Abstract
Objective: To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. Methods: The HPLC method was performed on an Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5 m) with gradient elution of methanol and 0.6% phosphoric acid solution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was set at 255 nm. Results: The contents of rutin, hypericin and quercetin in 20 batches of lotus samples were 1.32~2.06, 1.36~2.43, 1.41~3.26 mg/kg, respectively. The linear range of the concentration of rutin, hypericin and quercetin was 5~200 g/mL (R2=0.999 9), the three flavonoids showed a good linearity, a high sensitivity, and good serviceability. The relative standard deviation (RSD) for precision, stability, repeatability, and recoveries test were all less than 4.0%, and the average recovery rate of added standard were 96.6%, 96.9%, 97.4%, respecitvely. Conclusion: The method is convenient and accurate, and can be used for the content determination and quality evaluation of flavonoids in Xiang lotus.
Publication Date
10-30-2023
First Page
57
Last Page
64
DOI
10.13652/j.spjx.1003.5788.2023.80595
Recommended Citation
Feifei, ZHONG; Jing, LI; Deqing, LEI; Yun, YANG; Jianfang, WU; and Li, OUYANG
(2023)
"Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC,"
Food and Machinery: Vol. 39:
Iss.
9, Article 9.
DOI: 10.13652/j.spjx.1003.5788.2023.80595
Available at:
https://www.ifoodmm.cn/journal/vol39/iss9/9
References
[1] 彭斯文, 黄艳宁, 沈容. 湘莲的本草考证[J]. 中国民族民间医药杂, 2015, 24(17): 13-14.
PENG S W, HUANG Y N, SHEN R. Textual research of the Nelumbo nucifera gaertn[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2015, 24(17): 13-14.
[2] 高燕菁. 荷花: 药食同用 全身是宝[N]. 中国医药报, 2016-07-15(2).
GAO Y Q. Lotus: Medicine and food with the whole body is treasure[N]. China Medical News, 2016-07-15(2).
[3] 廖立, 舒展, 李笑然, 等. 莲类药材的化学成分和药理作用研究进展[J]. 上海中医药杂志, 2010, 44(12): 82-84.
LIAO L, SHE Z, LI X R, et al. Research advance in chemical compositions and phamacological actions of medicinal herbs related to lotus[J]. Shanghai Journal of Traditional Chinese Medicine, 2010, 44(12): 82-84.
[4] 张元丽, 王素利, 张宇, 等. 金丝桃苷调控胰岛素受体底物1/磷脂酰肌醇3-激酶/蛋白激酶B信号通路对糖尿病肾病大鼠肾组织损伤的保护作用[J]. 中国临床药理学杂志, 2023, 39(2): 241-245.
ZHANG Y L, WANG S L, ZHANG Y, et al. Protective effect of hyperoside on renal tissue damage in rats with diabetic nephropathy by regulating the insulin receptor substrate 1 / phosphatidylinositol 3-kinase / protein kinase B signaling pathway[J]. Chin J Clin Pharmacol, 2023, 39(2): 241-245.
[5] 郭建军, 凌应飞, 李强. 芦丁通过抑制NF-κB信号通路缓解大鼠结肠炎的研究[J]. 安徽医药, 2023, 27(1): 46-49.
GUO J J, LING Y F, LI Q. Rutin relieves colitis in rats by inhibiting NF-κB signaling pathway[J]. Anhui Medical and Pharmaceutical Journal, 2023, 27(1): 46-49.
[6] 马纳, 李亚静, 范吉平. 槲皮素药理作用研究进展[J]. 辽宁中医药大学学报, 2018, 20(8): 221-224.
MA N, LI Y J, FAN J P. Research progress on pharmacological action of quercetin[J]. Journal of Liaoning University of Tcm, 2018, 20(8): 221-224.
[7] 刘丽娜, 李海亮, 李耀磊, 等. 银杏叶片中5种银杏双黄酮类成分同时测定方法的建立[J]. 中国药房, 2022, 33(10): 1 220-1 224.
LIU L N, LI H L, LI Y L, et al. Establishment of determination method for five Ginkgo biflavones in Ginkgo biloba leaves tablets[J]. China Pharmacy, 2022, 33(10): 1 220-1 224.
[8] 郑凯, 刘保山, 宋歌, 等. 比较荧光分光光度法和紫外—可见分光光度法检测刺葡萄叶片总黄酮含量[J]. 湖北民族大学学报(自然科学版), 2023, 41(1): 14-18, 32.
ZHENG K, LIU B S, SONG G, et al. Comparison of fluorescence spectrophotometry and uv-vis spectrophtometry for determination of total flavonoids in vitis davidii leaves[J]. Journal of Hubei Minzu University (Natural Science Edition), 2023, 41(1): 14-18, 32.
[9] 王亚, 杨清山, 徐猛, 等. 银杏叶及其提取物中银杏黄酮的HPLC测定方法研究[J]. 食品研究与开发, 2018, 39(9): 135-141.
WANG Y, YANG Q S, XU M, et al. Study on the determination method of Ginkgo flavonoids in ginkgo leaves and egb by HPLC[J]. Food Research and Development, 2018, 39(9): 135-141.
[10] 周宗洲, 张艳萍, 黄婉锋, 等. 超高效液相色谱—串联质谱法同时测定化妆品植物提取物原料中13种黄酮类化合物[J]. 广东化工, 2022, 49(22): 181-183, 196.
ZHOU Z Z, ZHANG Y P, HUANG W F, et al. Simultaneous deyermination of 13 flavonoids in cosmetic plant extracts by ultra performance liquid chromatograohy thandem mass spectrometry[J]. Guangdong Chemical Industry, 2022, 49(22): 181-183, 196.
[11] 李静, 贾文江, 曹望弟, 等. 高效液相色谱法同时测定分心木中芦丁、槲皮素、金丝桃苷含量[J]. 中国药业, 2021, 30(20): 61-63.
LI J, JIA W J, CAO W D, et al. Content determination of ruyin, quercetin and hyperosidein diaphragma juglandis fructus by HPLC[J]. Chinese Traditional Patent Medicine, 2021, 30(20): 61-63.