Screening and identification of extracellular polysaccharide-producing lactic acid bacteria

Authors

CHEN Jing, College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
ZHOU Jia-hao, College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
MAO Qi-qi, College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
TANG Xia, Huangshi Group Hunan Youshi Dairy Co., Ltd., Changsha, Hunan 410008, China
LIU Cheng-guo, College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China; Changsha Innovation Institute for Food of Hunan Agricultural University, Changsha, Hunan 410128, China
ZHOU Hui, College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China; Changsha Innovation Institute for Food of Hunan Agricultural University, Changsha, Hunan 410128, ChinaFollow

Corresponding Author(s)

周辉（1980—），男，湖南农业大学副教授，博士。E-mail:paradise917@163.com

Abstract

Objective: To screen lactic acid bacteria strains with strong ability to produce extracellular polysaccharide. Methods: Forty strains of lactic acid bacteria isolated and preserved in the laboratory were selected, and the commercial strain L. rhamnosus GG （LGG） was used as the positive control strain. The strains with high extracellular polysaccharide yield were screened by colony drawing method and phenol-sulfuric acid method, and their stress resistance, safety and antibiotic sensitivity were tested in vitro. The phenotypic characteristics and species identification of the finally obtained strains were analyzed. Results: After primary screening and secondary screening of 40 strains of lactic acid bacteria, 10 strains of lactic acid bacteria with high extracellular polysaccharide production were obtained. After comprehensive evaluation of polysaccharide production, stress resistance and preliminary safety of the 10 strains of lactic acid bacteria, 4 strains of lactic acid bacteria with high EPS production were finally isolated and identified, namely, 2 strains of lactobacillus paracasei （LZ9089, LZ9Y10）, 1 strains of Lactobacillus casei （LZ9183） and 1 strains of Lactobacillus brevis （LZ9285）. Conclusion: Four strains with high exopolysaccharide yield have good basic probiotic characteristics and have potential development and utilization value.

Publication Date

6-5-2023

First Page

26

Last Page

31,169

DOI

10.13652/j.spjx.1003.5788.2022.81091

Recommended Citation

Jing, CHEN; Jia-hao, ZHOU; Qi-qi, MAO; Xia, TANG; Cheng-guo, LIU; and Hui, ZHOU (2023) "Screening and identification of extracellular polysaccharide-producing lactic acid bacteria," Food and Machinery: Vol. 39: Iss. 4, Article 5.
DOI: 10.13652/j.spjx.1003.5788.2022.81091
Available at: https://www.ifoodmm.cn/journal/vol39/iss4/5

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