Optimization of extraction technology and determination of sodium hyaluronate in solid drinks by ethanol precipitation combined with enzymatic hydrolysis- reversed phase chromatography

Authors

ZHANG Zhi-zhou, Institute of Analysis, Guangdong Academy of Sciences 〔China National Analytical Center, Guangzhou〕, Guangzhou, Guangdong 510070, China; Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Guangzhou, Guangdong 510070, China; Guangdong Provincial Engineering Research Center for Efficacy Component Testing and Risk Substance Rapid Screening of Health Food, Guangzhou, Guangdong 510070, China
LIN Jia-ting, Institute of Analysis, Guangdong Academy of Sciences 〔China National Analytical Center, Guangzhou〕, Guangzhou, Guangdong 510070, China; Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Guangzhou, Guangdong 510070, China; Guangdong Provincial Engineering Research Center for Efficacy Component Testing and Risk Substance Rapid Screening of Health Food, Guangzhou, Guangdong 510070, ChinaFollow
PAN Can-sheng, Institute of Analysis, Guangdong Academy of Sciences 〔China National Analytical Center, Guangzhou〕, Guangzhou, Guangdong 510070, China; Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Guangzhou, Guangdong 510070, China; Guangdong Provincial Engineering Research Center for Efficacy Component Testing and Risk Substance Rapid Screening of Health Food, Guangzhou, Guangdong 510070, China
ZHANG Fei, Institute of Analysis, Guangdong Academy of Sciences 〔China National Analytical Center, Guangzhou〕, Guangzhou, Guangdong 510070, China; Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Guangzhou, Guangdong 510070, China; Guangdong Provincial Engineering Research Center for Efficacy Component Testing and Risk Substance Rapid Screening of Health Food, Guangzhou, Guangdong 510070, China
HU Han-han, Institute of Analysis, Guangdong Academy of Sciences 〔China National Analytical Center, Guangzhou〕, Guangzhou, Guangdong 510070, China; Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Guangzhou, Guangdong 510070, China; Guangdong Provincial Engineering Research Center for Efficacy Component Testing and Risk Substance Rapid Screening of Health Food, Guangzhou, Guangdong 510070, China

Corresponding Author(s)

林嘉婷（1984—），女，广东省科学院测试分析研究所（中国广州分析测试中心）助理工程师，学士。E-mail：236249624@qq.com

Abstract

Objective: This study aimed to establish the methods for determining the content of sodium hyaluronate in solid drinks by reversed-phase high performance liquid chromatography. Methods: With sodium hyaluronate content as evaluation index, after the sample extract was precipitated by ethanol, hyaluronase was used to hydrolyze the precipitate. The optimal enzymolysis conditions obtained by single factor test and orthogonal test were as follows: enzymolysis, at 34 ℃ and pH 6.5, for 120 min. The determination was performed on Uliumate C₁₈ (4.6 mm×250 mm,5 μm) column with mobile phase: acetonitrile 10 mmol/L sodium octane sulfonate solution (V_acetonitrile∶V_{octane sulfonate} =10∶90) at flow rate of 0.6 mL/min. The detection wavelength was 232 nm and quantified by external standard method. Results: The correlation coefficient of sodium hyaluronate was 0.999 9. The recoveries of samples were between 96.1%~101%, and the relative standard deviation was 3.16%. The determination of limit was 0.062 g/kg. Conclusion: Ethanol precipitation combined with enzymolysis-reversed phase chromatography has strong specificity, high accuracy, good repeatability and less interference, which is suitable for the determination of sodium hyaluronate in solid drinks.

Publication Date

6-9-2023

First Page

64

Last Page

69

DOI

10.13652/j.spjx.1003.5788.2022.80870

Recommended Citation

Zhi-zhou, ZHANG; Jia-ting, LIN; Can-sheng, PAN; Fei, ZHANG; and Han-han, HU (2023) "Optimization of extraction technology and determination of sodium hyaluronate in solid drinks by ethanol precipitation combined with enzymatic hydrolysis- reversed phase chromatography," Food and Machinery: Vol. 39: Iss. 5, Article 11.
DOI: 10.13652/j.spjx.1003.5788.2022.80870
Available at: https://www.ifoodmm.cn/journal/vol39/iss5/11

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