Pollution status and research progress on the detection technology of Alternaria mycotoxins in foods

Authors

WANG Xia, Shanghai Center of Agri-products Quality and Safety, Shanghai 201708, China
FENG Dong-sheng, Shanghai Center of Agri-products Quality and Safety, Shanghai 201708, China
TONG Jin-rong, Shanghai Center of Agri-products Quality and Safety, Shanghai 201708, China
GAO Meng-feng, Shanghai Center of Agri-products Quality and Safety, Shanghai 201708, China
ZHANG Wei-yi, Shanghai Center of Agri-products Quality and Safety, Shanghai 201708, ChinaFollow

Corresponding Author(s)

张维谊（1979—），女，上海市农产品质量安全中心研究员，硕士。E-mail: zhangharewei@163.com

Abstract

Alternaria mycotoxins are the secondary metabolites of Alternaria species, which have obvious toxicity （mutagenicity, teratogenicity and carcinogenicity, etc） to humans and animals. Alternariol （AOH）, alternariol monomethyl ether （AME）, alternuene （ALT）, tenuazonic acid （TeA） and tenoxin （TEN） are the most studied at present. Alternaria mycotoxins was reported in apples, orange, tomatoes, corns and other agricultural products. Eating these foods contaminated by Alternaria mycotoxins will cause serious harm to human health. In this paper, the pollution status and the detection technology of Alternaria mycotoxins were summarized, and the future research direction of Alternaria mycotoxins was prospected. The purpose was to provide references for the risk assessment, detection and prevention of Alternaria mycotoxins in foods.

Publication Date

6-9-2023

First Page

224

Last Page

231

DOI

10.13652/j.spjx.1003.5788.2022.80542

Recommended Citation

Xia, WANG; Dong-sheng, FENG; Jin-rong, TONG; Meng-feng, GAO; and Wei-yi, ZHANG (2023) "Pollution status and research progress on the detection technology of Alternaria mycotoxins in foods," Food and Machinery: Vol. 39: Iss. 5, Article 35.
DOI: 10.13652/j.spjx.1003.5788.2022.80542
Available at: https://www.ifoodmm.cn/journal/vol39/iss5/35

