•  
  •  
 

Corresponding Author(s)

李镁娟(1983—),女,河南国德标检测技术有限公司高级工程师,硕士。E-mail:745045911@qq.com

Abstract

Objective: Develop the industrial production of Lentinus edodes stalk polysaccharides. Methods: Using the yield of polysaccharides as an indicator, uniform design experiments were used to optimize the extraction process parameters. Four methods were used to determine the antioxidant activity of polysaccharides and compared with the traditional hot water extraction method. Results: The optimal process conditions for the flash-assisted extraction of Lentinus edodes stalk polysaccharides were as follows: flash extraction time 120 s, ratio of liquid to material 40∶1 (mL/g), hot water extraction time 105min, temperature 50 ℃, extraction twice. Under these conditions, the yield of polysaccharides was (5.03±0.22)%, which was basically consistent with the predicted value of the model and 1.82 times higher than that of traditional hot water extraction method. Lentinus edodes stalk polysaccharides had strong Fe3+ reduction ability, total antioxidant capacity, ·OH and DPPH· scavenging capacity, and showed a dose-effect relationship. The antioxidant activity of polysaccharides obtained by flash-assisted hot water extraction was stronger than that of the traditional hot water extraction, but weaker than that of vitamin C. Conclusion: Flash-assisted hot water extraction is beneficial to the extraction of polysaccharides from Lentinus edodes stalks, and can maintain its antioxidant activity.

