Abstract
[Objective] This study aimed to investigate the composition,antioxidant properties,and stability of the essential oil extracts from Zanthoxylum armatum DC.[Methods] Essential oil was extracted using supercritical CO2 extraction (SFE-CO2) and steam distillation (HD).Gas chromatography -mass spectrometry (GC-MS) was employed to analyze the chemical composition of the oils.Antioxidant activity was evaluated using DPPH and ABTS free radical scavenging assays.Additionally,the effects of pH,temperature,preservatives,and sweeteners on the antioxidant activity of the essential oil were assessed.[Results]] GC-MS analysis revealed that alcohols and olefins were the primary components of the essential oils.Both extraction methods yielded oils with strong antioxidant properties.The half -maximal inhibition concentration (IC50) for ABTS radicals was 0.248 mg/mL for SFE -CO2 and 0.580 mg/mL for HD,while for DPPH radicals,the IC50 values were 4.620 mg/mL and 8.920 mg/mL.Variations in pH values,temperature,preservatives and sweeteners influenced the antioxidant activity of the oils.[Conclusion] The chemical composition of essential oils differs based on the extraction method,but both demonstrate strong antioxidant activity and stability under certain conditions.These findings suggest that Z.armatum essential oils could serve as a promising natural source of antioxidants.
Publication Date
2-18-2025
First Page
25
Last Page
31
DOI
10.13652/j.spjx.1003.5788.2024.80173
Recommended Citation
Mengjiao, ZHOU; Kai, CHEN; Lina, SUN; Feng, YI; and Xiaofeng, LIANG
(2025)
"Study on chemical components of the essential oil from Zanthoxylum armatum DC and its antioxidant activity,"
Food and Machinery: Vol. 40:
Iss.
12, Article 4.
DOI: 10.13652/j.spjx.1003.5788.2024.80173
Available at:
https://www.ifoodmm.cn/journal/vol40/iss12/4
References
[1] DEV A S,RAVINDRESH C,PUNEET J,et al.Nanoemulsions (O/W ) prepared from essential oil extracted from Melaleuca alternifolia:synthesis,characterization,stability and evaluation of anti-cancerous,anti-oxidant,anti-inflammatory and anti-diabetic activities [J].Journal of Biomaterials Science Polymer Edition,2023,34(17):2 438-2 461.
[2] GANOSI E,BARDA C,GRAFAKOU M E,et al.An in-depth stability study of the essential oils from Mentha×piperita,Mentha spicata,Origanum vulgare,and Thymus vulgaris:the impact of thermal and storage conditions [J].Separations,2023,10(9):488.
[3] 吴意,万娜,刘阳,等.中药挥发油稳定性影响因素、变化机制及保护策略 [J].中草药,2022,53(21):6 900-6 908.WU Y,WAN N,LIU Y,et al.Influencing factors,changing mechanisms and protection strategies of volatile oil from traditional Chinese medicine [J].Chinese Traditional and Herbal Drugs,2022,53(21):6 900-6 908.
[4] SUVARNA V,CHIPPA S.Current overview of cyclodextrin inclusion complexes of volatile oils and their constituents [J].Current Drug Delivery,2023,20(6):770-791.
[5] PENG L,ZHANG X F,GUO D Y,et al.Pickering emulsion technology based on the concept of "the combination of medicine and adjuvant" to enhance the oxidation stability of volatile oils in solid preparations-taking Lingzhu Pulvis as an example [J].RSC Advances,2022,12(42):27 453-27 462.
[6] WANG D Y,MENG Y D,ZHAO X M,et al.Sunflower oil flavored by essential oil from Punica granatum cv.Heyinshiliu peels improved its oxidative stability and sensory properties [J].LWT,2019,111:55-61.
[7] 周孟焦,何鑫柱,李朝俊,等.竹叶花椒黄酮抗氧化性及协同效应研究 [J].食品与机械,2023,39(5):139-143.ZHOU M J,HE X Z,LI C J,et al.Study on antioxidant activity and synergistic effect of flavonoids from Zanthoxylum armatum DC[J].Food & Machinery,2023,39(5):139-143.