References

[1] 郭诗曼, 冯启. 链格孢霉菌毒素的研究进展[J]. 广州化工, 2020, 48（17）: 9-12. GUO S M, FENG Q. Research progress on Alternaria toxins[J]. Guangzhou Chemical Industry, 2020, 48（17）: 9-12.
[2] 邢家溧, 张子庚, 郑睿行, 等. 固相萃取—超高效液相色谱—串联质谱法检测婴幼儿奶粉中的7种链格孢霉毒素[J]. 色谱, 2022, 40（2）: 156-164. XING J L, ZHANG Z G, ZHENG R X, et al. Determination of seven Alternaria toxins in infant milk powder by solid phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2022, 40（2）: 156-164.
[3] 何国鑫, 邓青芳, 周欣. 链格孢霉毒素的分析方法及其毒理机制研究进展[J]. 食品工业科技, 2018（2）: 341-352, 356. HE G X, DENG Q F, ZHOU X. Research progress of analytical methods and toxicological mechanisms for Alternaria mycotoxins[J]. Science and Technology of Food Industry, 2018（2）: 341-352, 356.
[4] NGUYEN THUONG T T, KIM J, JEON S J, et al. Mycotoxin production of Alternaria strains isolated from Korean barley grains determined by LC-MS/MS[J]. International Journal of Food Microbiology, 2018, 268: 44-52.
[5] 乔晓婷. 中国人群总膳食中链格孢霉毒素暴露水平研究[D]. 南昌: 南昌大学, 2020: 1. QIAO X T. Study on the exposure levels of Alternaria mycotoxins based on the Chinese total diets[D]. Nanchang: Nanchang University, 2020: 1.
[6] SOLFRIZZO M. Recent advances on Alternaria mycotoxins[J]. Current Opinion in Food Science, 2017, 17: 57-61.
[7] 吴希, 邢家溧, 郑睿行, 等. 超高效液相色谱串联质谱法快速检测麦类中典型链格孢霉毒素[J]. 食品科学, 2022, 43（12）: 317-324. WU X, XING J L, ZHENG R X, et al. Rapid determination of typical Alternaria toxins in wheat by ultra performance liquid chromatography-tandem mass spectrometry[J]. Food Science, 2022, 43（12）: 317-324.
[8] 何玲, 秦忠雪, 任琳, 等. 四川省市售小麦及其制品中链格孢霉毒素污染况调查[J]. 预防医学情报杂志, 2020, 36（11）: 1 433-1 437. HE L, QIN Z X, REN L, et al. Investigation on contamination of Alternaria toxins from wheat and its products sold in Sichuan province[J]. Journal of Preventive Medicine Information, 2020, 36（11）: 1 433-1 437.
[9] 薛华丽, 毕阳, 宗元元, 等. 果蔬及其制品中真菌毒素的污染与检测研究进展[J]. 食品科学, 2016, 37（23）: 285-290. XUE H L, BI Y, ZONG Y Y, et al. Progress in mycotoxins contamination and detection in fruits and vegetables and their processed products[J]. Food Science, 2016, 37（23）: 285-290.
[10] 蒋黎艳. 柑橘中5种链格孢霉毒素检测技术及产生分布规律初探[D]. 重庆: 西南大学, 2016. JIANG L Y. Preliminary studies on the detection technology and distribution of five Alternaria mycotoxins pollution in citrus[D]. Chongqing: Southwest University, 2016.
[11] 张子庚, 张爱芝, 邢家溧, 等. 高效液相色谱—串联质谱法测定芒果中六种链格孢霉毒素残留[J]. 食品工业科技, 2020, 41（20）: 199-211. ZHANG Z G, ZHANG A Z, XING J L, et al. Determination residues of six Alternaria toxins in mango by HPLC-MS[J]. Science and Technology of Food Industry, 2020, 41（20）: 199-211.
[12] FAN C, CAO X L LIU M, et al. Determination of Alternaria mycotoxins in wine and juice using ionic liquid modified countercurrent chromatography as a pretreatment method followed by high-performance liquid chromatography[J]. Journal of Chromatography A, 2016, 1 436: 133-140.
[13] 张晓男, 聂继云, 闫震, 等. UPLC-MS/MS同时检测苹果及其制品中的7种真菌毒素[J]. 分析测试学报, 2017, 36（5）: 588-594. ZHANG X N, NIE J Y, YAN Z, et al. Simultaneous detection of 7 kinds of mycotoxins in apple andits products by UPLC-MS/MS[J]. Journal of Instrumental Analysis, 2017, 36（5）: 588-594.
