Optimization of the preparation process of hypotensive peptide of Agrocybe cylindracea

Authors

WU Jinyao, School of Public Health , Guizhou Medical University , Guiyang , Guizhou 550025 , China;The Key Laboratory of Environmental Pollution Monitoring and Disease Control , Ministry of Education , Guizhou Medical University , Guiyang , Guizhou 550025 , China
YIN Mingyue, School of Public Health , Guizhou Medical University , Guiyang , Guizhou 550025 , China;The Key Laboratory of Environmental Pollution Monitoring and Disease Control , Ministry of Education , Guizhou Medical University , Guiyang , Guizhou 550025 , China
YANG Meihua, School of Public Health , Guizhou Medical University , Guiyang , Guizhou 550025 , China;The Key Laboratory of Environmental Pollution Monitoring and Disease Control , Ministry of Education , Guizhou Medical University , Guiyang , Guizhou 550025 , China
KANG Jingjing, School of Public Health , Guizhou Medical University , Guiyang , Guizhou 550025 , China;The Key Laboratory of Environmental Pollution Monitoring and Disease Control , Ministry of Education , Guizhou Medical University , Guiyang , Guizhou 550025 , ChinaFollow

Corresponding Author(s)

康晶晶（1986—），女，贵州医科大学讲师，博士。E-mail：kangjingjing1027@126.com

Abstract

［Objective］ This study aimed to determine the optimal extraction process for antihypertensive peptide from Agrocybe cylindracea to achieve higher yield and enhanced activity.［Methods］ With the polypeptide yield and ACE inhibition rate as the evaluation index,the effects of enzyme type,substrate concentration,enzyme concentration,pH value,reaction time,reaction temperature and other single factors on the antihypertensive peptide and its activity were investigated,and the optimal extraction process of antihypertensive peptide was optimized by response surface.［Results］］ The optimal extraction conditions were as follows:substrate concentration of 5.4%,enzymolysis duration of 8 h,reaction temperature was 23.3 ℃,pH 8.0,and neutral protease dosage of 2 g.Under the control of these conditions,the antihypertensive peptide yield reached 92.12%,with an ACE inhibition rate of 93.75%.The active peptide fraction,primarily composed of peptides with molecular weight <3 kDa,accounted for 36.69% of the total peptide and demonstrated an ACE inhibition rate of 95.36%.［Conclusion］ The extraction process for antihypertensive peptides from A.cylindracea was successfully optimized,and the molecular weight distribution of the active peptide components was determined.

Publication Date

2-18-2025

First Page

172

Last Page

179

DOI

10.13652/j.spjx.1003.5788.2023.81169

Recommended Citation

Jinyao, WU; Mingyue, YIN; Meihua, YANG; and Jingjing, KANG (2025) "Optimization of the preparation process of hypotensive peptide of Agrocybe cylindracea," Food and Machinery: Vol. 40: Iss. 11, Article 24.
DOI: 10.13652/j.spjx.1003.5788.2023.81169
Available at: https://www.ifoodmm.cn/journal/vol40/iss11/24

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