Screening of autochthonouslactic acid bacteria and optimization of fermentation process in low-salt Zhayu of red rice

Authors

XU Dongmei, College of Food and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China;Hunan Provincial Engineering Technology Research Center of Aquatic Food Resources Processing, Changsha, Hunan 410114, China
LIU Yongle, College of Food and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China;Hunan Provincial Engineering Technology Research Center of Aquatic Food Resources Processing, Changsha, Hunan 410114, China
LI Xianghong, College of Food and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China;Hunan Provincial Engineering Technology Research Center of Aquatic Food Resources Processing, Changsha, Hunan 410114, China
WANG Faxiang, College of Food and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China;Hunan Provincial Engineering Technology Research Center of Aquatic Food Resources Processing, Changsha, Hunan 410114, China
HUANG Yiqun, College of Food and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China;Hunan Provincial Engineering Technology Research Center of Aquatic Food Resources Processing, Changsha, Hunan 410114, China
MA Xiayin, College of Food and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China;Hunan Provincial Engineering Technology Research Center of Aquatic Food Resources Processing, Changsha, Hunan 410114, ChinaFollow

Corresponding Author(s)

马夏吟（1991—），女，长沙理工大学讲师，博士。E-mail：maxiayin@csust.edu.cn

Abstract

Objective: To solve the problems of an uncertain fermentation process, a long fermentation period, and the unstable quality of traditional naturally fermented preserved fish. Methods: The lactic acid bacteria were isolated from low-salt preserved red fish, and the inhibition ability, salt tolerance, and acid production ability of the strains were investigated. The lactic acid bacteria suitable for fermentation were screened out, and the fermentation of preserved red fish was intensified by inoculation. The amount of red rice flour added, the amount of salt added during curing, and the fermentation temperature were optimized in the fermentation process. Results: There were 22 strains of Pediococcus with inhibitory effect on Escherichia coli or Staphylococcus aureus were isolated, among which Pediococcus pentosaceus P-56 had the best inhibitory effect on Escherichia coli and higher tolerance to bile salt and acid; After inoculation fermentation and process optimization. The pH value of the Zhayu decreased to 4.42 at 4 days of fermentation, the TCA-soluble peptide content was 434.99 μmol/g and the sensory score reached 45.7. Conclusion: The optimal process conditions for the fermentation of preserved red fish with lactic acid bacteria were: 4% salt addition for curing, 4% red yeast rice flour addition and fermentation temperature of 32 ℃.

Publication Date

10-20-2023

First Page

175

Last Page

185

DOI

10.13652/j.spjx.1003.5788.2023.60040

Recommended Citation

Dongmei, XU; Yongle, LIU; Xianghong, LI; Faxiang, WANG; Yiqun, HUANG; and Xiayin, MA (2023) "Screening of autochthonouslactic acid bacteria and optimization of fermentation process in low-salt Zhayu of red rice," Food and Machinery: Vol. 39: Iss. 8, Article 27.
DOI: 10.13652/j.spjx.1003.5788.2023.60040
Available at: https://www.ifoodmm.cn/journal/vol39/iss8/27