[14] 赵凯. 食品中交链格毒素污染、生物利用及风险评估硏究[D]. 北京: 中国疾控中心营养与健康所, 2015. ZHAO K. Naturaloccurence, bioavailability and risk assessment of Alternaria toxins in foods[D]. Beijing: Institute of Nutrition and Health, China Center for Disease Control and Prevention, 2015.
[15] NOSER J, SCHNEIDER P, ROTHER M, et al. Determination of six Alternaria toxins with UPLC-MS/MS and their occurrence in tomatoes and tomato products from the Swiss market[J]. Mycotoxin Research, 2011, 27: 265-271.
[16] 周贻兵, 李磊, 吴玉田, 等. QuEChERS净化—超高效液相色谱—串联质谱法测定番茄中5种链格孢霉毒素的含量[J]. 理化检验: 化学分册, 2019, 55（9）: 1 036-1 041. ZHOU Y B, LI L, WU Y T, et al. Determination of five Alternaria toxins in tomatoes by UHPLC-MS/MS with purification by QuEChERS[J]. Physical Testing and Chemical Analysis Part B: Chemical Analysis, 2019, 55（9）: 1 036-1 041.
[17] 陈蓓, 吉文亮, 朱峰, 等. 江苏省内小麦粉中4种交链孢毒素污染情况调查[J]. 中国公共卫生, 2018, 34（3）: 393-395. CHEN B, JI W L, ZHU F, et al. Contamination of four Alternaria toxins in wheat flour sampled in Jiangsu province[J]. Chinese Journal of Public Health, 2018, 34（3）: 393-395.
[18] ZHOU J, XU J J, CAI Z, et al. Simultaneous determination of five Alternaria toxins in cereals using QuEChERS-based methodology[J]. Journal of chromatography B, 2017, 1 068/1 069: 15-23.
[19] WALRAVENS J, MIKULA H, RYCHLIK M, et al. Development and validation of an ultra-high-performance liquidchromatography tandem mass spectrometric method for thesimultaneous determination of free and conjugated Alternaria toxins in cereal-based foodstuffs[J]. Journal of Chromatography A, 2014, 1 372: 91-101.
[20] 苏爽, 张二鹏, 韩月哲, 等. 玉米中4种交链孢霉毒素的测定方法研究[J]. 河南工业大学学报（自然科学版）, 2020, 41（3）: 72-77. SU S, ZHAGN E P, HAN Y Z, et al. Study on the determination methods of four Alternaria mycotoxins in maize[J]. Journal of Henan University and Technology （Natural Science Edition）, 2020, 41（3）: 72-77.
[21] 李珍妮, 曾文祥. 酶联免疫吸附法在食品检验中的应用研究[J]. 现代食品, 2020（22）: 190-191, 196. LI Z N, ZENG W X. Application of ELISA in food inspection[J]. Modern Food, 2020（22）: 190-191, 196.
[22] YOON B K, SUT T N, YOO K Y, et al. Lipid bilayer coatings for rapid enzyme-linked immunosorbent assay[J]. Applied Materials Today, 2021, 24: 101128.
[23] 韩绍印, 杨胜利, 马刚军, 等. 酶联免疫吸附试验检测食管癌高、低发区粮食中交链孢酚单甲醚的研究[J]. 河南医科大学, 1995, 30（3）: 219-222. HAN S Y, YANG S L, MA G J, et al. Study on the examination of AME in the grains in areas of high and low in cidenees of esophageal cancer with ELISA[J]. Zhengzhou University, 1995, 30（3）: 219-222.
[24] 钟红, 马良, 张宇昊, 等. 细交链格孢菌酮酸间接竞争酶联免疫检测方法及配套试剂盒的研制[J]. 分析科学学报, 2017, 33（2）: 195-200. ZHONG H, MA L, ZHANG Y H, et al. Study on indirect competitive enzyme immunoassay and rapid detection kit for tenuazonic acid[J]. Journal of Analytical Science, 2017, 33（2）: 195-200.
[25] WANG J Y, PENG T, ZHANG X Y, et al. A novelhapten and monoclonal antibody-based indirect competitive ELISA for simultaneous analysis of alternariol and alternariol monomethyl ether in wheat[J]. Food Control, 2018, 94: 65-70.
[26] 黄双庆, 何秋霞, 卿太辉. 薄层色谱法在保健食品检验应用中的常见问题及解决方法[J]. 食品安全质量检测学报, 2020, 11（23）: 8 609-8 614. HUANG S Q, HE Q X, QING T H. Common problems and solutions in the application of thin layer chromatography in the inspection of health food[J]. Journal of Food Safety & Quality, 2020, 11（23）: 8 609-8 614.
