•  
  •  
 

Corresponding Author(s)

洪键（1981—），男，盐城师范学院高级实验师，博士。E-mail：jian_hong602@sina.com

Abstract

［［Objective ］］ This study aimed to clarify the functional components and active effects of Cynanchum auriculatum Royle ex Wight leaves,and make use of C.auriculatum Royle ex Wight leaves as resources.［［Methods ］］ Taking the leaves of C.auriculatum Royle ex Wight at different growth stages as the research material,the changes of the main functional components in the leaves of C.auriculatum Royle ex Wight were measured in three different stages:germination stage (May ),growth stage (August ) and deciduous stage (October ).The antioxidant capacity and hypoglycemic activity of the leaves were analyzed and compared,and the leaves were used to make a leaf tea with health care functions.［［Results］］ The polysaccharide content and C21 steroid glycoside content in the leaves of C.auriculatum Royle ex Wight in May,August and October were 13.803,3.064 and 3.809 mg/g,respectively,which were significantly lower than those in the roots of C.auriculatum Royle ex Wight.The flavonoid contents were 13.407,17.776 and 2.022 mg/g,respectively,which were significantly higher than those of C.auriculatum Royle ex Wightroot.The inhibition rate of α-glucosidase and antioxidant capacity of August leaf extract were higher than those oftuber.C.auriculatum Royle ex Wight leaf tea has high content of active substances,which has antioxidant and hypoglycemic activities.［［Conclusion ］］ The C.auriculatum Royle ex Wight leaves have high functional components and bioactive substances,making them highly valuable for use.

