "Determination of altrenogest residue in pig edible tissue by a high performance liquid chromatography-tandem mass spectrometry method," Food and Machinery: Vol. 39: Iss. 2, Article 7.
DOI: 10.13652/j.spjx.1003.5788.2022.60082
Available at: https://www.ifoodmm.cn/journal/vol39/iss2/7

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