Abstract
Objective: This study aimed to reduce inflammation effect caused by long-term and large amount of betel nut chewing. Methods: Natural plant extracts with anti-inflammatory effects were added to betel nut in order to reduce the inflammatory damage. Glycyrrhiza uralensis fisch, radix platycodi and loquat leaf were used to treat rats with areca nut for 28 days. Body weight, organ coefficient, swelling rate of plantar in 6 hours, levels of inflammatory factors, and mediators were compared. Results: The addition of high-dose natural plant extract significantly reduced the production of prostaglandin E2 (PGE2), malondialdehyde (MDA), and nitric oxide (NO) compared to betel nut used group. Less pro-inflammatory tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and more release of interleukin-10 (IL-10) were observed. It also reduced the production of reactive oxygen species (ROS) in the liver. The swelling rate of rats' feet (P＜0.05) was significantly lower than the control group, and similar to the positive control group. Conclusion: The addition of natural plant extracts with anti-inflammatory effect can reduce the acute inflammation in rats, and provide a new development idea for the further processing of areca catechu.
Publication Date
10-20-2023
First Page
142
Last Page
146,226
DOI
10.13652/j.spjx.1003.5788.2023.80050
Recommended Citation
Xiaohui, SONG; Junding, ZHU; Zhi, LI; Chaoyang, MA; and Hongxin, WANG
(2023)
"Effects of plant extract and betel nut on acute inflammation in rats,"
Food and Machinery: Vol. 39:
Iss.
8, Article 22.
DOI: 10.13652/j.spjx.1003.5788.2023.80050
Available at:
https://www.ifoodmm.cn/journal/vol39/iss8/22
References
[1] GUPTA P C, RAY C S. Epidemiology of betel quid usage[J]. Annals of the Academy of Medicine Singapore, 2004, 33: 31-36.
[2] CHEN P H, TSAI C C, LIN Y C, et al. Ingredients contribute to variation in production of reactive oxygen species by areca quid[J]. Journal of Toxicology and Environmental Health, Part A, 2006, 69（11）: 1 055-1 069.
[3] JI W T, LEE C I, CHEN J Y F, et al. Areca nut extract induces pyknotic necrosis in serum-starved oral cells via increasing reactive oxygen species and inhibiting GSK3β: An implication for cytopathic effects in betel quid chewers[J]. PLoS One, 2013, 8（5）: e63295.
[4] 栾剑, 郭迪, 周晓馥. 槟榔致癌性和毒性的药理学研究进展[J]. 食品与机械, 2019, 35（2）: 185-189, 236.
LUAN J, GUO D, ZHOU X F. Progress in pharmacological research on carcinogenicity and toxicity of betel nut[J]. Food & Machinery, 2019, 35（2）: 185-189, 236.
[5] LIU S Y, FENG I J, WU Y W, et al. Implication for second primary cancer from visible oral and oropharyngeal premalignant lesions in betel-nut chewing related oral cancer[J]. Head & Neck, 2017, 39（7）: 1 428-1 435.
[6] 肖旭坤, 王翰华, 阮洪生. 枇杷叶化学成分和药理活性研究进展[J]. 中医药导报, 2019, 25（21）: 60-66.
XIAO X K, WANG H H, RUAN H S. Research progress on chemical composition and pharmacological activity of loquat leaf[J]. Herald of Traditional Chinese Medicine, 2019, 25（21）: 60-66.
[7] DOS S M D, ALMEIDA M C, LOPES N P, et al. Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid[J]. Biologicaland Pharmaceutical Bulletin, 2006, 29（11）: 2 236-2 240.
[8] GONTHIER M P, VERNY M A, BESSON C, et al. Chlorogenic acid bioavailability largely depends on its metabolism by the gut microflora in rats[J]. The Journal of Nutrition, 2003, 133（6）: 1 853-1 859.
[9] SHAN J H, FU J, ZHAO Z H, et al. Chlorogenic acid inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in RAW264.7 cells through suppressing NF-κB and JNK/AP-1 activation[J]. International Immunopharmacology, 2009, 9（9）: 1 042-1 048.
[10] ASSAR D H, ELHABASHI N, MOKHBATLY A A, et al. Wound healing potential of licorice extract in rat model: Antioxidants, histopathological, immunohistochemical and gene expression evidences[J]. Biomedicine & Pharmacotherapy, 2021, 143: 112151.
[11] AYEKA P A, BIAN Y, MWITARI P G, et al. Immunomodulatory and anticancer potential of Gan cao （Glycyrrhiza uralensis Fisch.） polysaccharides by CT-26 colon carcinoma cell growth inhibition and cytokine IL-7 upregulation in vitro[J]. BMC Complementary and Alternative Medicine, 2016, 16（1）: 1-8.