Publication Date

10-20-2023

First Page

186

Last Page

191

DOI

10.13652/j.spjx.1003.5788.2023.60038

References

[1] 中国食用菌协会. 2020年度全国食用菌统计调查结果分析[J]. 中国食用菌, 2022, 41(1): 85-91. China Edible Fungi Association. Analysis of the 2020 national statistical survey on edible fungi[J]. Edible Fungi of China, 2022, 41(1): 85-91.
[2] 孙宇晨, 部建雯, 张明, 等. 香菇柄功能成分协同降血脂活性研究[J]. 食品科技, 2022, 47(6): 112-119. SUN Y C, BU J W, ZHANG M, et al. Synergistic hypolipidemic activity of functional components from Lentinula edodes stem[J]. Food Science and Technology, 2022, 47(6): 112-119.
[3] LI S F, WANG A J, LIU L N, et al. Extraction of polysaccharides under vacuum condition from Lentinus edodes stipe and their antioxidant activities in vitro[J]. Food Science and Biotechnology, 2019, 28(3): 759-767.
[4] MAITY P, SEN I K, CHAKRABORTY I, et al. Biologically active polysaccharide from edible mushrooms: A review[J]. International Journal of Biological Macromolecules, 2021, 172: 408-417.
[5] 陕西师范大学. 一种基于香菇柄中性多糖的果汁抑菌剂: CN112753927B[P]. 2022-08-30. Shaanxi Normal University. A fruit juice antibacterial agent based on neutral polysaccharide from Lentinus edodes stalk: CN112753927B[P]. 2022-08-30.
[6] 张欣, 韩增华, 孔祥辉, 等. 酶法提取香菇柄多糖[J]. 生物技术, 1999, 9(1): 21-24. ZHANG X, HAN Z H, KONG X H, et al. Enzymatic extraction of polysaccharides from Lentinus edodes stalk[J]. Biotechnology, 1999, 9(1): 21-24.
[7] 王谦, 徐彩芳, 王朝江. 微波法提取香菇柄多糖初步研究[J]. 食用菌学报, 2009, 16(4): 58-60. WANG Q, XU C F, WANG C J. Optimization of polysaccharide extraction from Lentinus edodes stipes using microwave technology[J]. Acta Edulis Fungi, 2009, 16(4): 58-60.
[8] 朱晓梅, 田光辉. 香菇柄中多糖提取工艺的优选[J]. 食品与发酵工业, 2004, 30(3): 125-127. ZHU X M, TIAN G H. Optimizing extraction process of polysaccharide from root of Lentinus edodes[J]. Food and Fermentation Industries, 2004, 30(3): 125-127.
[9] 王琼波. 香菇菌柄与菌伞多糖含量及抗氧化性比较[J]. 饮料工业, 2017, 20(2): 16-19. WANG Q B. Comparative studies on the content of polysaccharide and its antioxidant activity in stipes and caps of Lentinus edodes[J]. Beverage Industry, 2017, 20(2): 16-19.
[10] 秦楠, 缪文玉, 连文绮, 等. 响应曲面法优化微波辅助提取香菇柄多糖及其抗氧化活性研究[J]. 食品安全质量检测学报, 2017, 8(2): 594-600. QIN N, MIAO W Y, LIAN W Q, et al. Optimization of microwave-assisted extraction of polysaccharides from Lentinus edods root by response surface analysis and its antioxidant activity[J]. Journal of Food Safety and Quality, 2017, 8(2): 594-600.
[11] 刘延泽. 植物组织破碎提取法及闪式提取器的创制与实践[J]. 中国天然药物, 2007, 5(6): 401-407. LIU Y Z. Principle and practice of smashing tissue extraction and herbal blitzkrieg extractor[J]. Chinese Journal of Natural Medicines, 2007, 5(6): 401-407.
[12] QIN D Y, XI J. Flash extraction: An ultra-rapid technique for acquiring bioactive compounds from plant materials[J]. Trends in Food Science & Technology, 2021, 112: 581-591.
[13] 张雄, 肖志勇, 黄群, 等. 猴头菇多糖和蛋白质闪式联合提取工艺优化及结构鉴定[J]. 食品与机械, 2019, 35(10): 117-121, 183. ZHANG X, XIAO Z Y, HUANG Q, et al. Optimization of flash extraction process and structure identification of polysaccharide and protein from Hericium erinaceus[J]. Food & Machinery, 2019, 35(10): 117-121, 183.
[14] SONG Q B, XIA X, JI C M, et al. Optimized flash extraction and UPLC-MS analysis on antioxidant compositions of Nitraria sibirica fruit[J]. Journal of Pharmaceutical and Biomedical Analysis, 2019, 172: 379-387.
[15] HAO Y J, ZHANG K X, JIN M Y, et al. Improving fed-batch culture efficiency of Rhodiola sachalinensis cells and optimizing flash extraction process of polysaccharides from the cultured cells by BBD-RSM[J]. Industrial Crops and Products, 2023, 196: 116513.
[16] 沈晓静, 解富娟, 周绍琴, 等. 咖啡生豆多糖提取及抗氧化活性[J]. 食品与机械, 2023, 39(5): 144-149, 181. SHEN X J, XIE F J, ZHOU S Q, et al. Extraction of polysaccharide from green coffee beans and its antioxidant activity[J]. Food & Machinery, 2023, 39(5): 144-149, 181.
[17] 清源, 陈甜甜. 块菌多糖复合酶法提取工艺优化[J]. 食品与机械, 2018, 34(10): 174-178. QING Y, CHEN T T. Optimization of the extraction of truffle polysaccharide by multi-enzyme hydrolysis[J]. Food & Machinery, 2018, 34(10): 174-178.
[18] 史德芳, 高虹, 谭洪卓, 等. 香菇柄多糖的微波辅助提取及其活性研究[J]. 食品研究与开发, 2010, 31(2): 10-14. SHI D F, GAO H, TAN H Z, et al. Study on microwave-assisted extraction and biological activity of polysaccharide from Lentinus edodes stem[J]. Food Research and Development, 2010, 31(2): 10-14.
[19] 池源, 王丽波. 苯酚—硫酸法测定南瓜籽多糖含量的条件优化[J]. 食品与机械, 2014, 30(1): 89-92. CHI Y, WANG L B. Study on phenol-sulfuric acid method for determination of polysaccharide content in pumpkin seeds[J]. Food & Machinery, 2014, 30(1): 89-92.
[20] 李楠, 张香飞, 杨春杰. 板枣多糖初级结构表征及抗氧化活性[J]. 食品与机械, 2022, 38(10): 24-28, 49. LI N, ZHANG X F, YANG C J. Primary structure characterization and antioxidant activity of polysaccharide from Zizyphus jujuba cv. banzao[J]. Food & Machinery, 2022, 38(10): 24-28, 49.
[21] 姜慧, 孔立敏, 王翀, 等. 条斑紫菜多糖的降解条件优化及其生物活性[J]. 食品科学, 2021, 42(3): 38-47. JIANG H, KONG L M, WANG C, et al. Optimization of degradation conditions of polysaccharides from Porphyra yezoensis and changes in biological activities after degradation[J]. Food Science, 2021, 42(3): 38-47.
[22] CHEN B J, SHI M J, CUI S, et al. Improved antioxidant and anti-tyrosinase activity of polysaccharide from Sargassum fusiforme by degradation[J]. International Journal of Biological Macromolecules, 2016, 92: 715-722.
[23] 王苗苗, 刘宗浩, 张永,等. 2种新疆沙棘中黄酮、多酚及其抗氧化活性分析[J]. 食品工业科技, 2020, 41(18): 51-57. WANG M M, LIU Z H, ZHANG Y, et al. Analysis of the flavonoid polyphenol and its antioxidant activity of 2 kinds of sea buckthorn from Xinjiang[J]. Science and Technology of Food Industry, 2020, 41(18): 51-57.
[24] 李昌勤, 姚辰, 朱荣遥, 等. 基于DPPH、ABTS和FRAP的中药女贞子抗氧化谱效关系研究[J]. 中国中药杂志, 2016, 41(9): 1 670-1 677. LI C Q, YAO C, ZHU R Y, et al. Spectrum-effect relationship in antioxidant activity of Ligustri Lucidi Fructus based on DPPH, ABTS and FRAP assays[J]. China Journal of Chinese Materia Medica, 2016, 41(9): 1 670-1 677.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.