Abstract
[[Objective ]] This study aimed to estimate the measurement uncertainty of coliforms in drinking water according to ISO 19036:2019.[[Methods ]] By designing protocols,the technical uncertainty (utech),matrix uncertainty (umatrix) and distribution uncertainty (udistrib) were estimated respectively,and the combined uncertainty (uc(y)) and extended uncertainty (U) were calculated by combining the values of the three components.[[Results]] After estimating the measurement uncertainty of the second method of GB 4789.3—2016,the technical uncertainty was 0.120 2 log(CFU/mL),the matrix uncertainty was 0.1 log(CFU/mL),and the distribution uncertainty was 0.065 5 log(CFU/mL) and 0.026 1 log(CFU/mL).When the result was 440 CFU/mL,the extended uncertainty was 0.34 log(CFU/mL),expressed as (2.64±0.34) log(CFU/mL).[[Conclusion ]] According to ISO 19036:2019,the estimation of measurement uncertainty for quantitative determinations of microorganisms in food is more scientific and convenient compared to current domestic standards.
Publication Date
2-18-2025
First Page
68
Last Page
72
DOI
10.13652/j.spjx.1003.5788.2024.80113
Recommended Citation
Wentao, ZHONG; Lin, LU; Zheng, LI; Peihua, ZHOU; Zhenbai, HONG; and Cong, ZHOU
(2025)
"Estimation of measurement uncertainty of coliforms in drinking water according to ISO 19036 :2019,"
Food and Machinery: Vol. 40:
Iss.
10, Article 10.
DOI: 10.13652/j.spjx.1003.5788.2024.80113
Available at:
https://www.ifoodmm.cn/journal/vol40/iss10/10
References
[1] 国家市场监督管理总局,国家标准化管理委员会.检测和校准实验室能力的通用要求:GB/T 27025—2019 [S].北京:中国标准出版社,2019:1-20.State Administration for Market Regulation,Standardization Administration of China.General requirements for the competence of testing and calibration laboratories:GB/T 27025—2019 [S].Beijing:Standards Press of China,2019:1-20.
[2] 国家市场监督管理总局.检验检测机构资质认定评审准则[Z].2023 -05-30.State Administration for Market Regulation.Rules for the review of qualification accreditation of inspection and testing institutions [Z].2023 -05-30.
[3] DIAS F R S,LOURENÇO F R.Top-down evaluation of the matrix effects in microbial enumeration test uncertainty [J].Journal of Microbiological Methods,2020,171:105864.
[4] SINGH K A,RAI R,NAIR S S.Review on development of assigned value microbiological reference materials used in food testing [J].Food Microbiology,2022,102:103904.
[5] 许瑞清,赵海霞,武瑞杰,等.整体法评定食品中大肠菌群的测量不确定度 [J].食品工程,2023 (3):40-43.XU R Q,ZHAO H X,WU R J,et al.Uncertainty in measurement of coliform in food by holistic method [J].Food Engineering,2023 (3):40-43.
[6] 田浩,李光耀,张凡,等.饮用水中铜绿假单胞菌检测结果的不确定度评定 [J].现代食品,2021 (3):206-209.TIAN H,LI G Y,ZHANG F,et al.Evaluation of uncertainty of detection results of Pseudomonas aeruginosa in drinking water[J].Modern Food,2021 (3):206-209.
[7] 魏敏,姜华军,王言爽,等.食品中霉菌和酵母计数的不确定度评定 [J].食品安全导刊,2023 (11):111-113.WEI M,JIANG H J,WANG Y S,et al.Uncertainty evaluation of the enumeration of molds and yeasts in food [J].China Food Safety Magazine,2023 (11):111-113.
[8] 游元丁,赵阳,林雪丹,等.食品中金黄色葡萄球菌测量不确定度评定 [J].食品安全质量检测学报,2021,12(6):2 468-2 473.YOU Y D,ZHAO Y,LIN X D,et al.Evaluation of uncertainty in measurement of Staphylococcus aureus in food [J].Journal of Food Safety and Quality,2021,12(6):2 468-2 473.
[9] 胡雪梅,李卫春,于芳,等.生鲜乳中菌落总数的不确定度测量[J].草食家畜,2023 (3):37-41.HU X M,LI W C,YU F,et al.Uncertainty evaluation of total bacterial colonies in fresh milk [J].Grass-Feeding Livestock,2023 (3):37-41.
[10] 赵琪,甄珍.单核细胞增生李斯特氏菌 MPN法结果测量不确定度评定 [J].中国口岸科学技术,2022,4(5):77-81.ZHAO Q,ZHEN Z.Uncertainty assessment of results measurement of Listeria monocytogenes by MPN method [J].China Port Science and Technology,2022,4(5):77-81.
[11] RENATO COUTO A,REBELLO LOURENÇO F.Bottom-up and top-down measurement uncertainty evaluation for multivariate spectrophotometric procedures [J].Microchemical Journal,2023,193:109194.
[12] BURR T,CROFT S,FAVALLI A,et al.Bottom-up and top-down uncertainty quantification for measurements [J].Chemometrics and Intelligent Laboratory Systems,2021,211:104224.
[13] LEE J H,CHOI J H,YOUN J S,et al.Comparison between bottom-up and top-down approaches in the estimation of measurement uncertainty [J].Clinical Chemistry and Laboratory Medicine (CCLM ),2015,53(7):1 025-1 032.
[14] DIAS F R S,LOURENÇO F R.Measurement uncertainty evaluation and risk of false conformity assessment for microbial enumeration tests [J].Journal of Microbiological Methods,2021,189:106312.
[15] WEISSFELD A S.Estimation of uncertainty of measurement in microbiology [J].Clinical Microbiology Newsletter,2010,32(22):171-175.
[16] 弓耀忠,王媛慧,张婷.微生物不确定度的理论与实践 [J].食品工程,2022 (4):77-79.GONG Y Z,WANF Y H,ZHANG T.Theory and practice of microbial uncertainty [J].Food Engineering,2022 (4):77-79.
[17] 王瑞兴,王礼霞,胡巅.食品微生物定量检测的测量不确定度评估指南之商榷 [J].中国卫生检验杂志,2023,33(5):638-640.WANG R X,WANG L X,HU D.Discussion on the evaluation guidelines for measurement uncertainty in quantitative detection of food microorganisms [J].Chinese Journal of Health Laboratory Technology,2023,33(5):638-640.