Abstract
［［Objective ］］ This study aimed to investigate the effects of an acidic tea polysaccharide extracted from Wuyi Rock tea "yellow slice" on bile acids in hyperglycaemic rats.［［Methods ］］ In vitro experiments simulating artificial gastric and intestinal fluids were conducted to evaluate the binding capacity of tea polysaccharides to bile salts.The composition of bile acids in the cecal contents was analyzed using Liquid Chromotography with Mass Spectrometry (LC-MS);The relative mRNA expression of bile acid receptors FXR and TGR 5 in rat liver and pancreas of rats were measured,along with Western blot analysis of colonic tissues.［［Results］］ The in vitro experiments demonstrated that tea polysaccharides possess a significant binding capacity for both bound and free bile salts,with stronger affinity for bound bile salts.At 2.00 mg/mL,the binding rate of the tea polysaccharides to sodium cholate were found to be 57.59% and 62.62% for sodium glycylcholate and sodium taurocholate,respectively.In the cecal contents of rats,intervention with tea polysaccharides primarily increased the level of secondary bile acids,including deoxycholic acid and lithocholic acid.Additionlly,the treatment upregulated the mRNA expression of bile acid receptor FXR and TGR 5 in the liver and pancreas,with no significant change observed in the expression of GLP -1.［［Conclusion ］］ The intervention with acidic tea polysaccharides from Wuyi rock tea led to an increased ratio of primary bile acids to secondary bile acids.This suggests that the more hydrophobic secondary bile acids are less effectively absorbed in the enterohepatic circulation.
Publication Date
2-18-2025
First Page
129
Last Page
135
DOI
10.13652/j.spjx.1003.5788.2024.80090
Recommended Citation
Zhong, WU and Jiangfan, YANG
(2025)
"The regulatory mechanism of acidic tea polysaccharides from Wuyi rock tea on bile acids in type 2 diabetic rats,"
Food and Machinery: Vol. 40:
Iss.
10, Article 19.
DOI: 10.13652/j.spjx.1003.5788.2024.80090
Available at:
https://www.ifoodmm.cn/journal/vol40/iss10/19
References
[1] WAHLSTRÖM A,SAYIN S I,MARSCHALL H U,et al.Intestinal crosstalk between bile acids and microbiota and its impact on host metabolism [J].Cell Metabolism,2016,24(1):41-50.
[2] JOYCE S A,MACSHARRY J,CASEY P G,et al.Regulation of host weight gain and lipid metabolism by bacterial bile acid modification in the gut [J].Proceedings of the National Academy of Sciences,2014,111(20):7 421-7 426.
[3] PUSHPASS R A,ALZOUFAIRI S,JACKSON K G,et al.Circulating bile acids as a link between the gut microbiota and cardiovascular health:impact of prebiotics,probiotics and polyphenol-rich foods [J].Nutrition Research Reviews,2022,35(2):161-180.
[4] 石玉涛,李照莹,周巧芳,等.武夷岩茶副产品黄片再加工工##表示 DC组与 NC组之间差异极显著 （P<0.01）；*表示茶多糖组与 DC组之间差异显著 （P<0.05）|艺优化 [J].武夷学院学报,2018,37(9):17-21.SHI Y T,LI Z Y,ZHOU Q F,et al.Optimization of the reprocessing technology for byproduct of Wuyi rock-essence tea[J].Journal of Wuyi University,2018,37(9):17-21.
[5] WU Z,ZENG W Z,ZHANG X,et al.Characterization of acidic tea polysaccharides from yellow leaves of Wuyi rock tea and their hypoglycemic activity via intestinal flora regulation in rats[J].Foods,2022,11(4):617-647.
[6] LIU X X,ZHANG Y H,LI W H,et al.Fucoidan ameliorated dextran sulfate sodium-induced ulcerative colitis by modulating gut microbiota and bile acid metabolism [J].Journal of Agricultural and Food Chemistry,2022,70(47):14 864-14 876.
[7] HUANG F J,ZHENG X J,MA X H,et al.Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism [J].Nature Communication,2019,10(1):1-17.
[8] 代丽凤,蒋洁琳,官兴丽,等.茶褐素理化性质及生物学活性研究进展 [J].食品与机械,2023,39(6):227-233,240.DAI L F,JIANG J L,GUAN X L,et al.Research progress on physicochemical properties and biological activity of theabrownin [J].Food & Machinery,2023,39(6):227-233,240.
