Abstract
［Objective］ This study aimed to optimize the extraction process of polysaccharides from mulberry leaves using deep eutectic slovents (DES ) and assess their antioxidant properties.［Methods］ Autumn mulberry leaf powder was used as the raw material,and ultrasonic -assisted extraction with complex enzymes facilitated the process.The composition of DES and the extraction conditions were optimized using Box -Behnken response surface methodology,with polysaccharide yield as the primary indicator.Antioxidant activity was evaluated by measuring the scavenging abilities of against DPPH radicals,ABTS+ radicals,and hydroxyl radicals.［Results］］ The optimal extraction conditions were as follows:a choline chloride/malic acid molar ratio of 1∶4,water content 44%,liquid -solid ratio of 40∶1 （mL/g）,enzyme dosage of 3%,ultrasonic power of 350 W,and ultrasonic time 40 min.Under these conditions，the yield of mulberry leaf polysaccharides was （10.20±0.05）%.The polysaccharides demonstrated significan antioxidant activity,with a DPPH radical scavenging rate of 67.46% at 0.08 mg/mL and ABTS+ and hydroxyl radical scavenging rates of 87.19% and 93.44%,respectively,at 0.8 mg/mL.［Conclusion］ The ultrasonic -complex enzyme -assisted DES extraction method proved efficient,yielding high amounts of polysaccharides with strong antioxidant properties.These findings support the potential use of mulberry leaf polysaccharides in food and health -related applications.
Publication Date
2-18-2025
First Page
170
Last Page
177
DOI
10.13652/j.spjx.1003.5788.2024.80470
Recommended Citation
Jun, WU; Biwen, YANG; Pei, ZHAO; Jingqiu, MA; Xiaojing, WANG; and Yue, HUANG
(2025)
"Optimization of extraction process of polysaccharides from mulberry leaves and determination of the antioxidant activity in vitro,"
Food and Machinery: Vol. 40:
Iss.
12, Article 22.
DOI: 10.13652/j.spjx.1003.5788.2024.80470
Available at:
https://www.ifoodmm.cn/journal/vol40/iss12/22
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