Abstract
［Objective］ This study aimed to establish a rapid and accurate analytical method for the determination of acrylamide in Xinjiang characteristic Naan products.［Methods］ The methodology involved adding the internal standard 13C3-acrylamide isotope to the samples,followed by the sequential addition of 9 mL of water,10 mL of acetonitrile,6 mL of n -hexane,6 g of anhydrous magnesium sulfate,and 1.5 g of sodium acetate salting agent.The samples were then extracted using vortex oscillation and centrifugation,discarding the n -hexane layer.Take 4 mL of the acetonitrile layer was purify with 400 mg of anhydrous magnesium sulfate and 300 mg PSA.The supernatant was evaporated to near dryness using liquid and subsequently re -dissolved in water for measurement.The separation was performed using ACQUITY UPLC HSS T 3 (100 mm ×2.1 mm,1.8 µm) column,with a mobile phase of water -methanol,in electrospray ionization positive ion mode and multi -reaction monitoring mode.［Results］］ The method demonstrated a strong linear relationship for acrylamide detection within the range of 1.0~100 ng/mL (R2>0.999 1),with detection and quantitation limits of 0.25 µg/kg and 0.82 µg/kg,respectively.Recovery tests at three different concentration levels in various sample matrices yielded an average recovery rate of 91.3%~97.6%,with RSD values ranging from 1.3%~4.6%.Analysis of 65 commercially available naan samples revealed acrylamide contents ranging from 17.4~207.5 µg/kg,with significant differences observed among different types of Naan (H=23.10,P<0.05).［Conclusion］ The proposed method features minimal sampling volume and reagent consumption,along with a rapid and straightforward process that yields accurate results.It is applicable to various types of naan products,demonstrating its versatility and efficiency.
Publication Date
2-18-2025
First Page
61
Last Page
68
DOI
10.13652/j.spjx.1003.5788.2024.80084
Recommended Citation
Man, XU; Shaolong, SONG; Jun, HU; Haiyun, SHI; and Jian, LIU
(2025)
"QuEChERS combined with ultra-high performance liquid chromatography-tandem mass spectrometry to determine acrylamide in Xinjiang Naan products,"
Food and Machinery: Vol. 40:
Iss.
11, Article 9.
DOI: 10.13652/j.spjx.1003.5788.2024.80084
Available at:
https://www.ifoodmm.cn/journal/vol40/iss11/9
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