References

[1] 康慎敏, 徐睿, 武瑞赟. 乳酸菌在发酵鱼制品中的应用[J]. 中国水产, 2021（7）: 84-87. KANG S M, XU R, WU R Z. Application of lactic acid bacteria in fermented fish products[J]. China Fisheries, 2021（7）: 84-87.
[2] 于美娟, 谭欢, 马美湖, 等. 腌制工艺对固态发酵鲊鱼品质的影响[J]. 湖南农业科学, 2017（3）: 90-93. YU M J, TAN H, MA M H, et al. Effect of curing process on the quality of solid fermented Zhayu[J]. Hunan Agricultural Science, 2017（3）: 90-93.
[3] MELINI F, MELINI V, LUZIATELLI F, et al. Health-promoting components in fermented foods: An up-to-date systematic review[J]. Nutrients, 2019, 11（5）: 1 189.
[4] ANIL K A. Quality ingredients and safety concerns for traditional fermented foods and beverages from Asia: A review[J]. Fermentation, 2019, 5（1）: 5010008.
[5] 林城杏. 传统高盐发酵酸鱼乳酸菌菌群结构及强化发酵作用研究[D]. 贵阳: 贵州大学, 2019: 3-10. LIN C X. Research on the structure of lactic acid bacteria flora and strengthening fermentation effect of traditional high-salt fermented sour fish[D]. Guiyang: Guizhou University, 2019: 3-10.
[6] LEE S H, JUNG J Y, JEON C O. Microbial successions and metabolite changes during fermentation of salted shrimp （saeu-jeot） with different salt concentrations[J]. PLoS One, 2014, 9（2）: e90115.
[7] INGUGLIA E S, ZHANG Z H, TIWARI B K, et al. Salt reduction strategies in processed meat products: A review[J]. Trends in Food Science & Technology, 2017, 59: 70-78.
[8] 聂黔丽, 王修俊, 周雯, 等. 低钠复合盐腌制对苗岭腊肉品质的影响[J]. 包装工程, 2021, 42（19）: 114-121. NIE Q L, WANG X J, ZHOU W, et al. Effect of low sodium compound salt curing on the quality of Miaoling cured meat[J]. Packaging Engineering, 2021, 42（19）: 114-121.
[9] XU Y S, LI L, REGENSTEIN J M, et al. The contribution of autochthonous microflora on free fatty acids release and flavor development in low-salt fermented fish[J]. Food Chemistry, 2018, 256: 259-267.
[10] 田旭艳. 低盐发酵鳊鱼品质提升工艺及冻藏稳定性研究[D]. 无锡: 江南大学, 2022: 3-7. TIAN X Y. Study on the quality enhancement process and freezing stability of low-salt fermented bream[D]. Wuxi:Jiangnan University, 2022: 3-7.
[11] BROWN I J, TZOULAKI I, CANDEIAS V, et al. Salt intakes around the world: Implications for public health[J]. International Journal of Epidemiology, 2009, 38（3）: 791-813.
[12] 何丽娜. 发酵工艺对鲤鱼质构及食用品质的影响[D]. 无锡: 江南大学, 2019: 5-24. HE L N. Effect of fermentation process on the texture and eating quality of carp[D]. Wuxi: Jiangnan University, 2019: 5-24.
[13] 雷跃磊, 卢素芳, 张光华, 等. 湖北风味发酵鳜鱼加工关键工艺[J]. 食品工业科技, 2018, 39（16）: 155-160. LEI Y L, LU S F, ZHANG G H, et al. Key process of processing fermented Mandarin fish with Hubei flavor[J]. Food Industry Science and Technology, 2018, 39（16）: 155-160.
[14] VENMATHI E, NIRUPAMA A. Impact of perceived self-efficacy among hypertensives in adapting to low salt diet[J]. Current Medical Issues, 2022, 20（2）: 57-62.
[15] YANG G H, ZHOU X, JI W J, et al. Effects of a low salt diet on isolated systolic hypertension: A community-based population study[J]. Medicine, 2018, 97（14）: e0342.
[16] 谭汝成, 欧阳加敏, 卢晓莉, 等. 接种植物乳杆菌和戊糖片球菌发酵对鱼鲊品质的影响[J]. 食品科学, 2007（12）: 268-272. TAN N C, OUYANG J M, LU X L, et al. Effect of fermentation with Lactobacillus plantarum and Pediococcus pentosaceus on the quality of fermented fish products[J]. Food Science, 2007（12）: 268-272.
[17] WANG S, YUAN X, DONG Z, et al. Characteristics of isolated Lactic acid bacteria and their effects on the silage quality[J]. Asian-Australasian Journal of Animal Sciences, 2017, 30（6）: 819-827.
