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Corresponding Author(s)

徐红艳（1975—），女，延边大学教授，博士。E-mail：xuhongyan@ybu.edu.cn

Abstract

Exosome-like nanoparticles （ELNs） are nano-sized extracellular vesicles enclosed by lipid bilayer membrane and secreted by cells; And carrying a variety of cargo, including lipids, proteins, miRNAs and secondary metabolites. Plant ELNs have received widespread attention due to their unique structure and excellent physiological activity. This review summarizes the isolation methods, characteristics, components and functions of plant ELNs, focusing on the application of multi-omics technologies in plant ELNs. In addition, some suggestions for further study of plant ELNs were put forward.

Publication Date

1-30-2024

First Page

226

Last Page

233

DOI

10.13652/j.spjx.1003.5788.2023.80423

References

[1] KIM J S, LI S Y, ZHANG S Y, et al. Plant-derived exosome-like nanoparticles and their therapeutic activities[J]. Asian Journal of Pharmaceutical Sciences, 2022, 17（1）: 53-69.
[2] QUESENBERRY P J, GOLDBERG L R, ALIOTTA J M, et al. Cellular phenotype and extracellular vesicles: Basic and clinical considerations[J]. Stem Cells & Development, 2014, 23（13）: 1 429-1 436.
[3] COCUCCI E, RACCHETTI G, MELDOLESI J. Shedding microvesicles: Artefacts no more[J]. Trends in Cell Biology, 2009, 19（2）: 43-51.
[4] THERY C, WITWER K, AIKAWA E, et al. Minimal information for studies of extracellular vesicles 2018 （MISEV2018）: A position statement of the international society for extracellular vesicles and update of the MISEV2014 guidelines[J]. Journal of Extracellular Vesicles, 2018, 7: 1535750.
[5] CHEN Q B, LI Q, LIANG Y Q, et al. Natural exosome-like nanovesicles from edible tea flowers suppress metastatic breast cancervia ROS generation and microbiota modulation[J]. Acta Pharmaceutica Sinica B, 2022, 12（2）: 907-923.
[6] ZHANG L, HE F J, GAO L N, et al. Engineering exosome-like nanovesicles derived from Asparagus cochinchinensiscan Inhibit the proliferation of hepatocellular carcinoma cells with better safety profile[J]. International Journal of Nanomedicine, 2021, 26（16）: 1 575-1 586.
[7] DENG Z B, RONG Y, TENG Y, et al. Broccoli-derived nanoparticle inhibits mouse colitis by activating dendritic cell AMP-activated protein kinase[J]. Molecular Therapy, 2017, 25（7）: 1 641-1 654.
[8] GARAEVAL L, KAMYSHINSKY R, KIL Y, et al. Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro[J]. Scientific Reports, 2021, 11: 6 489.
[9] HE B Y, HAMBY R, JIN H L. Plant extracellular vesicles: Trojan horses of cross-kingdom warfare[J]. FASEB Bioadvances, 2021, 3（9）: 657-664.
[10] XU X H, YUAN T J, DAD A H, et al. Plant exosomes as novel nanoplatforms for microRNA transfer stimulate neural differentiation of stem cells in vitro and in vivo[J]. Nano Letters, 2021, 21（19）: 8 151-8 159.
[11] KILASONIYA A, GARAEVA L, SHTAM T, et al. Potential of plant exosome vesicles from grapefruit （Citrus × paradisi） and tomato （Solanum lycopersicum） juices as functional ingredients and targeted drug delivery vehicles[J]. Antioxidants （Basel）, 2023, 12（4）: 943.
[12] YANG D, ZHANG W, ZHANG H, et al. Progress, opportunity, and perspective on exosome isolation-efforts for efficient exosome-based theranostics[J]. Theranostics, 2020, 10（8）: 3 684-3 707.