[27] MATYSIKL G, GIRYN H. Gradient thin-layer chromatography and densitometry determination ofalternaria mycotoxins[J]. Chromatographia, 1996, 42（9）: 555-558.
[28] HASAN H A. Alternaria mycotoxins in black rot lesion of tomato fruit: Conditions and regulation of their production[J]. Mycopathologia, 1995, 130（3）: 171-177.
[29] 王琦, 蔡瑞, 王周利, 等. 果蔬制品中链格孢霉毒素检测与控制研究进展[J]. 食品安全质量检测学报, 2020, 11（1）: 114-120. WANG Q, CAI R, WANG Z L, et al. Advances in detection and control of Alternaria toxin in fruits and vegetables[J]. Journal of Food Safety & Quality, 2020, 11（1）: 114-120.
[30] PAN Y C, LIU X, LIU J, et al. Chemiluminescence sensors based on molecularly imprinted polymers for the determination of organophosphorus in milk[J]. Journal of Dairy Science, 2022, 105（4）: 3 019-3 031.
[31] SUN Y L, GAO P, HAN R, et al. A target-triggered signal chemiluminescence sensor for prostate specific antigen detection based on hollow porous silica encapsulated luminol by aptamers[J]. Sensors & Actuators, B: Chemical, 2021, 333: 129543.
[32] ALBERTO R Y, RAHMA A, JAVIER L U, et al. Eu（III）-Templated molecularly imprinted polymer used as a luminescent sensor for the determination of tenuazonic acid mycotoxin in food samples[J]. Sensors and Actuators, B: Chemical, 2021, 329: 129256.
[33] 叶云锋, 李研东, 吴雨洋, 等. 化学发光免疫分析方法检测粮食谷物中赭曲霉毒素A残留[J]. 食品安全质量检测学报, 2017, 8（7）: 2 787-2 791. YE Y F, LI Y D, WU Y Y, et al. Determination of ochratoxin A residues in grains and cereals by chemiluminescence immunoassa[J]. Journal of Food Safety & Quality, 2017, 8（7）: 2 787-2 791.
[34] 蒋艳, 余姓鸿, 谢礼, 等. 化学发光免疫方法在食品安全检测中的应用及展望[J]. 食品安全质量检测学报, 2020, 11（20）: 7 603-7 609. JIANG Y, YU X H, XIE L, et al. Application and prospect of chemiluminescence immunoassay in food safety detection[J]. Journal of Food Safety & Quality, 2020, 11（20）: 7 603-7 609.
[35] LIU B J, SU X B, YU G, et al. An automated chemiluminescent immunoassay （CLIA） detects SARS-CoV-2 neutralizing antibody levels in COVID-19 patients and vaccinees[J]. International Journal of Infectious Diseases, 2022, 115: 116-125.
[36] YAO C Y, XU Z L, WANG H, et al. High affinity antibody based on a rationally designed hapten and development of a chemiluminescence enzyme immunoassay for quantification of alternariol in fruit Juice, maize and flour[J]. Food Chemistry, 2019, 283: 359-366.
[37] 马良, 钟红, 王佳曼, 等. 细交链格孢菌酮酸化学发光酶联免疫吸附分析方法[J]. 分析试试验室, 2017, 36（6）: 667-670. MA L, ZHONG H, WANG J M, et al. Indirect competitive chemiluminescence enzyme-linked immunoassay for tenuazonic acid[J]. Chinese Journal of Analysis Laboratory, 2017, 36（6）: 667-670.
[38] OUYANG H, XIAN J X; GAO J Q, et al. Highly sensitive chemiluminescent immunoassay of mycotoxins using ZIF-8-derived yolk-shell co single-atom site catalysts as superior fenton-like probes[J]. Analytical Chemistry, 2022, 94（7）: 3 400-3 407.
[39] 张文, 吕航, 金昱言. 食品分析中气相色谱—质谱联用技术应用概述[J]. 现代食品, 2021（1）: 110-112. ZHANG W, LU H, JIN Y Y. Overview of the application of gas chromatography-mass spectrometry technology in food analysis[J]. Modern Food, 2021（1）: 110-112.
