Optimization of complex enzymatic extraction process of steroidal glycosides from Cynanchum auriculatum Royle ex Wight and its hepatoprotective activity

Authors

SONG Wan-yu, College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China;Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
DAI Jing, College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China
FENG Jin, Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
LI Chun-yang, Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, ChinaFollow
MA Yong-qiang, College of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China

Corresponding Author(s)

李春阳（1966—），男，江苏省农业科学院农产品加工研究所研究员，博士。E-mail：lichunyang968@126.com

Abstract

Objective: Response surface methodology was used to optimize the extraction process of steroidal glycosides from Cynanchum auriculatum Royle ex Wight by complex enzymatic method, and its effects on alcohol dehydrogenase activity and human hepatoma cell HepG2 were analyzed. Methods: Four experimental factors; enzymolysis duration, temperature, compound enzyme dose and pH were used as responses to a single-factor experiment of the Response Surface Methodology on the steroidal glycoside yields, using perennial C. auriculatum as the target material. The alcohol-induced hepatocyte damage model of HepG2 was used to investigate the contents of ALT, LDH, and GSH and changes in cell shape. Results: The results show that the optimum extraction conditions of steroidal glycoside enzyme were obtained at an enzymolysis duration of 100 min, a temperature of 46 ℃, enzyme dose of 1.30% and pH of 5.0. Consequently, the actual extraction rate of （2.80±0.04）% was achieved using the optimized process conditions, which was close to the expected value. Compared with the model group, it significantly suppresses the growth of ALT and LDH while increasing the amount of intracellular GSH, and the cell damage was restored. Conclusion: The compound enzyme method can effectively extract the steroidal glycosides of Cynanchum auriculatum Royle ex Wight; steroidal glycosides of C. auriculatum have hepatoprotective effect.

Publication Date

10-20-2023

First Page

172

Last Page

179,201

DOI

10.13652/j.spjx.1003.5788.2023.60034

Recommended Citation

Wan-yu, SONG; Jing, DAI; Jin, FENG; Chun-yang, LI; and Yong-qiang, MA (2023) "Optimization of complex enzymatic extraction process of steroidal glycosides from Cynanchum auriculatum Royle ex Wight and its hepatoprotective activity," Food and Machinery: Vol. 39: Iss. 7, Article 26.
DOI: 10.13652/j.spjx.1003.5788.2023.60034
Available at: https://www.ifoodmm.cn/journal/vol39/iss7/26