[13] CHEN J, LI P, ZHANG T, et al. Review on strategies and technologies for exosome isolation and purification[J]. Frontiers in Bioengineering and Biotechnology, 2022, 9: 811971.
[14] CONG M H, TAN S Y, LI S M, et al. Technology insight: Plant-derived vesicles-how far from the clinical biotherapeutics and therapeutic drug carriers?[J]. Advanced Drug Delivery Reviews, 2022, 182: 114108.
[15] LIVSHITS M A, KHOMYAKOVA E, EVTUSHENKO E G, et al. Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol[J]. Scientific Reports, 2015, 5: 17319.
[16] LI S M, HE F J, QIN L X, et al. Preparation and properties of extracellular vesicle-like nanoparticles from medicinal and edible plants[J]. Acta Pharmaceutica Sinica, 2021, 12: 2 086-2 092.
[17] BALDINI N, TORREGGIANI E, RONCUZZI L, et al. Exosome-like nanovesicles isolated from Citrus limon L. exert antioxidative effect[J]. Current Pharmaceutical Biotechnology, 2018, 19 （11）: 877-885.
[18] WANG B M, ZHUANG X Y, DENG Z B, et al. Targeted drug delivery to intestinal macrophages by bioactive nanovesicles released from grape fruit[J]. Molecular Therapy, 2014, 22: 522-534.
[19] ZHUANG X Y, DENG Z B, MU J Y, et al. Ginger-derived nanoparticles protect against alcohol-induced liver damage[J]. Journal of Extracellular Vesicles, 2015, 4: 28713.
[20] ZHANG M Z, VIENNOIS E, PRASAD M, et al. Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer[J]. Biomaterials, 2016, 101: 321-340.
[21] ZU M H, XIE D C, BRANDON S B, et al. "Green" nanotherapeutics from tea leaves for orally targeted prevention and alleviation of colon diseases[J]. Biomaterials, 2021, 279: 121178.
[22] XIAO J, FENG S Y, WANG X, et al. Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables[J]. Peer J, 2018, 6: e5186.
[23] STANLY C, MOUBARAK M, FIUME I, et al. Membrane transporters in citrus clementina fruit juice-derived nanovesicles[J]. International Journal of Molecular Sciences, 2019, 20（24）: e6205.
[24] JU S W, MU J Y, DOCKLAND T, et al. Grape exosome-like nanoparticles induce intestinal stem cells and protect mice from DSS-induced colitis[J]. Molecular Therapy, 2013, 21（7）: 1 345-1 357.
[25] WANG Q L, REN Y, MU J Y, et al. Grape fruit-derived nanovectors use an activated leukocyte trafficking pathway to deliver therapeutic agents to inflammatory tumor sites[J]. Cancer Research, 2015, 75（12）: 2 520-2 529.
[26] BRAHMBHATT M, GUNDALA S R, ASIF G, et al. Ginger phytochemicals exhibit synergy to inhibit prostate cancer cell proliferation[J]. Nutrition and Cancer, 2013, 65（2）: 263-272.
[27] MU J Y, ZHUANG X Y, WANG Q L, et al. Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles[J]. Molecular Nutrition & Food Research, 2014, 58（7）: 1 561-1 573.
[28] ZHAO Z H, YU S R, LI M, et al. Isolation of exosome-like nanoparticles and analysis of microRNAs derived from coconut water based on small RNA high-throughput sequencing[J]. Journal of Agricultural and Food Chemistry, 2018, 66 （11）: 2 749-2 757.
[29] YU S R, ZHAO Z H, XU X Y, et al. Characterization of three different types of extracellular vesicles and their impact on bacterial growth[J]. Food Chemistry, 2019, 272: 372-378.
[30] FUJITA D, ARAI T, KOMORI H, et al. Apple-derived nanoparticles modulate expression of Organic-anion-transporting polypeptide （OATP） 2B1 in caco-2 cells[J]. Molecular Pharmaceutics, 2018, 15 （12）: 5 772-5 780.
