Abstract
Cicada flower is a valuable edible and medicinal mushroom. Polysaccharides are the most important active ingredients of cicada flower. This paper reviewed the extraction, separation and purification technologies of cicada flower polysaccharides in recent years. Also, it analyzed the biological activities of cicada flower polysaccharides, such as antioxidant, anti-tumor, immune regulation, bacteriostasis, hypolipidemic, hypoglycemic, anti-virus and ease pain. Moreover, the development and application of products were summarized and the existing problems and future research directions were also discussed.
Publication Date
6-9-2023
First Page
217
Last Page
223,240
DOI
10.13652/j.spjx.1003.5788.2023.80083
Recommended Citation
Guo-jun, JIA; Xiao-ying, WANG; Jian-min, YUN; and Jian-fei, DONG
(2023)
"Research progress on extraction, biological activities and product development of polysaccharides from cicada flower,"
Food and Machinery: Vol. 39:
Iss.
5, Article 34.
DOI: 10.13652/j.spjx.1003.5788.2023.80083
Available at:
https://www.ifoodmm.cn/journal/vol39/iss5/34
References
[1] 李增智, HYWEL-JONES N L, 栾丰刚, 等. 与蝉花有关的虫草菌生物多样性的研究I: 文献考证[J]. 菌物学报, 2020, 39(12): 2 191-2 201.
LI Z Z, HYWEL-JONES N L, LUAN F G, et al. Biodiversity of cordycipitoid fungi associated with Isaria cicadae I: Literature study[J]. Mycosystema, 2020, 39(12): 2 191-2 201.
[2] 李增智, 栾丰刚, HYWEL-JONES N L, 等. 与蝉花有关的虫草菌生物多样性的研究II: 重要药用真菌蝉花有性型的发现及命名[J]. 菌物学报, 2021, 40(1): 95-107.
LI Z Z, LUAN F G, HYWEL-JONES N L, et al. Biodiversity of cordycipitoid fungi associated with Isaria cicadae Miquel II: Teleomorph discovery and nomenclature of chanhua, an important medicinal fungus in China[J]. Mycosystema, 2021, 40(1): 95-107.
[3] 温鲁, 唐玉玲, 张平. 蝉花与有关虫草活性成分检测比较[J]. 江苏中医药, 2006(1): 45-46.
WEN L,TANG Y L, ZHANG P. Comparison of detection of active components between Cordyceps sobolifera and related Cordyceps[J]. Jiangsu Journal of Traditional Chinese Medicine, 2006(1): 45-46.
[4] 许胜杰, 叶淑幸, 陈劲初. 新资源食品蝉花菌丝体的开发[J]. 食药用菌, 2011, 19(6): 34-37.
XU S J, YE S X, CHEN J C. Development of new resource food of mycelium from cicada flower[J]. Edible and Medicinal Mushrooms, 2011, 19(6): 34-37.
[5] 张胜利, 蒲顺昌, 栾丰刚, 等. 一株蝉花虫草菌重寄生真菌的分离鉴定[J]. 菌物研究, 2021, 19(1): 29-35.
ZHANG S L, PU S C, LUAN F G, et al. Identification of a mycoparasite Isolated from Cordyceps chanhua[J]. Journal of Fungal Research, 2021, 19(1): 29-35.
[6] 李挺, 宋斌, 林群英. 蝉花的研究进展[J]. 中药材, 2008(9): 1 443-1 448.
LI T, SONG B, LIN Q Y. Research progress of cicada flower[J]. Journal of Chinese Medicinal Materials, 2008(9): 1 443-1 448.
[7] ZHAO J, XIE J, WANG L Y, et al. Advanced development in chemical analysis of Cordyceps[J]. Journal of Pharmaceutical and Biomedical Analysis, 2014, 87: 271-289.
[8] MENG C, HAN Q, WANG X, et al. Determination and quantitative comparison of nucleosides in two Cordyceps by HPLC-ESI-MS-MS[J]. Journal of Chromatographic Science, 2019, 57(5): 426-433.
[9] 屈可伸. 蝉花的中药药理研究进展[J]. 健康之路, 2016, 15(9): 25.
QU K S. Progress in pharmacological research of cicada flower[J]. Health Way, 2016, 15(9): 25.
[10] 陈惠. 食用菌与健康[M]. 上海: 上海科学普及出版社, 2021: 254-260.
CHEN H. Edible mushrooms and health[M]. Shanghai: Shanghai Science Popularization Press, 2021: 254-260.