References

[1] 印鑫, 丁永芳, 邵久针, 等. 白首乌的研究进展[J]. 中草药, 2019, 50（4）: 992-1 000. YIN X, DING Y F, SHAO J Z, et al. Research progress on Cynanchi Bungei Radix[J]. Chinese Traditional and Herbal Drugs, 2019, 50（4）: 992-1 000.
[2] WANG L, CAI F J, ZHAO W, et al. Cynanchum auriculatum Royle ex Wight., Cynanchum bungei Decne. and Cynanchum wilfordii （Maxim.） Hemsl.: Current Research and Prospects[J]. Molecules, 2021, 26（23）: 7 065-7 105.
[3] CHAI Z, HUANG W Y, ZHAO X, et al. Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of polysaccharide from Cynanchum auriculatum Royle ex Wight[J]. International Journal of Biological Macromolecules, 2018, 119: 1 068-1 076.
[4] UCHIKURA T, TANAKA H, SUGIWAKI H, et al. Preliminary quality evaluation and characterization of phenolic constituents in Cynanchi wilfordii Radix[J]. Molecules, 2018, 23（3）: 656-667.
[5] 庄子锐, 王明亮, 彭蕴茹, 等. 白首乌C₂₁甾苷通过TLR4通路防治大鼠肝肾纤维化的作用研究[J]. 中国中药杂志, 2021, 46（11）: 2 857-2 864. ZHUANG Z R, WANG M L, PENG Y R, et al. Effect of C₂₁ steroidal glycoside of Cynanchum auriculatum on liver and kidney fibrosis through TLR4 pathway[J]. China Journal of Chinese Materia Medica, 2021, 46（11）: 2 857-2 864.
[6] 张明, 沈明晨, 陈镭, 等. 白首乌的化学成分、药理作用及栽培技术综述[J]. 江苏农业科学, 2022, 50（2）: 22-29. ZHANG M, SHEN M C, CHEN L, et al. Chemical constituents, pharmacological effects and cultivation techniques of Cynanchum auriculatum[J]. Jiangsu Agricultural Sciences, 2022, 50（2）: 22-29.
[7] 陈其燕, 石云, 洪键. 滨海白首乌多糖的提取与药理活性研究进展[J]. 现代农业科技, 2020（19）: 215-216, 220. CHEN Q Y, SHI Y, HONG J. Research progress on extraction and pharmacological activity of Polysaccharide from Binhai's Cynanchum auriculatum[J]. Modern Agricultural Science and Technology, 2020（19）: 215-216, 220.
[8] 许慧卿. 滨海白首乌甾苷提取工艺优化及其抗氧化活性研究[D]. 南京: 南京农业大学, 2019: 2-24. XU H Q. Optimization on the extraction of steroidal glycosides from Binhai's Cynanchum auriculatum Royle ex Wight and its antioxidant activity[D]. Nanjing: Nanjing Agricultural University, 2019: 2-24.
[9] 田鹭. 超临界CO₂萃取白首乌中C₂₁甾苷的工艺研究[D]. 镇江: 江苏大学, 2009: 33-56. TIAN L. Extraction of C₂₁ steroidal glycosides from Cynanchum auriculatum Royle ex wight with supercritical carbon dioxide[D]. Zhenjiang: Jiangsu University, 2009: 33-56.
[10] 李文君, 王成章, 张水晶, 等. 多元复合酶降解黄姜薯蓣皂苷元的工艺研究[J]. 生物质化学工程, 2014, 48（4）: 23-27. LI W J, WANG C Z, ZHANG S J, et al. Study on the degradation of Diosgenin by multiplex enzyme[J]. Biomass Chemical Engineering, 2014, 48（4）: 23-27.
[11] WU Z H, WANG Y Y, MENG X, et al. Total C-21 steroidal glycosides, isolated from the root tuber of Cynanchum auriculatum Royle ex Wight, attenuate hydrogen peroxide-induced oxidative injury and inflammation in L02 cells[J]. International Journal of Molecular Medicine, 2018, 42（6）: 3 157-3 170.
[12] 梁诗, 李煊, 陈良华, 等. 野菊水提物和醇提物抗氧化及保肝活性研究[J]. 亚热带植物科学, 2019, 48（4）: 329-333. LIANG S, LI X, CHEN L H, et al. Study on antioxidant and hepatoprotective activities of water extracts and alcohol extracts of wild chrysanthemum[J]. Subtropical Plant Science, 2019, 48（4）: 329-333.
[13] LIU H, LI J Y, YUAN W W, et al. Bioactive components and mechanisms of poplar propolis in inhibiting proliferation of human hepatocellular carcinoma HepG2 cells[J]. Biomedicine & Pharmacotherapy, 2021, 144: 112364.
[14] PENG Y L, HU K. Effect of garlic on rats with chronic intermittent hypoxia combined with diabetes mellitus[J]. Molecular Medicine Reports, 2018, 17（4）: 6 174-6 184.
[15] 饶智, 陈光宇, 谢梦洲, 等. 芦根提取物对大鼠急性酒精性肝损伤的保护作用研究[J]. 时珍国医国药, 2022, 33（1）: 95-98. RAO Z, CHEN G Y, XIE M Z, et al. Study on the protective effect of Reed root extract on acute alcoholic liver injury in rats[J]. Shi Zhen Chinese Medicine, 2022, 33（1）: 95-98.
[16] MUSTAFA K, YU S, ZHANG W, et al. Screening, characterization, and in vitro-ROS dependent cytotoxic potential of extract from Ficus carica against hepatocellular （HepG2） carcinoma cells[J]. South African Journal of Botany, 2021, 138: 217-226.
[17] 刘甜甜, 杨舒涵, 颜梅, 等. 柠檬提取物和水飞蓟素对大鼠急性酒精性肝损伤的保护作用研究[J]. 西南医科大学学报, 2021, 44（2）: 105-109. LIU T T, YANG S H, YAN M, et al. Protective effect of lemon extract and silymarin on acute alcoholic liver injury in rats[J]. Journal of Southwest Medical University, 2021, 44（2）: 105-109.
[18] WANG Y S, ZHANG N H, ZHOU J X, et al. Protective effects of several common amino acids, vitamins, organic acids, flavonoids and phenolic acids against hepatocyte damage caused by alcohol[J]. Foods, 2022, 11（19）: 3 014.