[18] 朱雯娟, 安俊莹, 张雪梅, 等. 梅香鱼发酵菌株的筛选及对品质的影响[J]. 食品科学, 2015, 36（23）: 162-166. ZHU W J, AN J Y, ZHANG X M, et al. Screening of fermentative strains of plum-flavored fish and their effects on quality[J]. Food Science, 2015, 36（23）: 162-166.
[19] AN Y Q, CAI X W, CONG L, et al. Quality improvement of Zhayu, a fermented fish product in China: Effects of inoculated fermentation with three kinds of lactic acid bacteria[J]. Foods, 2022, 11（18）: 2 756-2 756.
[20] XU Y S, ZANG J H, REGENSTEIN J M, et al. Technological roles of microorganisms in fish fermentation: A review[J]. Critical Reviews in Food Science and Nutrition, 2021, 61（6）: 1 000-1 012.
[21] HAN J, ZHANG J B, LIN X P, et al. Effect of autochthonous lactic acid bacteria on fermented Yucha quality[J]. LWT, 2020, 123（C）: 109060.
[22] GAO P, WANG W X, JIANG Q X, et al. Effect of autochthonous starter cultures on the volatile flavour compounds of Chinese traditional fermented fish （Suan yu）[J]. International Journal of Food Science & Technology, 2016, 51（7）: 1 630-1 637.
[23] 孙熙浛, 崔承弼, 齐仕博, 等. 低盐泡菜中耐酸性乳酸菌的筛选、鉴定及特性研究[J]. 食品与机械, 2022, 38（1）: 24-31, 37. SUN X H, CUI C M, QI S B, et al. Screening, identification and characteristics of acid-tolerant lactic acid bacteria in low-salt pickles[J]. Food & Machinery, 2022, 38（1）: 24-31, 37.
[24] SPERANZA B, RACIOPPO A, BENEDUCE L, et al. Autochthonous lactic acid bacteria with probiotic aptitudes as starter cultures for fish-based products[J]. Food Microbiology, 2017, 65: 244-253.
[25] ZANG J H, XU Y S, XIA W S, et al. Correlations between microbiota succession and flavor formation during fermentation of Chinese low-salt fermented common carp （Cyprinus carpio L.） inoculated with mixed starter cultures[J]. Food Microbiology, 2020, 90: 103487.
[26] 张潇. 鲊鱼工业化生产关键技术研究[D]. 长沙: 中南林业科技大学, 2018: 2-4. ZHANG X. Research on key technologies for industrial production of preserved fish[D]. Changsha: Zhongnan University of Forestry Science and Technology, 2018: 2-4.
[27] 卢晓莉. 鱼鲊制品中乳酸菌的分离、筛选及应用[D]. 武汉: 华中农业大学, 2007: 6-39. LU X L. Isolation, screening and application of lactic acid bacteria from fermented fish products[D]. Wuhan: Huazhong Agricultural University, 2007: 6-39.
[28] 龚吉军, 唐静, 李忠海, 等. 响应曲面法优化鲊鱼发酵条件[J]. 中国食品学报, 2010, 10（4）: 219-226. GONG J J, TANG J, LI Z H, et al. Optimization of fermentation conditions of preserved fish by response surface method[J]. Chinese Journal of Food Science, 2010, 10（4）: 219-226.
[29] JIRASATID S, NOPHARATANA M, KITSUBUN P, et al. Degradation kinetics of monacolin K in red yeast rice powder using multiresponse modeling approach[J]. Journal of Food Engineering, 2013, 116（2）: 436-443.
[30] 李剑, 高向阳, 黄国平, 等. 红曲米粉在饼干中的着色研究[J]. 中国食品添剂, 2023, 34（3）: 205-220. LI J, GAO X Y, HUANG G P, et al. Study on the coloring of red yeast rice powder in biscuits[J]. China Food Additives, 2023, 34（3）: 205-220.
[31] VENDRUSCOLO F, BHLER R M M, DE CARVALHO J C, et al. Monascus: A reality on the production and application of microbial pigments[J]. Applied Biochemistry and Biotechnology, 2016, 178（2）: 211-223.
[32] KALAIVANI M, SABITHA R, KALAISELVAN V, et al. Health Benefits and clinical impact of major nutrient, red yeast rice: A review[J]. Food and Bioprocess Technology, 2010, 3（3）: 333-339.
[33] 王玉芬, 张建国. 红曲色素在肉制品中的应用[J]. 肉类工业, 2002（12）: 4-7. WANG Y F, ZHANG J G. Application of red yeast pigment in meat products[J]. Meat Industry, 2002（12）: 4-7.