[11] 葛飞, 夏成润, 李春如, 等. 蝉拟青霉菌丝体与天然蝉花中化学成分的比较分析[J]. 菌物学报, 2007(1): 68-75.
GE F, XIA C R, LI C R, et al. Analysis of the chemical compositions of Paecilomyces cicadae fermented mycelia and Cordyceps cicadae fruit body[J]. Mycosystema, 2007(1): 68-75.
[12] KIM H S, KIM J Y, RYU H S, et al. Phenotypic and functional maturation of dendritic cells induced by polysaccharide isolated from Paecilomyces cicadae[J]. Journal of Medicinal Food, 2011, 14: 847-856.
[13] ZHANG Y, WU Y T, ZHENG W, et al. The antibacterial activity and antibacterial mechanism of a polysaccharide from Cordyceps cicadae[J]. Journal of Functional Foods, 2017, 38: 273-279.
[14] 蒋宁, 高大伟, 林金盛, 等. 蝉花的研究进展[J]. 江苏农业科学, 2017, 45(8): 11-14.
JIANG N, GAO D W, LIN J S, et al. Research progress of cicada flower[J]. Jiangsu Agricultural Sciences, 2017, 45(8): 11-14.
[15] 郭文场, 胡振东, 刘佳贺. 药食兼用虫草菌——蝉花[J]. 特种经济动植物, 2017, 20(11): 39-45.
GUO W C, HU Z D, LIU J H. Edible and medicinal cicada flower[J]. Special Economic Animals and Plants, 2017, 20(11): 39-45.
[16] 秦文平, 慕程, 殷世鹏. 中药蝉花的化学成分及药理作用研究进展[J]. 中国中医药图书情报杂志, 2019, 43(4): 73-76.
QIN W P, MU C, YIN S P. Research progress of chemical components and pharmacological effects of Isaria cicadae miquel[J]. Chinese Journal of Library and information Science for Traditional Chinese Medicine, 2019, 43(4): 73-76.
[17] 李建平, 张铁, 曾文波. 蝉花虫草分类地位及其遗传多样性研究进展[J]. 中国食用菌, 2019, 38(11): 1-5, 16.
LI J P, ZHANG T, ZENG W B. Research progresses on taxonomic status and genetic diversity of Isaria cicadae[J]. Edible Fungi of China, 2019, 38(11): 1-5, 16.
[18] 李龙宇, 葛言琪, 伍一炜, 等. 蝉花多糖的研究进展[J]. 现代中药研究与实践, 2019, 33(4): 83-86.
LI L Y, GE Y Q, WU Y W, et al. Research progress on polysaccharides from Cordyceps cicadae[J]. Research and Practice on Chinese Medicines, 2019, 33(4): 83-86.
[19] 耿晓进, 李海清, 刘紫征. 食药用菌多糖提取工艺研究进展[J]. 食用菌, 2019, 41(6): 1-5.
GENG X J, LI H Q, LIU Z Z. Research progress in extraction technology of polysaccharides from edible and medicinal mushrooms[J]. Edible Fungi, 2019, 41(6): 1-5.
[20] 鲁吉珂, 古国峰, 汪瑞, 等. 蝉花多糖提取工艺优化研究[J]. 食品工业科技, 2013, 34(22): 196-199.
LU J K, JI G F, WANG R, et al. Optimization of extraction of polysaccharide from Cordyceps sobolifera[J]. Science and Technology of Food Industry, 2013, 34(22): 196-199.
[21] 于士军, 包佳源, 樊美珍. 人工蝉花孢梗束粗多糖的提取工艺和活性[J]. 菌物研究, 2014, 12(2): 100-106.
YU S J, BAO J Y, FAN M Z. Extraction technology and biological activities of crude polysaccharide from synnemata of Cordyceps cicadae culture[J]. Journal of Fungal Research, 2014, 12(2): 100-106.
[22] 岑绮雯, 宋宇婧, 陈涛, 等. 蝉花多糖提取工艺优化及抗菌活性初步研究[J]. 杭州师范大学学报(自然科学版), 2018, 17(3): 269-274.
CEN Q W, SONG Y J, CHEN T, et al. Optimization of extraction technology for polysaccharide from Cordyceps cicadae and the preliminary investigation of its antibacterial activity[J]. Journal of Hangzhou Normal University (Natural Science Edition), 2018, 17(3): 269-274.
[23] 彭瑛, 郭丹钊, 刘任糠, 等. 超声波辅助蝉花多糖提取工艺优化研究[J]. 轻工科技, 2017, 33(6): 16-17, 114.