[31] LU S Y, YANG S, REN L M, et al. Panax ginseng exosomes promote HaCaT cell proliferation and wound healing[J]. Chinese Journal of Biochemistry and Molecular Biology, 2021, 37（11）: 1 510-1 519.
[32] ZHAO W J, BIAN Y P, WANG Q H, et al. Blueberry-derived exosomes-like nanoparticles ameliorate nonalcoholic fatty liver disease by attenuating mitochondrial oxidative stress[J]. Acta Pharmacologica Sinica, 2022, 43: 645-658.
[33] WANG Y, WEI Y S, LIAO H, et al. Plant exosome-like nanoparticles as biological shuttles for transdermal drug delivery[J]. Bioengineering （Basel）, 2023, 10（1）: 104.
[34] ZHANG L, LI S M, CONG M H, et al. Lemon-derived extracellular vesicle-like nanoparticles block the progression of kidney stones by antagonizing endoplasmic reticulum stress in renal tubular cells[J]. Nano Letters, 2023, 23（4）: 1 555-1 563.
[35] WOLFRAM J, ZHU M T, YANG Y, et al. Safety of nanoparticles in medicine[J]. Current Drug Targets, 2015, 16（14）: 1 671-1 681.
[36] NELEMANS L C, GUREVICH L. Drug delivery with polymeric nanocarriers-cellular uptake mechanisms[J]. Materials, 2020, 13（2）: 366.
[37] ZHOU M X, HUANG H, WANG D Q, et al. Light-triggered PEGylation/de PEGylation of the nanocarriers for enhanced tumor penetration[J]. Nano Letters, 2019, 19（6）: 3 671-3 675.
[38] WANG Q L, ZHUANG X Y, MU J Y, et al. Delivery of therapeutic agents by nanoparticles made of grape fruit-derived lipids[J]. Nature Communications, 2013, 4: 1 867.
[39] ZHANG M Z, XIAO B, WANG H, et al. Edible ginger-derived nano-lipids loaded with doxorubicin as a novel drug-delivery approach for colon cancer therapy[J]. Molecular Therapy, 2016, 24: 1 783-1 796.
[40] KIM M K, CHOI Y C, CHO S H, et al. The antioxidant effect of small extracellular vesicles derived from Aloe vera peels for wound healing[J]. Tissue Engineering and Regenerative Medicine, 2021, 18（4）: 561-571.
[41] SUNDARAM K, MILLER D P, KUMAR A, et al. Plant-derived exosomal nanoparticles inhibit pathogenicity of Porphyromonas gingivalis[J]. iScience, 2019, 22（21）: 308-327.
[42] SONG H L, CANUP B S B, NGO V L, et al. Internalization of garlic-derived nanovesicles on liver cells is triggered by interaction with CD98[J]. ACS Omega, 2020, 5: 23 118-23 128.
[43] CAI Q, QIAO L L, WANG M, et al. plants send small RNAs in extracellular vesicles to fungal pathogen to silence virulence genes[J]. Science, 2018, 360: 1 126-1 129.
[44] GUDBERGSSON J M, JNSSON K, SIMONSEN J B, et al. Systematic review of targeted extracellular vesicles for drug delivery-Considerations on methodological and biological heterogeneity[J]. Journal of Controlled Release, 2019, 28（306）: 108-120.
[45] RAIMONDO S, NASELLI F, FONTANA S, et al. Citrus limon-derived nanovesicles inhibit cancer cell proliferation and suppress CML xenograft growth by inducing TRAIL-mediated cell death[J]. Oncotarget, 2015, 6（23）: 19 514-19 527.
[46] KALARIKKAL S P, SUNDARAM G M. Edible plant-derived exosomal microRNAs: Exploiting a cross-kingdom regulatory mechanism for targeting SARS-CoV-2[J]. Toxicology and Applied Pharmacology, 2021, 414: 115425.
[47] BOKKA R, RAMOS A P, FIUME I, et al. Biomanufacturing of tomato-derived nanovesicles[J]. Foods, 2020, 9: 1 852.
[48] SUNDARAM K, MU J, KUMAR A, et al. Garlic exosome-like nanoparticles reverse high-fat diet induced obesity via the gut/brain axis[J]. Theranostics, 2022, 12（3）: 1 220-1 246.