Publication Date

2-18-2025

First Page

148

Last Page

155

DOI

10.13652/j.spjx.1003.5788.2024.60012

References

[1] CHEN W H,ZHANG Z Z,BAN Y F,et al.Cynanchum bungei Decne and its two related species for "Baishouwu":a review on traditional uses,phytochemistry,and pharmacological activities[J].Journal of Ethnopharmacology,2019,243:1-23.
[2] 张明,沈明晨,陈镭,等.白首乌的化学成分、药理作用及栽培技术综述 [J].江苏农业科学,2022,50(2):22-29.ZHANG M,SHEN M C,CHEN L,et al.On chemical constituent,pharmacological effect and cultivation techniques of Cynanchum bungei:a review [J].Jiangsu Agricultural Sciences,2022,50(2):22-29.
[3] 陈亚,徐晓燕.白首乌的生物学特性及标准化栽培技术研究[J].时珍国医国药,2014,25(7):1 730-1 731.CHEN Y,XU X Y.Research on biological characteristics and standardized cultivation techniques of Cynanchum auriculatum[J].Lishizhen Medicine and Materia Medica Research,2014,25(7):1 730-1 731.
[4] 汤俊杰,谷巍,钱旭东,等.江苏道地药材滨海白首乌的药用价值与资源评估研究 [J].南京中医药大学学报,2023,39(8):794-800.TANG J J,GU W,QIAN X D,et al.Study on the medicinal value and resource evaluation of Jiangsu genuine medicinal materials Binhai Baishouwu [J].Journal of Nanjing University of Traditional Chinese Medicine,2023,39(8):794-800.
[5] 陈其燕,石云,洪键.滨海白首乌多糖的提取与药理活性研究进展 [J].现代农业科技,2020 (19):215-216,220.CHEN Q Y,SHI Y,HONG J.Research progress on extraction and pharmacological activities of polysaccharides in Cynanchum auriculatum [J].Modern Agricultural Science and Technology,2020 (19):215-216,220.[6] 卜京,石云,林燕,等.滨海白首乌发酵片工艺优化及抗氧化活性研究 [J].食品与机械,2024,40(2):204-212.BU J,SHI Y,LIN Y,et al.Study on process optimization and antioxidant activity of fermentative Cynanchum auriculatum tablet [J].Food & Machinery,2024,40(2):204-212.
[7] 赵胤鹏,朱志鹏,苏凯越,等.“四化联动，双链耦合 ”助力革命老区乡村特色产业高质量发展:以江苏滨海县为例 [J].南方农机,2023,54(19):104-107,147.ZHAO Y P,ZHU Z P,SU K Y,et al."Four modernizations linkage and double chain coupling" to promote high-quality development of rural characteristic industries in old revolutionary base areas:a case study of Binhai,Jiangsu Province [J].China Southern Agricultural Machinery,2019,54(19):104-107,147.
[8] DING Y F,PENG Z X,DING L,et al.Baishouwu extract suppresses the development of hepatocellular carcinoma via TLR 4/MyD 88/NF-κB pathway [J].Frontiers in Pharmacology,2019 (10):389-392.
[9] 王冬艳,华欣,叶记林,等.江苏地产白首乌 C21甾体苷对高血脂大鼠血脂调节和肝脏保护作用研究 [J].安徽医药,2015,19(8):1 454-1 457.WANG D Y,HUA X,YE J L,et al.Study on hyperlipemia regulation and liver protective effect in rats by Jiangsu Baishouwu C21 steroidal glycoside [J].Anhui Medical and Pharmaceutical Journal,2015,19(8):1 454-1 457.
[10] 刘泽鑫,刘畅,钱和.白首乌花多糖对小鼠酒精性肝损伤的保护作用 [J].食品研究与开发,2020,41(13):72-79.LIU Z X,LIU C,QIAN H.Protective effect of Cynanchum auriculatum flower polysaccharide on alcoholic liver injury in mice [J].Food Research and Development,2020,41(13):72-79.
[11] 赵雪.滨海白首乌蛋白和多糖的提取纯化及其活性功能的研究 [D].哈尔滨:哈尔滨商业大学,2018:45-48.ZHAO X.Extraction,purification and active function of protein and polysaccharide form Binhai's Cynanchum auriculatum Royle ex Wigh [D].Harbin:Harbin University of Commerce,2018:45-48.
[12] 李时珍.本草纲目:金陵本 [M].北京:人民卫生出版社,1977:1 289.LI S Z.Compendium of materia medica:Jinling edition [M].Beijing:The People's Health Press Co Ltd,1977:1 289.
[13] 吴立云,仓公熬,颜天华,等.白首乌茎叶总黄酮对小鼠急性化 学 性 肝 损 伤 的 保 护 作 用 [J].时 珍 国 医 国 药,2005 (7):615-616.WU L Y,CANG G A,YAN T H,et al.Protective effect of total flavonoids of Cynanchum auriculatum Royle ex Wight on acute chemical-induced liver injury in mice [J].Lishizhen Medicine and Materia Medica Research,2005 (7):615-616.