[34] 童群义. 红曲霉产生的生理活性物质研究进展[J]. 食品科学, 2003（1）: 163-167. TONG Q Y. Progress in the study of physiologically active substances produced by Aspergillus oryzae[J]. Food Science, 2003（1）: 163-167.
[35] KIM C S, KIM H Y. Physicochemical properties of emulsion-type sausage added red yeast rice powder[J]. Korean Journal of Food Science and Technology, 2017, 49（4）: 396-400.
[36] IJHUI C, HYUK K J, KI K B, et al. Physicochemical properties of chicken thigh meat sausage manufactured with red yeast rice powder[J]. Korean Journal of Poultry Science, 2019, 46（1）: 11-15.
[37] 葛磊, 乔峰, 陈育红, 等. 红曲藜麦高蛋白饼干的研制[J]. 粮食与油脂, 2019, 32（10）: 42-45. GE L, QIAO F, CHEN Y H, et al. Development of red quinoa high-protein biscuits[J]. Grain and Fats, 2019, 32（10）: 42-45.
[38] 陈晶晶, 李佳根, 李兆琦, 等. 红曲米粉对香卤鱿鱼中晚期糖基终产物的影响[J]. 肉类工业, 2022（9）: 19-23. CHEN J J, LI J G, LI Z Q, et al. Effect of red yeast rice flour on the mid-and late-stage glycosylated end products of aromatic marinated squid[J]. Meat Industry, 2022（9）: 19-23.
[39] 吴康. 红曲菌发酵半干鲩鱼研制及其风味分析[D]. 广州: 暨南大学, 2020: 3-9. WU K. Development and flavor analysis of red yeast fermented grass carp[D]. Guangzhou: Jinan University, 2020: 3-9.
[40] 王乃富, 李春阳, 阎征, 等. 红曲霉发酵对鳙鱼肉糜品质和生物活性影响研究[J]. 广西农学报, 2010, 25（2）: 54-57. WANG N F, LI C Y, YAN Z, et al. Study on the effect of red Aspergillus fermentation on the quality and biological activity of bighead carp minced meat[J]. Guangxi Journal of Agriculture, 2010, 25（2）: 54-57.
[41] 佘之蕴, 黄宝莹, 刘海卿, 等. 牛津杯法测定食品添加剂对五种益生菌的抑菌活力[J]. 食品工业, 2016, 37（1）: 171-174. YU Z Y, HUANG B Y, LIU H Q, et al. Determination of the antibacterial activity of food additives against five probiotic bacteria by Oxford cup method[J]. Food Industry, 2016, 37（1）: 171-174.
[42] PHETSANG H, PANPIPAT W, UNDELAND I, et al. Comparative quality and volatilomic characterisation of unwashed mince, surimi, and pH-shift-processed protein isolates from farm-raised hybrid catfish （Clarias macrocephalus×Clarias gariepinus） [J]. Food Chemistry, 2021, 364: 130365.
[43] YANG J, LU J, ZHU Q Z, et al. Isolation and characterization of a novel Lactobacillus plantarum MMB-07 from traditional Suanyu for Acanthogobius hasta fermentation[J]. Journal of Bioscience and Bioengineering, 2021, 132（2）: 161-166.
[44] 曾雪峰, 夏文水. 湘西传统酸鱼中乳酸菌的分离及特性研究[J]. 食品与发酵工业, 2012, 38（12）: 40-44. ZENG X F, XIA W S. Isolation and characterization of lactic acid bacteria from traditional sour fish in Xiangxi[J]. Food and Fermentation Industry, 2012, 38（12）: 40-44.
[45] 王华娟. 酸鱼中乳酸菌的分离鉴定及其可控发酵工艺研究[D]. 武汉: 武汉轻工大学, 2016: 3-36. WANG H J. Isolation and identification of lactic acid bacteria from sour fish and its controlled fermentation process[D]. Wuhan: Wuhan Light Industry University, 2016: 3-36.
[46] 王艳婷. 戊糖片球菌C-2-1产细菌素的研究[D]. 上海: 上海海洋大学, 2016: 3-12. WANG Y T. Study on the production of bacteriocins by Pentachybotrys spp. C-2-1[D]. Shanghai: Shanghai Ocean University, 2016: 3-12.
[47] WANG W X, XIA W S, GAO P, et al. Proteolysis during fermentation of Suanyu as a traditional fermented fish product of China[J]. International Journal of Food Properties, 2017, 20: S166-S176.
[48] ZENG X F, XIA W S, JIANG Q X, et al. Effect of autochthonous starter cultures on microbiological and physico-chemical characteristics of Suanyu, a traditional Chinese low salt fermented fish[J]. Food Control, 2013, 33（2）: 344-351.