PENG Y, GUO D Z, LIU R K, et al. Optimization of ultrasonic-assisted extraction process of polysaccharide from cicada flower[J]. Light Industry Science and Technology, 2017, 33(6): 16-17, 114.
[24] 黄小忠, 高大响, 张雪松, 等. 蝉花多糖提取工艺的优化[J]. 浙江农业科学, 2019, 60(8): 1 385-1 387.
HUANG X Z, GAO D X, ZHANG X S, et al. Optimization of extraction process of polysaccharide from cicada flower[J]. Journal of Zhejiang Agricultural Sciences, 2019, 60(8): 1 385-1 387.
[25] 凡军民, 谢正林, 谢春芹, 等. 超声波辅助双水相体系提取蝉花多糖及抗氧化研究[J]. 食品科技, 2020, 45(5): 196-201.
FAN J M, XIE Z L, XIE C Q, et al. Optimization of ultrasonic-assisted aqueous two-phase extraction and antioxidant activity of polysaccharide from Cordyceps cicadae[J]. Food Science and Technology, 2020, 45(5): 196-201.
[26] 姚婷, 罗靖, 宋捷民. 金蝉花多糖的微波辅助提取工艺研究[J]. 江西中医学院学报, 2012, 24(5): 73-76.
YAO T, LUO J, SONG J M. Study on microwave-assisted extraction of polysaccharides in Cordyceps cicadae[J]. Journal of Jiangxi University of Chinese Medicine, 2012, 24(5): 73-76.
[27] 熊中奎, 金丽琴, 吕建新. 利用正交试验优化蝉拟青霉多糖的提取工艺[J]. 温州医学院学报, 2006(5): 422-423.
XIONG Z K, JIN L Q,LU J X. Optimization of processing technique of polysaccharide from Paecilomyces cicadae[J]. Journal of Wenzhou Medical College, 2006(5): 422-423.
[28] 刘芸, 谭艾娟, 吕世明. 酶法提取蝉拟青霉多糖的研究[J]. 天津农业科学, 2010, 16(2): 36-38.
LIU Y, TAN A J, LU S M. Study on the enzyme-extraction method of polysaccharide from Paecilomyce cicadae[J]. Tianjin Agricultural Sciences, 2010, 16(2): 36-38.
[29] 史世晨. 蝉花多糖提取工艺优化及活性研究[D]. 合肥: 安徽农业大学, 2015: 49.
SHI S C. Study on the optimal extraction process and the activity of Cordyceps cicadae polysaccharide[D]. Hefei: Anhui Agricultural University, 2015: 49.
[30] 宋佳敏, 王鸿飞, 孙朦, 等. 响应面法优化金蝉花多糖提取工艺及抗氧化活性分析[J]. 食品科学, 2018, 39(4): 275-281.
SONG J M, WANG H F, SUN M, et al. Optimizationof extraction and antioxidant activity of polysaccharides from Cordyceps cicadae by response surface methodology[J]. Food Science, 2018, 39(4): 275-281.
[31] 梁康怡, 张华林. 响应面法优化蝉花多糖超声波辅助提取工艺[J]. 广东化工, 2020, 47(21): 38-40.
LIANG K Y, ZHANG H L. Optimization of ultrasonic-assisted extraction process of polysaccharide from Cordyceps cicadae by response surface[J]. Guangdong Chemical Industry, 2020, 47(21): 38-40.
[32] 南婷婷, 许东霞, 王余宸铭, 等. 蝉花孢子粉多糖的酶辅助提取及其对酒精性肝损伤小鼠的保护作用[J]. 食品工业科技, 2021, 42(2): 295-301, 309.
NAN T T, XU D X,WANG Y C M, et al. Enzyme-assisted extraction of polysaccharides from Cordyceps cicadae spores and its hepatoprotective effect against alcohol-induced liver injury in mice[J]. Science and Technology of Food Industry, 2021, 42(2): 295-301, 309.
[33] 邵双宇, 司夏利, 张岩松, 等. 食用菌多糖分析方法研究进展[J]. 食品科学, 2020, 41(19): 272-280.
SHAO S Y,SI X L, ZHANG Y S, et al. Recent advances in analytical methods for polysaccharides from edible mushroom[J]. Food Science, 2020, 41(19): 272-280.
[34] 孙小梅. 灵芝子实体多糖色谱指纹分析方法的研究[D]. 无锡: 江南大学, 2014: 4-5.