[12] AYEKA P A, BIAN Y, GITHAIGA P M, et al. The immunomodulatory activities of licorice polysaccharides （Glycyrrhiza uralensis Fisch.） in CT 26 tumor-bearing mice[J]. BMC Complementary and Alternative Medicine, 2017, 17（1）: 1-9.
[13] 李泽宇, 郝二伟, 李卉, 等. 甘草配伍应用的药理作用及机制分析[J]. 中国实验方剂学杂志, 2022, 28（14）: 270-282.
LI Z Y, HAO E W, LI H, et al. Pharmacological action and mechanism analysis of licorice in combination[J]. Chinese Journal of Experimental Medical Formulae, 2022, 28（14）: 270-282.
[14] 王萌, 韩嘉祺, 张凤, 等. 桔梗皂苷D对小鼠脾淋巴细胞免疫调节活性的研究[J]. 中国兽医科学, 2018, 48（1）: 93-100.
WANG M, HAN J Q, ZHANG F, et al. Study on immunomodulatory activity of bellflower saponin D on splenic lymphocytes in mice[J]. China Veterinary Science, 2018, 48（1）: 93-100.
[15] 范文京, 任娟宁, 战秀俊, 等. 桔梗汤及其活性成分治疗急性肺损伤的作用机制研究进展[J]. 中草药, 2022, 53（4）: 1 230-1 239.
FAN W J, REN J N, ZHAN X J, et al. Research progress on the mechanism of action of campanula soup and its active ingredients in the treatment of acute lung injury[J]. Chinese Traditional and Herbal Drugs, 2022, 53（4）: 1 230-1 239.
[16] 杨慧, 戴铭睿, 于洋, 等. 基于转录组数据分析地下水污染对ICR小鼠肝脏遗传损伤的分子机制[J]. 动物医学进展, 2022, 43（10）: 44-49.
YANG H, DAI M R, YU Y, et al. Molecular mechanism of groundwater pollution on liver genetic damage in ICR mice based on transcriptome data analysis[J]. Progress in Veterinary Medicine, 2022, 43（10）: 44-49.
[17] 李晓萌, 钟伟, 潘香香, 等. 两种川贝复方制剂抗炎作用及其机制研究[J]. 现代预防医学, 2018, 45（7）: 1 288-1 291, 1 300.
LI X M, ZHONG W, PAN X X, et al. Anti-inflammatory effect and mechanism of twoChuanbei compound preparations[J]. Modern Prevent Medicine, 2018, 45（7）: 1 288-1 291, 1 300.
[18] 阮洪生, 刘树民. 身痛逐瘀胶囊对醋酸致痛小鼠血清中NO、SOD、MDA、PGE2的影响[J]. 中药药理与临床, 2013, 29（5）: 6-8.
RUAN H S, LIU S M. Effects of body pain capsule on NO, SOD, MDA, PGE2 in serum ofmice with acetic acid pain[J]. Journal of Traditional Chinese Materia Medica, 2013, 29（5）: 6-8.
[19] 李恒聪, 陈蔚, 于清泉, 等. 感觉/交感神经参与结肠炎模型大鼠敏化区域皮肤温度、血流灌注和皮肤组织炎性细胞因子变化[J]. 中国针灸, 2022, 42（7）: 785-793.
LI H C, CHEN W, YU Q Q, et al. Changes in skin temperature, blood perfusion and inflammatory cytokines in skin tissue in rats with sensory/sympathetic involvement in colitis model rats[J]. Chinese Journal of Acupuncture, 2022, 42（7）: 785-793.
[20] 严皓哲, 汤璧嘉, 王勇兴. 复方猴头颗粒联合西药治疗老年胃溃疡临床疗效及对血清学指标的影响[J]. 新中医, 2022, 54（7）: 89-92.
YAN H Z, TANG B J, WANG Y X. Clinical efficacy of compound monkey head granules combined with western medicine in the treatment of gastric ulcer in elderly elderly people and its effect on serological indexes[J]. New Chinese Medicine, 2022, 54（7）: 89-92.
[21] 任东根, 龚婷, 王丽娟, 等. 川麦冬多糖的结构特征及对过度运动引起小鼠肝损伤的改善作用[J/OL]. 食品与发酵工业. （2022-11-27）[2023-04-28]. https://doi.org/10.13995/j.cnki.11-1802/ts.033543.
REN D G, GONG T, WANG L J, et al. Structural characteristics of polysaccharides and their effect on liver injury caused by excessive exercise[J/OL]. Food and Fermentation Industry. （2022-11-27）[2023-04-28]. https://doi.org/10.13995/j.cnki.11-1802/ts.033543.