Evaluation of inhibitory activity of a polyoxometallate on polyphenol oxidase and its application in the preservation of Litopenaeus vannamei

Authors

FANG Jia-qi, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China
HAN Qing, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China
MA Yang-yu, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China
WANG Fang, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, ChinaFollow
LI Li-li, College of Ocean Food and Biologicol Engineering, Jimei University, Xiamen, Fujian 361021, China
LI Wen-jie, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; College of Chemical Engineering and Materials, Quanzhou Normal University, Quanzhou, Fujian 362000, China
WANG Li, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; College of Ocean Food and Biologicol Engineering, Jimei University, Xiamen, Fujian 361021, China

Corresponding Author(s)

王芳（1969—）, 女, 泉州师范学院教授, 硕士生导师, 博士。 E-mail: dwf320@163.com

Abstract

Objective: To design new and effective polyphenol oxidase （PPO） inhibitors and expand their application in the field of aquatic product preservation. Methods: The synthesized H₈[P₂Mo₁₇Ni（OH₂）O₆₁] （P₂Mo₁₇Ni） structure was characterized by ultraviolet and infrared spectrum. The inhibitory effect, type and mechanism of P₂Mo₁₇Ni on the relative activity of mushroom PPO in vitro was clarified. Then, the preservation effects of P₂Mo₁₇Ni on Litopenaeus vannamei Boone were evaluated through studying the index change of shrimps treated with P₂Mo₁₇Ni stored at 4 ℃, in which included the sensory score, the value of chromatic aberration L*, the value of total chromatic aberration （ΔE）, the total number of colonies, the value of pH and the value of total volatile basic nitrogen （TVB-N）. Results: The results showed that P₂Mo₁₇Ni was synthesized successfully. P₂Mo₁₇Ni inhibited the activity of PPO from mushroom in vitro with semi-inhibitory concentration （IC₅₀） of （0.377±0.004） mmol/L. P₂Mo₁₇Ni inhibited mushroom PPO in a competitive reversible manner, and the inhibition constant K_I was 0.185 mmol/L. Under the cold storage condition of 4 ℃, compared with the control group, P₂Mo₁₇Ni could effectively delay the decline of sensory score and the increase of L* and ΔE value in shrimps treated with P₂Mo₁₇Ni, and the inhibitory effect was significant. P₂Mo₁₇Ni could significantly inhibit the increase of total number of colonies, pH value and TVB-N value. P₂Mo₁₇Ni could extend the shelf life of shrimps for 4~5 days. Conclusion: P₂Mo₁₇Ni can slow down the blackening of shrimps and prevent its spoilage. P₂Mo₁₇Ni possesses a good preservation effect on L. vannamei and can be used in the preservation industry of aquatic products.

Publication Date

4-25-2023

First Page

114

Last Page

121

DOI

10.13652/j.spjx.1003.5788.2022.90248

Recommended Citation

Jia-qi, FANG; Qing, HAN; Yang-yu, MA; Fang, WANG; Li-li, LI; Wen-jie, LI; and Li, WANG (2023) "Evaluation of inhibitory activity of a polyoxometallate on polyphenol oxidase and its application in the preservation of Litopenaeus vannamei," Food and Machinery: Vol. 39: Iss. 3, Article 20.
DOI: 10.13652/j.spjx.1003.5788.2022.90248
Available at: https://www.ifoodmm.cn/journal/vol39/iss3/20

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