Abstract
Objective: This study aimed to establish a method for the simultaneous determination of 16 quinolones in freshwater fish by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and then analyze the effect of three different cooking methods (by boiling, steaming and frying) on the residues of quinolone antibiotics. Methods: Samples were ultrasonic extracted with acetonitrile (with 4% formic acid) and purified with C18 solid phase extraction column. The chromatographic separation was performed on a Waters ACQUITY UPLC BEH C18 (1.7 μm, 2.1 mm×100 mm) column, with 0.1% formic acid water-acetonitrile as mobile phase for gradient elution. Mass spectrometry (ESI+) was monitored by multiple reaction ions and quantified by internal standard method. Results: 16 quinolones showed a clear linear relationship between 2.0 ng/mL and 50 ng/mL, and the correlation coefficient was greater than 0.998 49. The limits of detection quantification were 0.3~4.1 μg/kg and 0.9~11.0 μg/kg, respectively. Average recoveries in matrices at low, medium, and high spiked levels ranged from 79.0% to 104.7%, with a standard deviation of 1.0% to 8.1%. There was no significant difference in the residues of 16 quinolones between the fish meat-positive samples of three different cooking methods and the control. Conclusion: The method was simple, rapid, and sensitive, and met the high-throughput screening and dietary risk assessment of quinolone antibiotics in freshwater fish.
Publication Date
1-30-2024
First Page
40
Last Page
46,54
DOI
10.13652/j.spjx.1003.5788.2022.80745
Recommended Citation
Yunkai, GAO; Chunqian, CHENG; Xiaomei, CHENG; Lingyu, ZHOU; Yinglin, DEND; and Qingchun, YIN
(2024)
"Establishment and dietary risk assessment of UPLC-MS/MS detection method for 16 quinolone antibiotics in freshwater fish,"
Food and Machinery: Vol. 40:
Iss.
1, Article 6.
DOI: 10.13652/j.spjx.1003.5788.2022.80745
Available at:
https://www.ifoodmm.cn/journal/vol40/iss1/6
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