SUN X M. Study on chromatographic fingerprint analysis methods of polysaccharides from Ganoderma lucidum fruiting bodies[D]. Wuxi: Jiangnan University, 2014: 4-5.
[35] 吴小燕. 黑木耳多糖提取、分离纯化及体外抗凝血活性[D]. 芜湖: 安徽工程大学, 2016: 3-5.
WU X Y. Study on isolation, purification and anticoagulant activity of Auricularia auricula polysaccharides[D]. Wuhu: Anhui Polytechnic University, 2016: 3-5.
[36] 冉靓, 周婵媛, 张桂玲, 等. 黑虎掌菌多糖脱色工艺及其抗氧化活性[J]. 食品工业, 2020, 41(5): 170-174.
RAN L, ZHOU C Y, ZHANG G L, et al. Decolorization process and antioxidant activity of polysaccharide from Sarcodon aspratum[J]. The Food Industry, 2020, 41(5): 170-174.
[37] WU D T, XIE J, WANG L Y, et al. Characterization of bioactive polysaccharides from Cordyceps militaris produced in China using saccharide mapping[J]. Journal of Functional Foods, 2014, 9: 315-323.
[38] RUTHES A C, SMIDERLE F R, IACOMMINI M. D-glucans from edible mushrooms: A review on the extraction, purification and chemical characterization approaches[J]. Carbohydrate Polymers, 2015, 117: 753-761.
[39] 周俐斐, 芦柏震, 蔡菊芬. 蝉花总多糖的提取纯化及含量测定[J]. 江西中医药, 2009, 40(1): 56-57.
ZHOU L F, LU B Z, CAI J F. Extraction, purification and content determination of total polysaccharide from cicada flower[J]. Jiangxi Journal of Traditional Chinese Medicine, 2009, 40(1): 56-57.
[40] 徐娇. 蚕蛹蝉花多糖的理化特性及抗氧化、抗肿瘤功能研究[D]. 镇江: 江苏科技大学, 2021: 47.
XU J. Physicochemicalproperties, antioxidant, antitumor effects of polysaccharide from silkworm chrysalis Cordyceps cicadae [D]. Zhenjiang: Jiangsu University of Science and Technology, 2021: 47.
[41] 封燕, 贡小辉, 韦德群, 等. 金蝉花多糖的抗氧化活性及结构分析[J]. 食品科学, 2016, 37(13): 19-24.
FENG Y, GONG X H, WEI D Q, et al. Antioxidant activity and preliminary structure analysis of polysaccharides from Cordyceps cicadas[J]. Food Science, 2016, 37(13): 19-24.
[42] 邵颖, 陈安徽, 赵节昌, 等. 人工培育蝉花孢梗束分级多糖的抗氧化活性及对果蝇寿命的影响[J]. 食品研究与开发, 2018, 39(14): 187-192.
SHAO Y, CHEN A H, ZHAO J C, et al. Effect of rating polysaccharides from artificial fruiting body of Cordyceps cicadae on lifespan of Drosophila melanogaster and the antioxidant activity[J]. Food Research and Development, 2018, 39(14): 187-192.
[43] 芦柏震, 姜志明, 牟翰舟, 等. 蝉花粗提物对肺癌细胞作用的实验研究[J]. 中国中医药科技, 2006(5): 328-329.
LU B Z, JIANG Z M, MOU H Z, et al. Experimental study on the effect of Cicada Flower extract on lung cancer cells[J]. Chinese Journal of Traditional Medical Science and Technology, 2006(5): 328-329.
[44] 胡云良, 杨介钻, 金丽琴, 等. 蝉拟青霉总多糖激活淋巴细胞作用及对白血病细胞的增殖抑制作用[J]. 中华血液学杂志, 2007, 28(6): 413-415.
HU Y L, YANG J Z, JIN L Q, et al. Activation of lymphocytes and inhibition of proliferation of leukemic cells by total polysaccharide from Paecilomyces cicadae[J]. Chinese Journal of Hematology, 2007, 28(6): 413-415.
[45] 蔡菊芬, 姜志明, 卢红阳, 等. 蝉拟青霉不同纯化组分对体外抗肿瘤作用的基础研究[J]. 中华中医药学刊, 2010, 28(4): 760-764.
CAI J F, JIANG Z M, LU H Y, et al. Research of anti-tumor effects of different purified components of Cordyceps cicadae in vitro[J]. Chinese Archives of Traditional Chinese Medicine, 2010, 28(4): 760-764.