[8] 刘先知.竹叶花椒花芽分化、果实形成及其发育特性研究 [D].成都:四川农业大学,2022:1-2.LIU X Z.Study on flower bud differentiation,fruit formation and developmental characteristics of Zanthoxylum armatum [D].Chengdu:Sichuan Agricultural University,2022:1-2.
[9] 申显当,蒙进芳,石卓功.山地竹叶花椒不同海拔不同类型枝条花芽分化差异 [J].中国瓜菜,2023,36(8):92-98.SHEN X D,MENG J F,SHI Z G.Differences in flower bud differentiation of different types of branches at different elevations of mountain Zanthoxylum armatum DC[J].China Cucurbits and Vegetables,2023,36(8):92-98.|[10] MA J,NING L P,WANG J Y,et al.GC-MS analysis and bioactivity screening of leaves and fruits of Zanthoxylum armatum DC[J].Separations,2023,10(8):420.
[11] 李潮俊.超临界 CO2萃取竹叶花椒精油及其抗氧化性研究[D].绵阳:西南科技大学,2023:19.LI C J.Study on supercritical CO2 extraction and antioxidation of essential oil of Zanthoxylum armatum DC[D].Mianyang:Southwest University of Science and Technology,2023:19.
[12] 何君强,熊雯宇,黄莹,等.灰树花醇提物化学成分及其生物活性研究 [J].食品与生物技术学报,2024,43(5):54-63.HE J Q,XIONG W Y,HUANG Y,et al.Chemical constituents and bioactivity of alcohol extracts from Grifola frondosa [J].Journal of Food Science and Biotechnology,2024,43(5):54-63.
[13] AYUB M A,CHOOBKAR N,HANIF M A,et al.Chemical composition,antioxidant,and antimicrobial activities of P.roxburghii oleoresin essential oils extracted by steam distillation,superheated steam,and supercritical fluid CO2 extraction [J].Journal of food science,2023,88(6):2 425-2 438.
[14] AL BAYATI M H M,CENGIZ M F,KITIS Y E,et al.Comparison of antimicrobial activities of oregano,lavender,sage,anise and clove extracts obtained by supercritical fluid carbon dioxide extraction and essential oils obtained by hydrodistillation [J].Journal of Essential Oil Research,2024,36(3):258-270.
[15] 肖新生,宋子龙,谢永燕,等.不同品种柚子皮精油成分及抗氧化与抑菌能力对比研究 [J].天然产物研究与开发,2024,36(2):218-226,267.XIAO X S,SONG Z L,XIE Y Y,et al.Comparison of components,antioxidant and bacteriostatic ability of different varieties of pomelo peel essential oil [J].Natural Product Research and Development,2024,36(2):218-226,267.
[16] LING Q Y,ZHANG B H,WANG Y B,et al.Chemical composition and antioxidant activity of the essential oils of citral-rich chemotype Cinnamomum camphora and Cinnamomum bodinieri [J].Molecules,2022,27(21):7 356.
[17] BASCHIERI A,AJVAZI M D,TONFACK J L F,et al.Explaining the antioxidant activity of some common non-phenolic components of essential oils [J].Food Chemistry,2017,232:656-663.
[18] ERDOGAN A,OZKAN A.Investigation of antioxıdative,cytotoxic,membrane-damaging and membrane-protective effects of the essential oil of Origanum majorana and its oxygenated monoterpene component linalool in human-derived Hep G 2 cell line [J].Iranian Journal of Pharmaceutical Research,2017,16(Suppl ):24-34.
[19] 于倩男.山梨酸的抑菌性、抗氧化性及其在烘焙产品中的应用[D].无锡:江南大学,2022:40-41.YU Q N.Antimicrobial and antioxidant properties of sorbic acid and its application in bakery products [D].Wuxi:Jiangnan University,2022:40-41.
[20] SHALABY E A,MAHMOUD G I,SHANAB S M M.Suggested mechanism for the effect of sweeteners on radical scavenging activity of phenolic compounds in black and green tea[J].Frontiers in Life Science,2016,9(4):241-251.