Screening and identification of a bacteriocin-producing lactic acid bacterium and its antibacterial effect on Staphylococcus aureus

Authors

YANG Yangyang, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471023 , China
XU Yunfeng, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471023 , China
GUO Weiping, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471023 , China
LUO Lei, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471023 , China
XIANG Jinle, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471023 , ChinaFollow

Corresponding Author(s)

向进乐（1980—），男，河南科技大学教授，博士。E-mail：xjl5013@haust.edu.cn

Abstract

［Objective］ This study aimed to isolate and screen bacteriocin -producing lactic acid bacteria from kimchi,identify the strains and investigate their antibacterial effects against Staphylococcus aureus.［Methods］ S.aureus was employed as the indicator bacterium.Bacteriocin -producing strains were identified through acid elimination,hydrogen peroxide exclusion,and enzyme verification test.Physiological and biochemical identification,along with 16S rDNA sequencing,was conducted on the strains.The antibacterial properties of the bacteriocin extract were assessed using minimum inhibitory concentration (MIC ) determination,growth curve analysis,biofilm staining,and scanning electron microscopy observations.［Results］］ A bacteriocin -producing strain,designated N -11,was successfully isolated and identified as Lactobacillus plantarum.The MIC of the crude bacteriocin extract against S.aureus was found to be (17.50±0.25) mg/mL,demonstrating a significantly ability to inhibit both the growth and the biofilm formation of S.aureus.［Conclusion］ The crude extract producted by L.plantarum N-11 exhibits strong antibacterial effects against S.aureus,indicating its potential for further development and utilization in food preservation and safety applications.

Publication Date

2-18-2025

First Page

35

Last Page

40

DOI

10.13652/j.spjx.1003.5788.2024.80189

Recommended Citation

Yangyang, YANG; Yunfeng, XU; Weiping, GUO; Lei, LUO; and Jinle, XIANG (2025) "Screening and identification of a bacteriocin-producing lactic acid bacterium and its antibacterial effect on Staphylococcus aureus," Food and Machinery: Vol. 40: Iss. 11, Article 5.
DOI: 10.13652/j.spjx.1003.5788.2024.80189
Available at: https://www.ifoodmm.cn/journal/vol40/iss11/5