[46] 卓佳, 杨介钻, 金丽琴, 等. 蝉拟青霉多糖体外对乙型肝炎病毒复制的抑制作用[J]. 中国病理生理杂志, 2010, 26(2): 327-332.
ZHUO J, YANG J Z, JIN L Q, et al. Effect of Paecilomyces cicadae polysaccharide on duplication of hepatitis B virus in HepG2.2.15 cell strain[J]. Chinese Journal of Pathophysiology, 2010, 26(2): 327-332.
[47] 谢飞, 李伟, 陈美珍, 等. 野生蝉花多糖抗肿瘤活性及其作用机制[J]. 食品科学, 2016, 37(13): 209-213.
XIE F, LI W, CHEN M Z, et al. Antitumor activity and mechanism of action of wild Cordyceps sobolifera polysaccharide[J]. Food Science, 2016, 37(13): 209-213.
[48] 杨全伟, 肖柳, 黄雄超, 等. 金蝉花多糖的含量组成分析及其对LPS诱导的THP-1细胞NF-κB活性的研究[J]. 中国现代中药, 2016, 18(9): 1 129-1 134.
YANG Q W, XIAO L, HUANG X C, et al. Polysaccharide content and composition analysis of golden cicada flowers and effect on NF-κB activity of LPS inducted THP-1 cells[J]. Modern Chinese Medicine, 2016, 18(9): 1 129-1 134.
[49] 何晓波, 芦柏震, 周俐斐, 等. 蝉花总多糖对细胞免疫和体液免疫反应的促进作用[J]. 中华中医药学刊, 2010, 28(7): 1 465-1 468.
HE X B, LU B Z, ZHOU L F, et al. The promotion effects of total polysaccharide from Cordyceps cicadae on cellular and humoral immune responses[J]. Chinese Archives of Traditional Chinese Medicine, 2010, 28(7): 1 465-1 468.
[50] 封燕. 金蝉花多糖的结构特征及免疫活性初步研究[D]. 镇江: 江苏大学, 2016: 53-55.
FENG Y. Structural characterization and immunomodulatory effects of a polysaccharide isolated from Cordyceps cicadae[D]. Zhenjiang: Jiangsu University, 2016: 53-55.
[51] 宋佳敏, 王鸿飞, 罗洁, 等. 金蝉花多糖对小鼠免疫功能的影响[J]. 核农学报, 2018, 32(10): 1 977-1 983.
SONG J M, WANG H F, LUO J, et al. Effect of Cordyceps cicadae polysaccharides on immunologic function of mice[J]. Journal of Nuclear Agricultural Sciences, 2018, 32(10): 1 977-1 983.
[52] 李瑞萍, 刘长国, 董丽艳, 等. 蝉花多糖和植物提取物对雁荡麻鸡生长性能和免疫功能的影响[J]. 动物营养学报, 2020, 32(11): 5 433-5 440.
LI R P, LIU Z G, DONG L Y, et al. Effects of Cordyceps cicadae polysaccharide and plant extract on growth performance and immune function of Yandang Ma-chicken[J]. Chinese Journal of Animal Nutrition, 2020, 32(11): 5 433-5 440.
[53] 孙寒雪, 刘雅丽, 曾涛, 等. 包被丁酸钠或蝉花多糖对乳鸽生长性能、屠宰性能及血清抗氧化和免疫指标的影响[J]. 动物营养学报, 2021, 33(10): 5 645-5 652.
SUN H X, LIU Y L, ZENG T, et al. Effects of coated sodium butyrate or Cordyceps cicadae polysaccharide on growth gerformance, slaughter performance and serum antioxidant and immune indexes of squabs[J]. Chinese Journal of Animal Nutrition, 2021, 33(10): 5 645-5 652.
[54] 刘素贞, 李瑞萍, 刘长国, 等. 人工蝉花多糖对三黄鸡免疫功能及部分相关因子基因表达的影响[J]. 畜牧与兽医, 2021, 53(5): 83-87.
LIU S Z, LI R P, LIU Z G, et al. Effect of polysaccharides from Cordyceps cicadae on immune function and molecular expression in Sanhuang chicken[J]. Animal Husbandry & Veterinary Medicine, 2021, 53(5): 83-87.
[55] 翁梁. 野生蝉花与人工蝉花多糖的提取及抑菌试验[J]. 农业科技与装备, 2010(11): 22-24, 29.