[14] 向金田,杨梅,张强林,等.沙棘叶茶除毫装置设计与优化[J].东北农业大学学报,2023,54(5):55-65,76.XIANG J T,YANG M,ZHANG Q L,et al.Design and optimization of hair removal deceive for sea-buckthorn leaf tea[J].Journal of Northeast Agricultural University,2023,54(5):55-65,76.
[15] 张瑞刚,王超越.沙棘叶茶多糖提取工艺优化及体外降脂活性研究 [J].北方园艺,2024 (1):93-99.ZHANG R G,WANG C W.Optimization of extraction process and in vitro lipid-lowering activity of seabuckthorn leaf tea polysaccharides [J].Northern Horticulture,2024 (1):93-99.
[16] 何蓉蓉,梁磊,李怡芳,等.一种包含沙棘叶纳米超细粉的美白抗敏除皱化妆品组合物:CN 202210688744.1[P].2023 -09-22.HE R R,LIANG L,LI Y F,et al.A cosmetic composition of whitening,anti-sensitive and anti-wrinkle containing Hippophae rhamnosides L.leaf nano-ultrafine powder:CN202210688744.1[P].2023 -09-22.
[17] 丁磊,李鑫洋,刘佳鑫,等.蒲公英发酵茶工艺研究 [J].食品研究与开发,2020,41(15):75-79.DING L,LI X Y,LIU J X,et al.Study on the technology of dandelion fermentation tea [J].Food Research and Development,2020,41(15):75-79.
[18] 梁贵秋,谢婷婷,高艳芳,等.3株冠突散囊菌 (Eurotium cristatum )发酵对桑叶茶品质的影响 [J].蚕业科学,2020,46(5):614-621.LIANG G Q,XIE T T,GAO Y F,et al.Effects of fermentation of three Eurotium cristatum strains on quality of Mulberry leaf tea[J].Acta Sericologica Sinica,2020,46(5):614-621.
[19] 高丽君,王汉忠,崔建华,等.白首乌可溶性多糖提取工艺研究[J].食品科学,2004 (10):178-180.GAO L J,WANG H Z,CUI J H,et al.Studies on the extraction technology of soluble polysaccharide from Radix Cynanchum bungi [J].Food Science,2004 (10):178-180.
[20] 任美燕.红薯叶黄酮超声提取工艺优化、抗氧化及抑菌研究[J].中国食品添加剂,2023,34(1):224-232.REN M Y.Optimization of ultrasonic extraction process of flavonoids from sweet potato leaves and its antioxidative and antibacterial ability [J].China Food Additives,2023,34(1):224-232.
[21] 曹明原,曾金国.高效液相色谱法同时测定首乌叶中 3个黄酮苷的含量 [J].生物化工,2022,8(5):17-23.CAO M Y,ZENG J G.Simultaneous determination of three flavonoid glycosides in the leaves of polygonum multiflorum thunb.by HPLC [J].Biological Chemical Engineering,2022,8(5):17-23.
[22] 许慧卿.滨海白首乌甾苷提取工艺优化及其抗氧化活性研究[D].南京:南京农业大学,2019:13-24.XU H Q.Optimization on the extraction of steroidal glycosides from Binhai's Cynanchum auriculatum Royle ex Wight and its antioxidant activity [D].Nanjing:Nanjing Agricultural University,2019:13-24.[23] 郭磊,资璐熙,崔琦,等.美味牛肝菌色素大孔树脂纯化工艺及 抗 氧 化 活 性 研 究 [J].食 品 与 机 械,2022,38(1):152-158,163.GUO L,ZI L X,CUI Q,et al.Purification processing of pigment from Boletus edulis with macroporous resin and its antioxidant capacity [J].Food & Machinery,2022,38(1):152-158,163.
[24] 倪雪华,王恒鹏.低共熔溶剂提取绿茶总黄酮及其抗氧化活性[J].食品与机械,2022,38(1):159-163.NI X H,WANG H P.Research on extraction of total flavonoids from green tea with deep eutectic [J].Food & Machinery,2022,38(1):159-163.
[25] 胡博,唐晓姝,陈雪梅,等.红枣枸杞浸泡酒营养成分及体外抗氧化活性分析 [J].食品与生物技术学报,2022,41(1):44-50.HU B,TANG X S,CHEN X M,et al.Analysis of nutrients and antioxidant activity of red jujube wolfberry-soaked wine [J].Journal of Food Science and Biotechnology,2022,41(1):44-50.
[26] 冯康,李爱民,吴晓磊,等.玛咖多糖的提取条件及体外活性研究 [J].食品科学技术学报,2021,39(2):48-55.FENG K,LI A M,WU X L,et al.Study on optimization of Maca polysaccharides extraction and in vitro antioxidant and hypolipidemic activities [J].Journal of Food Science and Technology,2021,39(2):48-55.
[27] 魏婉露.扇贝废弃液多糖的分离鉴定及消化吸收情况的研究[D].大连:大连海洋大学,2022:46.WEI W L.Study on separation,purification,digestion and absorption of polysaccharide from Scallop waste liquid [D].Dalian:Dalian Ocean University,2022:46.