References

[1] KADARIYA J,SMITH T C,THAPALIYA D.Staphylococcus aureus and Staphylococcal food-borne disease:an ongoing challenge in public health [J].Biomed Research International,2014,2 014:827965.
[2] MOKOENA M.Lactic acid bacteria and their bacteriocins:classification,biosynthesis and applications against uropathogens:a mini-review [J].Molecules,2017,22(8):1 255.
[3] ZAPASNIK A,SOKOLOWSKA B,BRYLA M.Role of lactic acid bacteria in food preservation and safety [J].Foods,2022,11(9):1 283.
[4] CLEVELAND J,MONTVILLE T J,NES I F,et al.Bacteriocins:safe,natural antimicrobials for food preservation[J].International Journal of Food Microbiology,2001,71:1-20.
[5] PATO U,RIFTYAN E,AYU D F,et al.Antibacterial efficacy of lactic acid bacteria and bacteriocin isolated from Dadih's against Staphylococcus aureus [J].Food Science and Technology,2022,42:e27121.
[6] 孙海龙,李佳益,张敏,等.新疆特色果蔬中乳酸菌分离鉴定及其抑菌活性 [J].食品与生物技术学报,2018,37(11):1 201-1 206.SUN H L,LI Y J,ZHANG M,et al.Isolation and identification of lactic acid bacteria in characteristic fruits and vegetables of Xinjiang and their antibacterial activity [J].Journal of Food Science and Biotechnology,2018,37(11):1 201-1 206.
[7] BIKI S P,MAHMUD S,AKHTER S,et al.Bacteriocin production by Lactococcus lactis LL-HSTU-FPP strain isolated from fermented rice and evaluation of the biopreservation potentiality of bacteriocin on shrimp [J].Journal of Food Safety,2023,43(3):e13042.
[8] GARCIA A,BONILLA F,VILLASMIL E,et al.Antilisterial activity of freeze-dried bacteriocin-containing powders produced by lactic acid bacteria against Listeria innocua NRRL B-33016 on cantaloupe (Cucumis melo ) surface [J].LWT-Food Science and Technology,2022,154:112440.
[9] 李俊霞.产细菌素乳酸菌的筛选、鉴定及培养基的优化 [D].郑州:河南工业大学,2010:18.LI J X.The isolation,identification of lacticacid bacteria producing bacteriocin and the optimization of medium [D].Zhengzhou:Henan University of Technology,2010:18.
[10] 王娜,李慧,戴伶俐,等.产细菌素乳酸菌的筛选与鉴定 [J].中国食品学报,2020,20(12):248-255.WANG N,LI H,DAI L L,et al.Screening and identification of bacteriocin-producing lactic acid bacteria [J].Journal of Chinese Institute of Food Science and Technology,2020,20(12):248-255.
[11] 王朝,冉旋,雷江英,等.牦牛源产细菌素屎肠球菌的分离鉴定和益生特性 [J].微生物学通报,2023,50(8):3 454-3 466.WANG C,RAN X,LEI J Y,et al.Isolation,identification and probiotic properties of bacteriocin-producing Enterococcus faecium from yak [J].Microbiology China,2023,50(8):3 454-3 466.
[12] 王娜娜,梁宏彰,李婉,等.产细菌素乳酸菌的筛选及相关基因分析 [J].中国乳品工业,2016 (7):4-7.WANG N N,LIANG H Z,LI W,et al.Screening of bacteriocin-producing lactic acid bacteria and related genetic analysis [J].China Dairy Industry,2016 (7):4-7.
[13] DAI J Y,FANG L M,ZHANG M M,et al.Isolation and identification of new source of bacteriocin-producing Lactobacillus plantarum C010 and growth kinetics of its batch fermentation [J].World Journal of Microbiology and Biotechnology,2022,38(4):67.
[14] QIAN W D,LIU M,FU Y T,et al.Antimicrobial mechanism of luteolin against Staphylococcus aureus and Listeria monocytogenes and its antibiofilm properties [J].Microbial Pathogenesis,2020,142:104056.
[15] LIU M M,YANG K,WANG J,et al.Young astringent persimmon tannin inhibits methicillin-resistant Staphylococcus aureus isolated from pork [J].LWT-Food Science and Technology,2019,100:48-55.
[16] 徐云凤.安石榴苷对金黄色葡萄球菌的抑菌作用及机制研究[D].咸阳:西北农林科技大学,2017:49.XU Y F.Antimicrobial activities and mechanisms of punicalagin against Staphylococcus aureus [D].Xianyang:Northwest A & F University,2017:49.
[17] HE J F,LUO X G,JIN D X,et al.Identification,recombinant expression,and characterization of LGH 2,a novel antimicrobial peptide of Lactobacillus casei HZ 1[J].Molecules,2018,23(9):2 246.
[18] MANCUSKOVA T,MEDVEDOVA A,VALIK L.Production of antimicrobial substances by lactic acid bacteria [J].Chemicke Listy,2016,110(3):179-184.
[19] 乔柱.异源表达细菌素 BMP 32r杀菌和抑制生物膜作用机制研究 [D].咸阳:西北农林科技大学,2021:58-59.QIAO Z.The mechanism of heterologous expression of bacteriocin BMP 32r against bacteria and biofilm [D].Xianyang:Northwest A & F University,2021:58-59.
[20] VASILE C,BAICAN M.Progresses in food packaging,food quality,and safety-controlled-release antioxidant and/or antimicrobial packaging [J].Molecules,2021,26(5):1 263.
[21] ISLAM S,BISWAS S,JABIN T,et al.Probiotic potential of Lactobacillus plantarum DMR 14 for preserving and extending shelf life of fruits and fruit juice [J].Heliyon,2023,9(6):e17382.
[22] ZHANG L H,WANG L,YI L H,et al.A novel antimicrobial substance produced by Lactobacillus rhamnous LS8[J].Food Control,2017,73:754-760.