WENG L. Study on the extraction of polysaccharide from wild and artificial Cordyceps sobolifera and bacteriostasis effect[J]. Agricultural Science & Technology and Equipment, 2010(11): 22-24, 29.
[56] 陈成, 许佳伟, 孙细涓, 等. 茅山地区蝉花菌株的分离及其多糖生物活性的研究[J]. 江苏农业科学, 2015, 43(4): 347-352.
CHEN C, XU J W, SUN X J, et al. Isolation of Ophiocordyceps sobolifera strain from Maoshan area and study on its polysaccharide biological activity[J]. Jiangsu Agricultural Sciences, 2015, 43(4): 347-352.
[57] 杨介钻, 金丽琴, 吕建新, 等. 蝉拟青霉多糖抗衰老作用的实验研究[J]. 中国老年学杂志, 2004(4): 343-344.
YANG J Z, JIN L Q, LU J X, et al. The experimental study of Paecilomyces cicadicae polysaccharides on anti-aging[J]. Chinese Journal of Gerontology, 2004(4): 343-344.
[58] 宋捷民, 忻家础, 朱英. 蝉花对小鼠血糖及造血功能影响[J]. 中华中医药学刊, 2007(6): 1 144-1 145.
SONG J M,XIN J C, ZHU Y. Effect of Cordyceps cicadae on blood sugar of mice and its hematopoietic function[J]. Chinese Archives of Traditional Chinese Medicine, 2007(6): 1 144-1 145.
[59] 艾仁丽, 陈波利, 谭艾娟, 等. 蝉拟青霉菌丝提取物镇痛作用研究[J]. 现代农业科技, 2018(24): 227, 236.
AI R L, CHEN B L, TAN A J, et al. Study on analgesic effect of extract of Paecilomyces cicadae mycelium[J]. Modern Agricultural Science and Technology, 2018(24): 227, 236.
[60] 赵节昌, 邵颖, 任格, 等.蝉花孢子粉与孢梗束的化学成分及其多糖对果蝇寿命的影响[J]. 食品科技, 2019, 44(4): 205-210, 219.
ZHANG J C, SHAO Y, REN G, et al. The chemical components of spore powder and synnemata of Isaria Cicadae and effect of the polysaccharides on lifespan of Drosphila melanogaster[J]. Food Science and Technology, 2019, 44(4): 205-210, 219.
[61] 孙长胜, 徐振栋, 沈佳奇. 蝉花延寿及抗氧化作用[J]. 食品工业, 2022, 43(10): 156-159.
SUN C S, XU Z D, SHEN J Q. Lifeprolonging and antioxidant effects of Cordyceps chanhua[J]. The Food Industry, 2022, 43(10): 156-159.
[62] 周文川, 唐自钟, 周铭, 等. 一种金蝉花多糖养生饮料及其制备方法: 112741327A[P]. 2021-05-04.
ZHOU W C, TANG Z Z, ZHOU M. A golden cicada flower polysaccharide health drink and its preparation method: 112741327A[P]. 2021-05-04.
[63] 孙晓辉. 一种抗尿酸功能性食品及其制备工艺: 113812613A[P]. 2021-12-21.
SUN X H. An anti uric acid functional food and its preparation process: 113812613A[P]. 2021-12-21.
[64] 李成, 彭国杰, 吴凯强, 等. 一种蝉花菌质啤酒的制备方法: 108117943B[P]. 2021-12-24.
LI C, PENG G J, WU K Q, et al. A preparation method of cicada flower mycoplasm beer: 108117943B[P]. 2021-12-24.
[65] 唐国笔, 唐得俊. 一种蝉花虫草组合物及其制备的蝉花虫草饮料: 116076698A[P]. 2023-05-09.
TANG G B, TANG D J. A cicada flower Cordyceps beverage prepared from cicada flower Cordyceps Composition: 116076698A[P]. 2023-05-09.
[66] CHEN Y L, SHU-HSING Y, LIN T W, et al. A 90-day subchronic toxicity study of submerged mycelial culture of Cordyceps cicadae (Ascomycetes) in rats[J]. International Journal of Medicinal Mushrooms, 2015, 17(8): 771-781.
[67] 解思友, 尹彬, 龙文君, 等. 蝉花及其成分增强免疫、抗肿瘤药理作用研究进展[J]. 药物评价研究, 2020, 43(4): 624-629.
XIE S Y, YIN B, LONG W J, et al. Research progress ofcicada flower and its components in enhancing immunity and anti-tumor pharmacology[J]. Drug Evaluation Research, 2020, 43(4): 624-629.