[28] 裴月湘,娄红祥,张卫东,等.天然药物化学 [M].7版.北京:人民卫生出版社,2016:280.PEI X Y,LOU H X,ZHANG W D,et al.Natural medicinal chemistry [M].7th ed.Beijing:The People's Health Press Co Ltd,2016:280.
[29] 谢华松,爱红,邹玉冰,等.钝药野木瓜果实的抗氧化及体外降血糖活性研究 [J].中国药业,2023,32(6):42-46.XIE H S,AI H,ZOU Y B,et al.Antioxidant activity and in vitro hypoglycemic activity of the fruit of Stauntonia leucantha[J].China Pharmaceuticals,2023,32(6):42-46.
[30] 王成民.鲜叶摊放对绿茶茶品质影响的研究进展 [J].福建茶叶,2022,44(4):42-44.WANG C M.Research progress on the effect of fresh leaf spreading on green tea quality [J].Tea in Fujian,2022,44(4):42-44.
[31] 刘梦圆,崔俪丹,项希,等.微波杀青工艺对秋季绿茶的保绿降苦作用 [J].食品安全质量检测学报,2022,13(7):2 151-2 157.LIU M Y,CUI L D,XIANG X,et al.Effects of microwave fixation process on preserving green color and reducing |bitterness in autumn green tea [J].Journal of Food Safety & Quality,2022,13(7):2 151-2 157.
[32] DI P,YANG X,WAN M M,et al.Integrative metabolomic and transcriptomic reveals potential mechanism for promotion of ginsenoside synthesis in Panax ginseng leaves under different light intensities [J].Frontiers in Bioengineering and Biotechnology,2023,11:1298501.
[33] 孙倩.白首乌酚类物质分离纯化及抗氧化活性研究 [D].南京:南京理工大学,2021:4-7.SUN Q.Study on separation,purification and antioxidant activity of phenolic from Cynanchum auriculatum Royle ex Wight [D].Nanjing:Nanjing University of Science and Technology,2021:4-7.
[34] LU M,SU H Y,LI M L,et al.Effect of UV-B radiation on growth,flavonoid and podophyllotoxin accumulation,and related gene expression in Sinopodophyllum hexandrum [J].Plant Biology,2021,23:202-209.
[35] 印鑫.白首乌 C-21甾苷对肝肾纤维化大鼠的干预作用及机制研究 [D].南京:南京中医药大学,2020:45-54.YIN X.The effect and the mechanism of total C- 21 steroridal glycosides of Baishouwu on hepatic and renal fibrosis in rats[D].Nanjing:Nanjing University of Chinese Medicine,2020:45-54.
[36] 詹鑫,陈李璟,廖广凤,等.萝藦科药用植物中新 C21甾体的研究进展 (Ⅰ) [J].广西师范大学学报 (自然科学版 ),2021,39(5):1-29.ZHAN X,CHEN L J,LIAO G F,et al.Research progress of new C21 steroids in medicinal plants of asclepiadaceae (Ⅰ) [J].Journal of Guangxi Normal University (Natural Science Edition ),2021,39(5):1-29.
[37] 殷乐,佐月,黄心意,等.玉竹根茎转录组分析及其甾体皂苷生物合成途径探究 [J].分子植物育种,2022,20(20):6 705-6 713.YIN L,ZUO Y,HUANG X Y,et al.Transcriptome analysis of Polygonatum odoratum rhizome and study on its steroid saponins biosynthesis pathway [J].Molecular Plant Breeding,2022,20(20):6 705-6 713.
[38] 王淑静,李源馨.野菊花总黄酮降血脂作用的实验研究 [J].现代食品,2017 (3):123-125.WANG S J,LI Y X.Experimental study on hyperlipidemic effect of total flavonoids [J].Modern Food,2017 (3):123-125.
[39] 何娜.紫苏叶茶加工技术研究 [D].咸阳:西北农林科技大学,2016:11-13.HE N.Study of the processing technology of Perilla leaf tea[D].Xianyang:Northwest A & F University,2016:11-13.
[40] 李亚婕,许秀松,王巧贞,等.大叶苦丁茶抗氧化活性研究进展[J].广西科学院学报,2024,40(1):21-30.LI Y J,XU X S,WANG Q Z,et al.Research progress on antioxidant activity of large-leaved Ku-dingcha [J].Journal of Guangxi Academy of Sciences,2024,40(1):21-30.
[41] 李丹,曹永,王华,等.茶叶提取物体外抗氧化活性与其功能性成分含量的相关性研究 [J].食品与机械,2018,34(6):163-168.LI D,CAO Y,WANG H,et al.Study on the relationship between the composition of tea extracts and its in vitro antioxident activity [J].Food & Machinery,2018,34(6):163-168.
[42] 邹燕,罗圣群,曾婕,等.茶多酚在医学研究中的进展 [J].福建茶叶,2023,45(6):146-148.ZOU Y,LUO S G,ZENG J,et al.Advances in the medical research of tea polyphenols [J].Tea in Fujian,2023,45(6):146-148.

Download

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.