Whole genome analysis and molecular traceability of Pseudomonas aeruginosa in drinking water

Authors

GUO Kun-peng, Hunan Institute of Commodity Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117, China
ZHANG Hai-yun, Hunan Institute of Commodity Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117, China
HE Juan, Hunan Institute of Commodity Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117, China
GAO Han, Hunan Institute of Commodity Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117, China
YANG Tao, Hunan Institute of Commodity Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117, China
SONG Sheng, Hunan Institute of Commodity Quality Inspection, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117, ChinaFollow

Corresponding Author(s)

宋晟（1979—），男，湖南省产商品质量检验研究院高级工程师，硕士。E-mail:83071480@qq.com

Abstract

Objective: Provide data support for the source tracing and pollution prevention of Pseudomonas aeruginosa in drinking water in Hunan Province. Methods: According to the national food safety standards of GB 8538—2016, the bottled water was tested randomly collected from partial cities and counties in Hunan Province, and the whole genome sequencing and molecular traceability of the 18 strains of Pseudomonas aeruginosa were detected. The carrying of virulence genes and drug resistance genes was detected. Results: It was confirmed that four strains of Pseudomonas aeruginosa showed consistency in terms of geographical distance, genetic distance, pathogenic and drug-resistant genotypes, and it was presumed that there was the same source. Conclusion: The traceability analysis of Pseudomonas aeruginosa in drinking water and the analysis of virulence and drug resistance genes in drinking water can be realized by whole gene sequencing based on 16S rRNA and single-copy orthologous genes.

Publication Date

10-20-2023

First Page

6

Last Page

12,61

DOI

10.13652/j.spjx.1003.5788.2022.60150

Recommended Citation

Kun-peng, GUO; Hai-yun, ZHANG; Juan, HE; Han, GAO; Tao, YANG; and Sheng, SONG (2023) "Whole genome analysis and molecular traceability of Pseudomonas aeruginosa in drinking water," Food and Machinery: Vol. 39: Iss. 7, Article 2.
DOI: 10.13652/j.spjx.1003.5788.2022.60150
Available at: https://www.ifoodmm.cn/journal/vol39/iss7/2

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