Abstract
Objective: The feasibility of the determination of glutamate purity was studied by differential scanning calorimetry. Methods: Indium and Zinc were used to correct the instrument parameters. Under the conditions of nitrogen atmosphere and flow rate of 50 mL/min, the effects of sample particle size, experimental atmosphere, heating rate and sample quality on the DSC measurement results were investigated, and the glutamic acid purity was determined under the optimal conditions. The determination results were compared between DSC and China Pharmacopeia 2015 titration. Results: Under the condition of weighing 3 mg and heating rate at 40 ℃/min, the purity of glutamic acid was 99.74% and the precision was 0.07%. The purity of glutamic acid measured by titration 99.72%. Conclusion: The DSC method has high accuracy and simple operation, and can be used for the determination of glutamate purity.
Publication Date
6-5-2023
First Page
51
Last Page
54,102
DOI
10.13652/j.spjx.1003.5788.2022.80414
Recommended Citation
Ying-nan, MA; Yu-pu, LI; Bei-lei, LIU; Yu-song, MA; Chun-yi, YAO; and Lan-jing, HOU
(2023)
"Purity determination of glutamic acid by DSC,"
Food and Machinery: Vol. 39:
Iss.
4, Article 9.
DOI: 10.13652/j.spjx.1003.5788.2022.80414
Available at:
https://www.ifoodmm.cn/journal/vol39/iss4/9
References
[1] 王万卷, 余巧玲, 潘永红, 等. 基于差示扫描量热法测定硬聚氯乙烯管材微晶熔融焓的研究[J]. 塑料工业, 2020, 48（7）: 95-98.
[2] 王雪蓉, 姚凯, 刘运传, 等. 基于差示扫描量热法的PBT非等温结晶行为研究[J]. 合成树脂及塑料, 2019, 36（6）: 66-70.
[3] 尹洪超, 向凤仙, 段林林, 等. 差示扫描量热法测定渤海原油蜡含量[J]. 化学工程与装备, 2019（10）: 283-285.
[4] 赵欣欣, 杜洪振, 李龙祥, 等. 差示扫描量热技术研究甘油二酯的热力学性质[J]. 食品研究与开发, 2018, 39（6）: 204-208.
[5] 常伟伟, 彭辉. 差示扫描量热法测定对乙酰氨基酚原料药纯度[J]. 化学分析计量, 2020, 29（2）: 36-39.
[6] 翟铁伟. 差示扫描量热法在对照品纯度分析中的应用：以大环内酯类抗生素为例[J].实用药物与临床, 2019, 22（11）: 1 178-1 181.
[7] 张晓燕, 吴子健, 陆健康, 等. 羊肝蛋白稳定性及NaCl质量分数对其功能特性的影响[J]. 食品与机械, 2021, 37（3）: 1-6.
[8] 张丹丹, 刘晓康, 马丽苹, 等. 红薯/玉米淀粉共混物糊、流变及热力学性质研究[J]. 食品与机械, 2019, 35（4）: 31-36.
[9] 王进, 王刚, 罗丽娟, 等. 茚三酮比色法测定聚谷氨酸含量的研究[J]. 食品与发酵科技, 2019, 55（2）: 98-101.
[10] 国家药典委员会. 中国药典: 2015版: 二部[M]. 北京: 中国医药科技出版社, 2015: 509-510.
[11] 祝洪艳, 张荻, 张力娜, 等. 柱前衍生化HPLC法检测紫苏子和紫苏叶中氨基酸的含量[J]. 药物分析杂志, 2017, 37（10）: 1 858-1 864.
[12] 黄元礼, 柯林楠, 李常坤, 等. 液相质谱联用法同时检测体外生殖培养液中的18种氨基酸[J]. 中国组织工程研究, 2018, 22（18）: 2 915-2 920.
[13] 陈青, 魏伯荣, 包德君. 差示扫描量热法单峰测定物质的纯度[J]. 分析仪器, 2005（3）: 42-46.
[14] 池海涛, 刘伟丽, 高峡, 等. 差示扫描量热法及其发展趋势[J]. 食品安全质量检测学报, 2016, 7（11）: 4 374-4 377.
[15] 李承花, 张奕, 左琴华, 等. 差式扫描量热仪的原理与应用[J]. 分析仪器, 2015（4）: 88-94.
[16] 范利静. 影响差示扫描量热仪测试的因素[J]. 上海塑料, 2016（3）: 27-31.
[17] 苏小琴, 龙伟, 刘秀兰, 等. 差示扫描量热仪的影响因素及测试技术[J]. 分析仪器, 2019（4）: 74-79.
[18] 黄钰香, 庞承焕, 林木良. DSC检测过程影响因素的探讨研究[J]. 广州化工, 2008, 36（5）: 1-6.
[19] 王彦飞, 王婧莹, 李亚楠, 等. DSC法测定2,3,6-三甲基苯酚的纯度及其热力学性质[J]. 天津科技大学学报, 2018, 33（6）: 41-44.
[20] 沈紧治, 王政, 苏玉纯. 差示扫描量热法对5种硫酸盐类矿物药的分析鉴别研究[J]. 中国医院用药评价与分析, 2019, 19（6）: 717-720.