3-Monochloropropane- 1,2-diol induced cell apoptosis in HK 2 cells via mitochondria-dependent pathway

Authors

JIN Chengni, College of Food Science , Shanxi Normal University , Taiyuan , Shanxi 030000 , China;College of Food Science and Engineering , Northwest A &F University , Xianyang , Shaanxi 712100 , China
LI Yang, College of Food Science , Shanxi Normal University , Taiyuan , Shanxi 030000 , China
YAN Shuaishuai, College of Food Science , Shanxi Normal University , Taiyuan , Shanxi 030000 , China
XU Jianguo, College of Food Science , Shanxi Normal University , Taiyuan , Shanxi 030000 , China
PENG Xiaoli, College of Food Science and Engineering , Northwest A &F University , Xianyang , Shaanxi 712100 , ChinaFollow

Corresponding Author(s)

彭晓丽（1982—），女，西北农林科技大学副教授，博士。E-mail：xiaolipeng@nwafu.edu.cn

Abstract

［Objective］ This study investigated the toxic effects of the food contaminant 3-monochloropropane -1,2-diol (3-MCPD ) on the human renal tubular epithelial cell line (HK2) and examined the underlying mechanisms.［Methods］ Cell viability,morphology,mitochondrial function,enzyme activities,and apoptosis -associated proteins expressions were assessed using MTT assay,Hoechst 33342 staining,biochemical test kits and Western blot,respectively.［Results］］ Results showed a concentration -dependent decrease in cell viability when 3-MCPD was below 80 mmol/L for 24 h.Treatment with 20 and 30 mmol/L 3-MCPD over varying durations also led to a time -dependent reduction in cell viability.Additionally,3-MCPD at 20,40 and 60 mmol/L significantly increased LDH release,decrease mitochondrial membrane potential,and lowered ATP levels.Fluorescence microscopy revealed notable morphological changes,including bright blue fluorescence,nuclear shrinkage and apoptosis bodies.Furthermore,3-MCPD treatment downregulated the anti -apoptotic protein Bcl2,upregulated the pro -apoptotic protein Bax,and increased Caspase 9 and Caspase 3 activity in a dose -dependent manner.［Conclusion］ 3-MCPD exhibits toxic effects on HK 2 cells and induces apoptosis through a mitochondria -dependent pathway.

Publication Date

2-18-2025

First Page

5

Last Page

11

DOI

10.13652/j.spjx.1003.5788.2024.80442

Recommended Citation

Chengni, JIN; Yang, LI; Shuaishuai, YAN; Jianguo, XU; and Xiaoli, PENG (2025) "3-Monochloropropane- 1,2-diol induced cell apoptosis in HK 2 cells via mitochondria-dependent pathway," Food and Machinery: Vol. 40: Iss. 11, Article 1.
DOI: 10.13652/j.spjx.1003.5788.2024.80442
Available at: https://www.ifoodmm.cn/journal/vol40/iss11/1

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