[9] XIAO Y,LI M Y,WU Y P,et al.Structural characteristics and hypolipidemic activity of theabrownins from dark tea fermented by single species eurotium cristatum PW- 1[J].Biomolecules,2020,10(2):204.
[10] 纪秀凤,刘海春,吕长鑫,等.红树莓籽原花青素提取及其结合胆酸盐能力评价 [J].食品研究与开发,2019,40(7):90-97.JI X F,LIU H C,LU C X,et al.Extraction of proanthocyanidin from red raspberry seed and evaluation of its capacities of binding bile salts [J].Food Research and Development,2019,40(7):90-97.
[11] 刘爽爽,刘东超,孙凡越,等.粗毛纤孔菌总三萜的纯化及对胆酸盐结合能力分析 [J].中国农业大学学报,2020,25(5):104-113.LIU S S,LIU D C,SUN F Y,et al.Purification of total triterpenoids from Inonotus hispidus and analysis on bile salt binding ability [J].Journal of China Agricultural University,2020,25(5):104-113.
[12] 曾桥,韦承伯,缑会莉,等.杜仲叶茯砖茶多糖提取工艺优化及抗氧化降血脂活性 [J].食品科技,2018,43(8):184-192.ZENG Q,WEI C B,GOU H L,et al.Optimization of the extraction process and evaluation of antioxidant activities and hypolipidemic effects of polysaccharides from Eucommia ulmoides leaves Fu brick tea [J].Food Science and Technology,2018,43(8):184-192.[13] 王猛猛,俞蕴莉,屈昱晨,等.LC-MS/MS 测定大鼠血清中 15种胆汁酸成分 [J].中国现代应用药学,2020,37(9):1 030-1 034.WANG M M,YU Y L,QU Y C,et al.Determination of fifteen bile acids concentration in rats ’ serum by LC-MS/MS [J].Chinese Journal of Modern Applied Pharmacy,2020,37(9):1 030-1 034.
[14] FENG Z Q,DOU W,ALAXI S,et al.Modified soluble dietary fiber from black bean coats with its rheological and bile acid binding properties [J].Food Hydrocolloids,2017,62(3):94-101.
[15] STORY J,KRITCHEVSKY D.Comparison of the binding of various bile acids and bile salts in vitro by several types of fiber [J].Journal of Nutrition,1976,106(9):1 292-1 294.
[16] 华梅,樊美玲,卢佳希,等.七种药食两用中药膳食纤维体外抗氧化及胆酸盐结合能力研究 [J].食品工业科技,2021,42(11):314-320.HUA M,FAN M L,LU J X,et al.Study on the in vitro antioxidant and bile salts binding activity of the dietary fibers from seven edible traditional Chinese medicines [J].Science and Technology of Food Industry,2021,42(11):314-320.
[17] CARIOU B,CHETIVEAUX M,ZAÏR Y,et al.Fasting plasma chenodeoxycholic acid and cholic acid concentrations are inversely correlated with insulin sensitivity in adults [J].Nutrition & Metabolism,2011,8(1):48-53.
[18] TIAN S Y,CHEN S M,PAN C X,et al.FXR:structures,biology,and drug development for NASH and fibrosis diseases[J].Acta Pharmacologica Sinica,2022,43(5):1 120-1 132.
[19] ZHANG H M,WANG X,WU Z H,et al.Beneficial effect of farnesoid X receptor activation on metabolism in a diabetic rat model [J].Molecular Medicine Reports,2016,13(3):2 135-2 142.
[20] EJELONU O C,ELEKOFEHINTI O O,ADANLAWO I G,et al.TGR 5 potentiates GLP- 1 secretion and cause pancreatic islet regeneration in response to Tithonia diversifolia saponin-rich extract in diabetic model mice [J].Phytomedicine Plus,2022,2(1):1-7.
[21] HOLTER M M,CHIRIKJIAN M K,GOVANI V N,et al.TGR 5 signaling in hepatic metabolic health [J].Nutrients,2020,12(9):2 598-2 612.
[22] KOH A,VADDER D F,KOVATCHEVA-DATCHARY P,et al.From dietary fiber to host physiology:short-chain fatty acids as key bacterial metabolites [J].Cell,2016,165(6):1 322-1 345.