[49] CHEN X Y, ZHOU Y, YU J J. Exosome-like nanoparticles from ginger rhizomes inhibited NLRP3 inflammasome activation[J]. Molecular Pharmaceutics, 2019, 16: 2 690-2 699.
[50] LI C M, SONG Q, YIN X L, et al. Preparation, characterization, and in vitro anticancer activity evaluation of broccoli-derived extracellular vesicle-coated astaxanthin nanoparticles[J]. Molecules, 2022, 27: 3 955.
[51] CAO M, YAN H J, HAN X, et al. Ginseng-derived nanoparticles alter macrophage polarization to inhibit melanoma growth[J]. Journal for ImmunoTherapy of Cancer, 2019, 7（1）: 326.
[52] RAIMONDO S, URZ O, MERAVIGLIA S, et al. Anti-inflammatory properties of lemon-derived extracellular vesicles are achieved through the inhibition of ERK/NF-κB signalling pathways[J]. Journal of Cellular and Molecular Medicine, 2022, 26（15）: 4 195-4 209.
[53] TENG Y, REN Y, SAYED M, et al. Plant-derived exosomal microRNAs shape the gut microbiota[J]. Cell Host Microbe, 2018, 24: 637-652.
[54] PERUT F, RONCUZZI L, AVNET S, et al. Strawberry-derived exosome-like nanoparticles prevent oxidative stress in human mesenchymal stromal cells[J]. Biomolecules, 2021, 11（1）: 87.
[55] CHEN X Y, LIU B L, LI X Z, et al. Identification of anti-inflammatory vesicle-like nanoparticles in honey[J]. Journal of Extracellular Vesicles, 2021, 10（4）: e12069.
[56] TENG Y, XU F Y, ZHANG X C, et al. Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12[J]. Molecular Therapy, 2021, 29: 2 424-2 440.
[57] KOMORI H, FUJITA D, SHIRASAKI Y, et al. MicroRNAs in apple-derived nanoparticles modulate intestinal expression of organic anion-transporting peptide 2B1/SLCO2B1 in caco-2 Cells[J]. Drug Metabolism and Disposition, 2021, 49（9）: 803-809.
[58] MARTNEZ-BALLESTA M D C, GARCA-GOMEZ P, YEPES-MOLINA L, et al. Plasma membrane aquaporins mediates vesicle stability in broccoli[J]. PLoS One, 2018, 13（2）: e0192422.
[59] YANG M, LUO Q Q, CHEN X, et al. Bitter melon derived extracellular vesicles enhance the therapeutic effects and reduce the drug resistance of 5-fluorouracil on oral squamous cell carcinoma[J]. Journal of Nanobiotechnology, 2021, 19: 259.
[60] SRIWASTVA M K, DENG Z B, WANG B, et al. Exosome-like nanoparticles from mulberry bark prevent DSS-induced colitis via the AhR/COPS8 pathway[J]. EMBO Reports, 2022, 23（3）: e53365.
[61] PREZ-BERMDEZ P, BLESA J, SORIANO J M, et al. Extracellular vesicles in food: Experimental evidence of their secretion in grape fruits[J]. European Journal of Pharmaceutical Sciences, 2017, 98: 40-50.
[62] WOITH E, GUERRIERO G, HAUSMAN J F, et al. Plant extracellular vesicles and nanovesicles: Focus on secondary metabolites, proteins and lipids with perspectives on their potential and sources[J]. International Journal of Molecular Sciences, 2021, 22: 3 719.
[63] CHALBI N, MARTNEZ-BALLESTA M C, YOUSSEF N B, et al. Intrinsic stability of brassicaceae plasma membrane in relation to changes in proteins and lipids as a response to salinity[J]. Journal of Plant Physiology, 2015, 175: 148-156.
[64] DELIMA R M T, DOS R A C, DE MENEZES A P M, et al. Protective and therapeutic potential of ginger （Zingiber officinale） extract and[6]-gingerol in cancer: A comprehensive review[J]. Phytotherapy Research: PTR, 2018, 32（10）: 1 885-1 907.
[65] BISCHOFF-KONT I, FURST R. Benefits of ginger and its constituent 6-shogaol in inhibiting inflammatory processes[J]. Pharmaceuticals, 2021, 14: 571.

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