[40] HARVAN D J, PERO R W. Gas chromatographic analysis of theAlternaria metabolite, tenuazonic acid[J]. Journal of Chromatography A, 1974, 101（1）: 222-224.
[41] SCOTT P M, WEBER D, KANHERE S R. Gas chromatography-mass spectrometry of Alternaria mycotoxins[J]. Journal of Chromatography A, 1997, 765（2）: 255-263.
[42] MYRESIOTIS C K, TESTEMPASIS S, VRYZAS Z, et al. Determination of mycotoxins in pomegranate fruits and juices using a QuEChERS-based method[J]. Food Chemistry, 2015, 182: 81-88.
[43] PAVON M , LUNA A, CRUZ S D L, et al. PCR-based assay for the detection of Alternaria species and orrelation with HPLC determination of altenuene, alternariol and alternariol monomethyl ether production in tomato products[J]. Food Control, 2012, 25（1）: 45-52.
[44] RICO-YUSTE A, GOMEZ-ARRIBAS L N, PEREZ-CONDE M C, et al. Rapid determination of Alternaria mycotoxins in tomato samples by pressurised liquid extraction coupled to liquid chromatography with fluorescence detection[J]. Food Additives and Contaminants: Part A, 2018, 35（11）: 2 175-2 182.
[45] 孙丹蕾. 新兴真菌毒素检测方法及膳食暴露研究[D]. 武汉: 武汉轻工大学, 2015: 6. SUN D L. Study on the methods for the determination of emerging mycotoxins and dietary exposure assessment in China[D]. Wuhan: Wuhan Polytechnic University, 2015: 6.
[46] ZHANG X M, QU J Y, DAI Z J, et al. Data-dependent acquisition based high-resolution mass spectrum for trace Alternaria mycotoxin analysis and sulfated metabolites identification[J]. Food Chemistry, 2021, 364: 130450.
[47] 袁航, 丁同英. 食品中主要真菌毒素检测方法研究进展[J]. 食品与机械, 2020, 36（12）: 203-206. YUAN H, DING T Y. Progress on detection methods of main mycotoxins in food[J]. Food & Machinery, 2020, 36（12）: 203-206.
[48] DE-BERARDISA S, DE PAOLA E L, MONTEVECCHIC G, et al. Determination of four Alternaria alternata mycotoxins by QuEChERS approach coupled with liquid chromatography-tandem mass spectrometry in tomato-based and fruit-based products[J]. Food Research International, 2018, 106: 677-685.
[49] WALRAVENS J, MILULA H, RYCHLIK M, et al. Validated UPLC-MS/MS methods to quantitate free and conjugated Alternaria toxins in commercially available tomato products and fruit and vegetable juices in Belgium[J]. Journal of Agriculture and Food Chemistry, 2016, 64（24）: 5 101-5 109.
[50] 陈跃, 张悦丽, 王超, 等. QuEChERS-高效液相色谱—串联质谱法测定水果中5种链格孢霉毒素[J]. 理化检验: 化学分册, 2019, 55（11）: 1 312-1 317. CHEN Y, ZAHGN Y L, WANG C, et al. HPLC-MS/MS determination of 5 Alternaria toxins in fruits with QuEChERS[J]. Physical Testing and Chemical Analysis Part B: Chemical Analysis, 2019, 55（11）: 1 312-1 317.
[51] RICO-YUSTE A, WALRAVENS J, URRACA J L, et al. Analysis ofalternariol and alternariol monomethyl ether in foodstuffs by molecularly imprinted solid-phase extraction and ultra-high-performance liquid chromatography tandem mass spectrometry[J]. Food Chemistry, 2018, 243: 357-364.
[52] 柴银皎, 李响敏, 熊勇华, 等. 分子印迹聚合物在真菌毒素检测中的应用[J]. 食品与发酵工业. 2018, 44（1）: 269-279. CHAI Y J, LI X M, XIONG Y H, et al. Application of molecularly imprinted polymers in mycotoxin determination[J]. Food and Fermentation Industries, 2018, 44（1）: 269-279.